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icd isocitrate dehydrogenase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945702, updated on 30-Jul-2025
Official Symbol
icd
Official Full Name
isocitrate dehydrogenase
Primary source
ECOCYC:EG10489
Locus tag
b1136
See related
ASAP:ABE-0003823
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1122; icdA; icdE
Summary
This icd allele has a C-terminus added during prophage e14 integration at codon 362, introducing 2 amino acid changes in icd. icdC encodes the original C-terminus of icd. [More information is available at EcoGene: EG10489]. There are marked differences in the properties of enzymes from different sources. [More information is available at EcoCyc: EG10489].
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Sequence:
NC_000913.3 (1195123..1196373)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene phosphatase NudJ Neighboring gene 23S rRNA pseudouridine(2457) synthase Neighboring gene ncRNA Neighboring gene putative SAM-dependent methyltransferase YmfD

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. A highly active decarboxylating dehydrogenase with rationally inverted coenzyme specificity. Chen R, et al. Proc Natl Acad Sci U S A, 1995 Dec 5. PMID 8524825, Free PMC Article
  2. Metabolic characteristics of an isocitrate dehydrogenase defective derivative of escherichia coli BL21(DE3). Aoshima M, et al. Biotechnol Bioeng, 2003 Dec 20. PMID 14595786
  3. Thermosensitive phenotype of Escherichia coli mutant lacking NADP+-dependent isocitrate dehydrogenase. Choi IY, et al. Redox Rep, 2003. PMID 12631445
  4. Structural basis for a change in substrate specificity: crystal structure of S113E isocitrate dehydrogenase in a complex with isopropylmalate, Mg2+, and NADP. Doyle SA, et al. Biochemistry, 2001 Apr 10. PMID 11284679
  5. Sites of binding and orientation in a four-location model for protein stereospecificity. Mesecar AD, et al. IUBMB Life, 2000 May. PMID 10902579

See all (82) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. From a dimer to a monomer: Construction of a chimeric monomeric isocitrate dehydrogenase.
    Title: From a dimer to a monomer: Construction of a chimeric monomeric isocitrate dehydrogenase.
  2. GltA, icd and mdh do not form a transcription unit, are scattered around the chromosome and are expressed from different promoters.
    Title: Interaction of enzymes of the tricarboxylic acid cycle in Bacillus subtilis and Escherichia coli: a comparative study.
  3. introduced single point mutations (Arg153His, Arg153Cys); both the R153H and R153C mutants nearly lost wild-type IDH activity yet acquired the neomorphic ability to catalyze the conversion of alpha-ketoglutarate to 2-hydroxyglutarate
    Title: Point mutation (R153H or R153C) in Escherichia coli isocitrate dehydrogenase: Biochemical characterization and functional implication.
  4. Dimerization and bifunctionality confer robustness to the isocitrate dehydrogenase regulatory system in Escherichia coli
    Title: Dimerization and bifunctionality confer robustness to the isocitrate dehydrogenase regulatory system in Escherichia coli.
  5. N-terminus verified by Edman degradation on complete protein
    Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
  6. control of catalytic activity by phosphorylation [review]
    Title: Control of isocitrate dehydrogenase catalytic activity by protein phosphorylation in Escherichia coli.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables NAD binding IEA
Inferred from Electronic Annotation
more info
  
enables guanosine tetraphosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables isocitrate dehydrogenase (NADP+) activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables isocitrate dehydrogenase (NADP+) activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables isocitrate dehydrogenase (NADP+) activity IEA
Inferred from Electronic Annotation
more info
  
enables magnesium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within electron transport chain IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in glyoxylate cycle IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within response to oxidative stress IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in tricarboxylic acid cycle IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within tricarboxylic acid cycle IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in tricarboxylic acid cycle IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
isocitrate dehydrogenase
NP_415654.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1195123..1196373
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415654.1 isocitrate dehydrogenase

    See identical proteins and their annotated locations for NP_415654.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A5B9AN17, A0A5D8MS09, A0A8H9XRX7, A0A8S7JXI6
    Conserved Domains (1) summary
    PRK07006
    Location:8 → 416
    PRK07006; isocitrate dehydrogenase; Reviewed
Nucleotide Protein
Heading Accession and Version

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