nfsB [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: nfsB
Primary source: ASAP:ABE-0001981
Locus tag: b0578
See related: ECOCYC:EG50005
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: dprA; ECK0570; nfnB; ntr
Summary: nfsA, nfsB, rutE, and ydjA are paralogs. [More information is available at EcoGene: EG20151]. Aspartate 37 has been implicated in binding the NADH cofactor. [More information is available at EcoCyc: EG50005].
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Genomic context

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Sequence:: NC_000913.3 (604771..605424, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Alterations in chromosomal genes nfsA, nfsB, and ribE are associated with nitrofurantoin resistance in Escherichia coli from the United Kingdom.
Title: Alterations in chromosomal genes nfsA, nfsB, and ribE are associated with nitrofurantoin resistance in Escherichia coli from the United Kingdom.
Mutations in nfsA and nfsB were the main mechanisms leading to nitrofurantoin resistance, and overexpression of the oqxAB gene might help to further increase the minimum inhibitory concentrations of nitrofurantoin.
Title: Unravelling mechanisms of nitrofurantoin resistance and epidemiological characteristics among Escherichia coli clinical isolates.
NfsA and NfsB, but not NemA, are responsible for the conversion of ethyl 2-(2'-nitrophenoxy)acetate.
Title: Study of the role played by NfsA, NfsB nitroreductase and NemA flavin reductase from Escherichia coli in the conversion of ethyl 2-(2'-nitrophenoxy)acetate to 4-hydroxy-(2H)-1,4-benzoxazin-3(4H)-one (D-DIBOA), a benzohydroxamic acid with interesting biological properties.
investigation of flavin-independent Fenton reaction system; NfnB and Fpr were purified and showed catalytic activities of ferric reduction; characterization of these enzymes and their involvement in the Fenton reaction and ferric reduction are presented
Title: Escherichia coli ferredoxin-NADP+ reductase and oxygen-insensitive nitroreductase are capable of functioning as ferric reductase and of driving the Fenton reaction.
N-terminus verified by Edman degradation on complete protein
Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
Data describe the interaction of Escherichia coli nitroreductase with the antibiotic nitrofurazone.
Title: Structural and mechanistic studies of Escherichia coli nitroreductase with the antibiotic nitrofurazone. Reversed binding orientations in different redox states of the enzyme.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables 6,7-dihydropteridine reductase activity	IDA Inferred from Direct Assay more info	PubMed
enables 6,7-dihydropteridine reductase activity	IEA Inferred from Electronic Annotation more info
enables FMN binding	IDA Inferred from Direct Assay more info	PubMed
enables NAD(P)H dehydrogenase (quinone) activity	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor	IBA Inferred from Biological aspect of Ancestor more info
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in 2,4,6-trinitrotoluene catabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within 2,4,6-trinitrotoluene catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	HDA more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	HDA more info	PubMed

General protein information

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Preferred Names: NAD(P)H-dependent nitroreductase NfsB

NP_415110.1

EC 1.5.1.34

EC 1.6.5.2

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

604771..605424 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_415110.1 NAD(P)H-dependent nitroreductase NfsB [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_415110.1

Status: PROVISIONAL

UniProtKB/TrEMBL

F2X7N5

Conserved Domains (1) summary

PRK11053
Location:1 → 217

PRK11053; oxygen-insensitive NAD(P)H nitroreductase