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nfsB NAD(P)H-dependent nitroreductase NfsB [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945778, updated on 19-Nov-2025
Official Symbol
nfsB
Official Full Name
NAD(P)H-dependent nitroreductase NfsB
Primary source
ECOCYC:EG50005
Locus tag
b0578
See related
ASAP:ABE-0001981
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
dprA; ECK0570; nfnB; ntr
Summary
nfsA, nfsB, rutE, and ydjA are paralogs. [More information is available at EcoGene: EG20151]. Aspartate 37 has been implicated in binding the NADH cofactor. [More information is available at EcoCyc: EG50005].
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Sequence:
NC_000913.3 (604771..605424, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene phenylalanine:H(+) symporter PheP Neighboring gene miniconductance mechanosensitive channel YbdG Neighboring gene PF04237 family protein YbdF Neighboring gene DUF1158 domain-containing protein YbdJ

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. The bioactivation of 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB1954)--I. Purification and properties of a nitroreductase enzyme from Escherichia coli--a potential enzyme for antibody-directed enzyme prodrug therapy (ADEPT). Anlezark GM, et al. Biochem Pharmacol, 1992 Dec 15. PMID 1472094
  2. Generation of Escherichia coli nitroreductase mutants conferring improved cell sensitization to the prodrug CB1954. Grove JI, et al. Cancer Res, 2003 Sep 1. PMID 14500391
  3. The structure of Escherichia coli nitroreductase complexed with nicotinic acid: three crystal forms at 1.7 A, 1.8 A and 2.4 A resolution. Lovering AL, et al. J Mol Biol, 2001 May 25. PMID 11491290
  4. Sensitization of colorectal and pancreatic cancer cell lines to the prodrug 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB1954) by retroviral transduction and expression of the E. coli nitroreductase gene. Green NK, et al. Cancer Gene Ther, 1997 Jul-Aug. PMID 9253508
  5. Gene cloning, purification, and characterization of NfsB, a minor oxygen-insensitive nitroreductase from Escherichia coli, similar in biochemical properties to FRase I, the major flavin reductase in Vibrio fischeri. Zenno S, et al. J Biochem, 1996 Oct. PMID 8947835

See all (45) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Alterations in chromosomal genes nfsA, nfsB, and ribE are associated with nitrofurantoin resistance in Escherichia coli from the United Kingdom.
    Title: Alterations in chromosomal genes nfsA, nfsB, and ribE are associated with nitrofurantoin resistance in Escherichia coli from the United Kingdom.
  2. Mutations in nfsA and nfsB were the main mechanisms leading to nitrofurantoin resistance, and overexpression of the oqxAB gene might help to further increase the minimum inhibitory concentrations of nitrofurantoin.
    Title: Unravelling mechanisms of nitrofurantoin resistance and epidemiological characteristics among Escherichia coli clinical isolates.
  3. NfsA and NfsB, but not NemA, are responsible for the conversion of ethyl 2-(2'-nitrophenoxy)acetate.
    Title: Study of the role played by NfsA, NfsB nitroreductase and NemA flavin reductase from Escherichia coli in the conversion of ethyl 2-(2'-nitrophenoxy)acetate to 4-hydroxy-(2H)-1,4-benzoxazin-3(4H)-one (D-DIBOA), a benzohydroxamic acid with interesting biological properties.
  4. investigation of flavin-independent Fenton reaction system; NfnB and Fpr were purified and showed catalytic activities of ferric reduction; characterization of these enzymes and their involvement in the Fenton reaction and ferric reduction are presented
    Title: Escherichia coli ferredoxin-NADP+ reductase and oxygen-insensitive nitroreductase are capable of functioning as ferric reductase and of driving the Fenton reaction.
  5. N-terminus verified by Edman degradation on complete protein
    Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
  6. Data describe the interaction of Escherichia coli nitroreductase with the antibiotic nitrofurazone.
    Title: Structural and mechanistic studies of Escherichia coli nitroreductase with the antibiotic nitrofurazone. Reversed binding orientations in different redox states of the enzyme.
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Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 6,7-dihydropteridine reductase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 6,7-dihydropteridine reductase activity IEA
Inferred from Electronic Annotation
more info
  
enables FMN binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables NAD(P)H dehydrogenase (quinone) activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables oxidoreductase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in cytosol HDA PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane HDA PubMed 
Preferred Names
NAD(P)H-dependent nitroreductase NfsB
NP_415110.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    604771..605424 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415110.1 NAD(P)H-dependent nitroreductase NfsB

    See identical proteins and their annotated locations for NP_415110.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    F2X7N5
    Conserved Domains (1) summary
    PRK11053
    Location:1 → 217
    PRK11053; oxygen-insensitive NAD(P)H nitroreductase
Nucleotide Protein
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