U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

hybO hydrogenase 2 small subunit [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945902, updated on 19-Nov-2025
Official Symbol
hybO
Official Full Name
hydrogenase 2 small subunit
Primary source
ECOCYC:G7554
Locus tag
b2997
See related
ASAP:ABE-0009834
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2991; yghV
Summary
Verified Tat substrate: [More information is available at EcoGene: EG13006]. HybO is the small subunit of hydrogenase 2, and it contains three Fe-S centers . [More information is available at EcoCyc: G7554].
Try the new Gene page
Try the new Transcripts and proteins table
Sequence:
NC_000913.3 (3145143..3146261, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene hydrogenase 2 maturation protease Neighboring gene hydrogenase 2 large subunit Neighboring gene hydrogenase 2 membrane subunit Neighboring gene hydrogenase 2 Neighboring gene DUF2623 domain-containing protein YghW Neighboring gene pseudo Neighboring gene protein YqhI

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Studies indicate that Hyd-1 and Hyd-2 are two H(2) uptake enzymes, while Hyd-1 is encoded by the first two genes of the hyaABCDEF operon, hyaA and hyaB, Hyd-2 is encoded by genes hybO and hybC of the hybOABCDEFG operon.
    Title: How Escherichia coli is equipped to oxidize hydrogen under different redox conditions.
  2. N-terminus verified by Edman degradation on mature peptide
    Title: Reassignment of the gene encoding the Escherichia coli hydrogenase 2 small subunit--identification of a soluble precursor of the small subunit in a hypB mutant.
  3. The CO and CN(-) ligands to the active site Fe in [NiFe]-hydrogenase of Escherichia coli have different metabolic origins.
    Title: The CO and CN(-) ligands to the active site Fe in [NiFe]-hydrogenase of Escherichia coli have different metabolic origins.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 3 iron, 4 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables 4 iron, 4 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables electron transfer activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ferredoxin hydrogenase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrogenase (acceptor) activity IEA
Inferred from Electronic Annotation
more info
  
enables iron-sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables iron-sulfur cluster binding ISM
Inferred from Sequence Model
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within anaerobic respiration EXP
Inferred from Experiment
more info
 PubMed 
involved_in anaerobic respiration IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
part_of [Ni-Fe] hydrogenase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of ferredoxin hydrogenase complex IEA
Inferred from Electronic Annotation
more info
  
located_in membrane HDA PubMed 
is_active_in membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in periplasmic space IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
Preferred Names
hydrogenase 2 small subunit
NP_417471.1

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3145143..3146261 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417471.1 hydrogenase 2 small subunit

    See identical proteins and their annotated locations for NP_417471.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A2X1MSQ3
    Conserved Domains (1) summary
    PRK10468
    Location:1 → 371
    PRK10468; hydrogenase 2 small subunit; Provisional
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.