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narI nitrate reductase A subunit gamma [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945808, updated on 6-Jul-2026
Official Symbol
narI
Official Full Name
nitrate reductase A subunit gamma
Primary source
ECOCYC:EG10640
Locus tag
b1227
See related
ASAP:ABE-0004125
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
chlI; ECK1221
Summary
NarI is involved in localizing NarGHI to the membrane Induced by anaerobiosis plus nitrate. [More information is available at EcoGene: EG10640]. The subunit of nitrate reductase A is a membrane-embedded heme-iron subunit resembling cytochrome b, which transfers electrons from the quinone pool to the subunit. [More information is available at EcoCyc: EG10640].
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Sequence:
NC_000913.3 (1285849..1286526)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene nitrate reductase A subunit alpha Neighboring gene nitrate reductase A subunit beta Neighboring gene nitrate reductase 1 molybdenum cofactor assembly chaperone Neighboring gene putative uncharacterized protein YchS Neighboring gene ncRNA Neighboring gene protamine-like protein

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. A new paradigm for electron transfer through Escherichia coli nitrate reductase A. Fedor JG, et al. Biochemistry, 2014 Jul 22. PMID 24960296
  2. Heme axial ligation by the highly conserved His residues in helix II of cytochrome b (NarI) of Escherichia coli nitrate reductase A. Magalon A, et al. J Biol Chem, 1997 Oct 10. PMID 9325288
  3. Sequence analysis of subunits of the membrane-bound nitrate reductase from a denitrifying bacterium: the integral membrane subunit provides a prototype for the dihaem electron-carrying arm of a redox loop. Berks BC, et al. Mol Microbiol, 1995 Jan. PMID 7746153
  4. Electron transfer from heme bL to the [3Fe-4S] cluster of Escherichia coli nitrate reductase A (NarGHI). Rothery RA, et al. Biochemistry, 2001 May 1. PMID 11318649
  5. Nitrate reductase and cytochrome bnitrate reductase structural genes as parts of the nitrate reductase operon. Bonnefoy-Orth V, et al. Mol Gen Genet, 1981. PMID 7022129

See all (51) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. A new paradigm for electron transfer through Escherichia coli nitrate reductase A.
    Title: A new paradigm for electron transfer through Escherichia coli nitrate reductase A.
  2. analysis of the role of the FS0 cluster of Escherichia coli nitrate reductase A (NarGHI) in enzyme maturation
    Title: Protein crystallography reveals a role for the FS0 cluster of Escherichia coli nitrate reductase A (NarGHI) in enzyme maturation.
  3. N-terminus verified by Edman degradation on complete protein
    Title: Roles of the narJ and narI gene products in the expression of nitrate reductase in Escherichia coli.
  4. molecular characterization of a quinol binding and oxidation site (Q-site) in NarGHI
    Title: Structural and biochemical characterization of a quinol binding site of Escherichia coli nitrate reductase A.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables electron transfer activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables heme binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables heme binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables nitrate reductase (quinone) activity IEA
Inferred from Electronic Annotation
more info
  
enables nitrate reductase activity EXP
Inferred from Experiment
more info
 PubMed 
enables nitrate reductase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables nitrate reductase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables nitrate reductase activity IEA
Inferred from Electronic Annotation
more info
  
enables nitrate reductase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in anaerobic electron transport chain IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in anaerobic electron transport chain IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in anaerobic electron transport chain IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within anaerobic respiration IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in nitrate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in nitrate metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
part_of NarGHI complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of NarGHI complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in membrane IDA
Inferred from Direct Assay
more info
 PubMed 
part_of nitrate reductase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of nitrate reductase complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of nitrate reductase complex IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane ISM
Inferred from Sequence Model
more info
 PubMed 
Preferred Names
nitrate reductase A subunit gamma
NP_415745.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1285849..1286526
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415745.1 nitrate reductase A subunit gamma

    See identical proteins and their annotated locations for NP_415745.1

    Status: REVIEWED

    Conserved Domains (1) summary
    TIGR00351
    Location:2 → 225
    narI; respiratory nitrate reductase, gamma subunit
Nucleotide Protein
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