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prmC protein-(glutamine-N(5)) methyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945779, updated on 17-Jul-2025
Official Symbol
prmC
Official Full Name
protein-(glutamine-N(5)) methyltransferase
Primary source
ECOCYC:EG12424
Locus tag
b1212
See related
ASAP:ABE-0004068
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1200; hemK
Summary
prmC(hemK) was originally isolated as a suppressor of the light sensitivity of hemH, now likely to be an indirect effect. [More information is available at EcoGene: EG12424]. PrmC is a protein-(glutamine-N5) methyltransferase that shows activity toward polypeptide chain release factors RF1 and RF2 . [More information is available at EcoCyc: EG12424].
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Sequence:
NC_000913.3 (1266094..1266927)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase Neighboring gene outer membrane lipoprotein LolB Neighboring gene glutamyl-tRNA reductase Neighboring gene peptide chain release factor RF1 Neighboring gene SirB family protein YchQ Neighboring gene transglutaminase-like/TPR repeat-containing protein Neighboring gene 3-deoxy-D-manno-octulosonate 8-phosphate synthase

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Structural characterization and comparative phylogenetic analysis of Escherichia coli HemK, a protein (N5)-glutamine methyltransferase. Yang Z, et al. J Mol Biol, 2004 Jul 16. PMID 15223314, Free PMC Article
  2. The methylator meets the terminator. Clarke S. Proc Natl Acad Sci U S A, 2002 Feb 5. PMID 11830650, Free PMC Article
  3. Molecular basis for bacterial class I release factor methylation by PrmC. Graille M, et al. Mol Cell, 2005 Dec 22. PMID 16364916
  4. Is the HemK family of putative S-adenosylmethionine-dependent methyltransferases a "missing" zeta subfamily of adenine methyltransferases? A hypothesis. Bujnicki JM, et al. IUBMB Life, 1999 Sep. PMID 10690633
  5. Methylation of bacterial release factors RF1 and RF2 is required for normal translation termination in vivo. Mora L, et al. J Biol Chem, 2007 Dec 7. PMID 17932046

See all (19) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. HemK is a Protein-glutamine N- methyltransferase
    Title: The hemK gene in Escherichia coli encodes the N(5)-glutamine methyltransferase that modifies peptide release factors.
  2. HemK is a Protein-glutamine N- methyltransferase
    Title: HemK, a class of protein methyl transferase with similarity to DNA methyl transferases, methylates polypeptide chain release factors, and hemK knockout induces defects in translational termination.
  3. Results describe the crystal structure of the complex between E. coli RF1 and PrmC bound to the methyl donor product AdoHCy.
    Title: Molecular basis for bacterial class I release factor methylation by PrmC.
Submit: New GeneRIF Correction

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables N-methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables S-adenosylmethionine-dependent methyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables S-adenosylmethionine-dependent methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein methyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein-glutamine N-methyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein-glutamine N-methyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in peptidyl-glutamine methylation IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within peptidyl-glutamine methylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein methylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of gene expression IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in translational termination IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in translational termination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Preferred Names
protein-(glutamine-N(5)) methyltransferase
NP_415730.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1266094..1266927
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415730.1 protein-(glutamine-N(5)) methyltransferase

    See identical proteins and their annotated locations for NP_415730.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0AAN5FWA8
    Conserved Domains (1) summary
    TIGR00536
    Location:1 → 277
    hemK_fam; HemK family putative methylases
Nucleotide Protein
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