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rssB [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945855, updated on 11-Sep-2024

Summary

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Official Symbol
rssB
Primary source
ECOCYC:EG12121
Locus tag
b1235
See related
ASAP:ABE-0004147
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1230; hnr; sprE; ychL
Summary
A yliE rssB9(hnr) double null mutant has significantly increased lycopene production in 1X M9; the effect may be salt-suppressible as it is not seen in 2X M9 (Alper, 2008). [More information is available at EcoGene: EG12121]. RssB facilitates and regulates degradation of sigma S by the protease ClpXP during normal nutrient conditions . [More information is available at EcoCyc: EG12121].
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Genomic context

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Sequence:
NC_000913.3 (1290242..1291255)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene NTF2-like domain-containing protein YchJ Neighboring gene putative patatin-like phospholipase RssA Neighboring gene UTP--glucose-1-phosphate uridylyltransferase Neighboring gene DNA-binding transcriptional dual regulator H-NS

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Phospho-dependent signaling during the general stress response by the atypical response regulator and ClpXP adaptor RssB. Schwartz J, et al. Protein Sci, 2021 Apr. PMID 33599047, Free PMC Article
  2. Insight into the RssB-Mediated Recognition and Delivery of σ(s) to the AAA+ Protease, ClpXP. Micevski D, et al. Biomolecules, 2020 Apr 16. PMID 32316259, Free PMC Article
  3. SprE levels are growth phase regulated in a sigma(S)-dependent manner at the level of translation. Gibson KE, et al. J Bacteriol, 2000 Jul. PMID 10869095, Free PMC Article
  4. Regulation of proteolysis of the stationary-phase sigma factor RpoS. Zhou Y, et al. J Bacteriol, 1998 Mar. PMID 9495753, Free PMC Article
  5. The response regulator SprE controls the stability of RpoS. Pratt LA, et al. Proc Natl Acad Sci U S A, 1996 Mar 19. PMID 8637901, Free PMC Article

See all (54) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Structure of phosphorylated-like RssB, the adaptor delivering sigma[s] to the ClpXP proteolytic machinery, reveals an interface switch for activation.
    Title: Structure of phosphorylated-like RssB, the adaptor delivering σ(s) to the ClpXP proteolytic machinery, reveals an interface switch for activation.
  2. Phospho-dependent signaling during the general stress response by the atypical response regulator and ClpXP adaptor RssB.
    Title: Phospho-dependent signaling during the general stress response by the atypical response regulator and ClpXP adaptor RssB.
  3. crystal structure of RssB bound to an antiadaptor, the DNA damage-inducible IraD; demonstrate that (1) in the presence of IraD, RssB adopts predominantly a closed conformation; (2) IraD contacts both domains of RssB; (3) the interactions observed in the structure are crucial for blocking sigmaS degradation in vivo and in vitro
    Title: Structural basis for inhibition of a response regulator of σ(S) stability by a ClpXP antiadaptor.
  4. Data propose that SprE plays a multifunctional role in controlling the transcriptome, regulating what is made via its effects on RpoS, and modulating what is degraded via its effects on polyadenylation and turnover of specific mRNAs.
    Title: The response regulator SprE (RssB) modulates polyadenylation and mRNA stability in Escherichia coli.
  5. IraD promotes accumulation of RpoS by binding to the adaptor RssB protein that targets RpoS for degradation.
    Title: A DNA damage response in Escherichia coli involving the alternative sigma factor, RpoS.
  6. IraM (inhibitor of RssB activity during Magnesium starvation) and IraD (inhibitor of RssB activity after DNA damage) inhibit sigmaS proteolysis both in vivo and in vitro.
    Title: Multiple pathways for regulation of sigmaS (RpoS) stability in Escherichia coli via the action of multiple anti-adaptors.
  7. The rssB mutant displays enhanced resistance to multiple external stresses, possibly indicating that the cell gains its increased vitality through elevated resistance to spontaneous, endogenous stresses.
    Title: Mutations in two global regulators lower individual mortality in Escherichia coli.
  8. Our data support the hypothesis that SprE activity is the key level at which ammonia and glucose starvation signals are transmitted to RpoS
    Title: Starvation for different nutrients in Escherichia coli results in differential modulation of RpoS levels and stability.
  9. phosphorylation site not required for regulation of RpoS proteolysis.
    Title: RpoS proteolysis is regulated by a mechanism that does not require the SprE (RssB) response regulator phosphorylation site.
  10. novel mechanism of regulation of sigma(S) stability through the regulation of RssB activity
    Title: Modulating RssB activity: IraP, a novel regulator of sigma(S) stability in Escherichia coli.
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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables phosphorelay response regulator activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables sigma factor antagonist activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables transcription cis-regulatory region binding IBA
Inferred from Biological aspect of Ancestor
more info
  
Process Evidence Code Pubs
involved_in negative regulation of DNA-templated transcription NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in phosphorelay signal transduction system IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within positive regulation of proteolysis EXP
Inferred from Experiment
more info
 PubMed 
acts_upstream_of_or_within positive regulation of proteolysis IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of proteolysis IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within protein destabilization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of DNA-templated transcription IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of gene expression IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of protein-DNA complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of sigma factor antagonist complex IPI
Inferred from Physical Interaction
more info
 PubMed 

General protein information

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Preferred Names
regulator of RpoS

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1290242..1291255
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415751.1 regulator of RpoS [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415751.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A2H4TM78
    Conserved Domains (2) summary
    PRK10693
    Location:36 → 337
    PRK10693; two-component system response regulator RssB
    cl19078
    Location:10 → 59
    REC; phosphoacceptor receiver (REC) domain of response regulators (RRs) and pseudo response regulators (PRRs)
Nucleotide Protein
Heading Accession and Version

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