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pth peptidyl-tRNA hydrolase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945765, updated on 26-Mar-2025
Official Symbol
pth
Official Full Name
peptidyl-tRNA hydrolase
Primary source
ECOCYC:EG10785
Locus tag
b1204
See related
ASAP:ABE-0004043
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1192; rap
Summary
Histidine 20 acts as the catalytic base during hydrolysis. [More information is available at EcoGene: EG10785]. Peptidyl-tRNA hydrolase is essential for growth in Escherichia coli. lysV, a lysine acceptor tRNA, was found to be a multicopy suppressor of a temperature sensitive pth mutant, indicating that the pth mutant phenotype is due to starvation for Lys-tRNA(Lys) . [More information is available at EcoCyc: EG10785].
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Sequence:
NC_000913.3 (1257929..1258513, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative autotransporter adhesin YcgV Neighboring gene redox-responsive ATPase YchF Neighboring gene stress-induced protein Neighboring gene aerobic C4-dicarboxylate transporter DauA

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Peptidyl-tRNA. VII. Substrate specificity of peptidyl-tRNA hydrolase. De Groot N, et al. Biochim Biophys Acta, 1969 Aug 20. PMID 4898482
  2. The fate of the initiator tRNAs is sensitive to the critical balance between interacting proteins. Thanedar S, et al. J Biol Chem, 2000 Jul 7. PMID 10748005
  3. Molecular basis for the temperature sensitivity of Escherichia coli pth(Ts). Cruz-Vera LR, et al. J Bacteriol, 2000 Mar. PMID 10692356, Free PMC Article
  4. Inhibition of translation and cell growth by minigene expression. Tenson T, et al. J Bacteriol, 1999 Mar. PMID 10049395, Free PMC Article
  5. Inhibition of Escherichia coli protein synthesis by abortive translation of phage lambda minigenes. Ontiveros C, et al. J Mol Biol, 1997 Jun 6. PMID 9191062

See all (47) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Study identifies the amino acid residues involved in the tRNA moiety recognition and also revealed the mechanism by which Pth accepts the diverse sequences of the elongator-tRNAs as substrate components.
    Title: Structural basis for the substrate recognition and catalysis of peptidyl-tRNA hydrolase.
  2. crystals of peptidyl-tRNA hydrolase belonged to the hexagonal space group P6(1), with unit-cell parameters a = b = 55.1, c = 413.1 A
    Title: Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Escherichia coli in complex with the acceptor-TΨC domain of tRNA.
  3. N-terminus verified by Edman degradation on complete protein
    Title: Peptidyl-tRNA hydrolase is involved in lambda inhibition of host protein synthesis.
  4. Possible mechanism for the suppression of thermosensitivity of pth(Ts) mutants through tRNALys overproduction.
    Title: Excess of charged tRNALys maintains low levels of peptidyl-tRNA hydrolase in pth(Ts) mutants at a non-permissive temperature.
Submit: New GeneRIF Correction

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables peptidyl-tRNA hydrolase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables peptidyl-tRNA hydrolase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables peptidyl-tRNA hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables tRNA binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within protein quality control for misfolded or incompletely synthesized proteins IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein quality control for misfolded or incompletely synthesized proteins IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within protein quality control for misfolded or incompletely synthesized proteins IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in rescue of stalled ribosome IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
peptidyl-tRNA hydrolase
NP_415722.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1257929..1258513 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415722.1 peptidyl-tRNA hydrolase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415722.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A232MCH0
    Conserved Domains (1) summary
    COG0193
    Location:2 → 192
    Pth; Peptidyl-tRNA hydrolase [Translation, ribosomal structure and biogenesis]
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

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