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leuD 3-isopropylmalate dehydratase subunit LeuD [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945642, updated on 4-Feb-2026
Official Symbol
leuD
Official Full Name
3-isopropylmalate dehydratase subunit LeuD
Primary source
ECOCYC:EG11575
Locus tag
b0071
See related
ASAP:ABE-0000259
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0073
Summary
Isopropylmalate isomerase catalyzes the second step in leucine biosynthesis, converting 2-isopropylmalate to 3-isopropylmalate. [More information is available at EcoCyc: EG11575].
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Sequence:
NC_000913.3 (78848..79453, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene PtsG glucose transporter inhibitor Neighboring gene sugar exporter SetA Neighboring gene 3-isopropylmalate dehydratase subunit LeuC Neighboring gene 3-isopropylmalate dehydrogenase Neighboring gene 2-isopropylmalate synthase

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Heterologous expression and characterization of bacterial 2-C-methyl-D-erythritol-4-phosphate pathway in Saccharomyces cerevisiae. Carlsen S, et al. Appl Microbiol Biotechnol, 2013 Jul. PMID 23636690
  2. Salmonella typhimurium newD and Escherichia coli leuC genes code for a functional isopropylmalate isomerase in Salmonella typhimurium-Escherichia coli hybrids. Fultz PN, et al. J Bacteriol, 1979 Mar. PMID 374346, Free PMC Article
  3. Wild-type isopropylmalate isomerase in Salmonella typhimurium is composed of two different subunits. Fultz PN, et al. J Bacteriol, 1981 Oct. PMID 7026530, Free PMC Article
  4. Micromolar intracellular hydrogen peroxide disrupts metabolism by damaging iron-sulfur enzymes. Jang S, et al. J Biol Chem, 2007 Jan 12. PMID 17102132, Free PMC Article
  5. Genetic analysis of the leucine region in Escherichia coli B-r: gene-enzyme assignments. Yang HL, et al. J Bacteriol, 1974 Jan. PMID 4587614, Free PMC Article

See all (20) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. N-terminus verified by Edman degradation on mature peptide
    Title: Protein identification with N and C-terminal sequence tags in proteome projects.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 3-isopropylmalate dehydratase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 3-isopropylmalate dehydratase activity IEA
Inferred from Electronic Annotation
more info
  
enables lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in L-leucine biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in L-leucine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within L-leucine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in L-leucine biosynthetic process NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in amino acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in branched-chain amino acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of 3-isopropylmalate dehydratase complex EXP
Inferred from Experiment
more info
 PubMed 
part_of 3-isopropylmalate dehydratase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of 3-isopropylmalate dehydratase complex IEA
Inferred from Electronic Annotation
more info
  
part_of 3-isopropylmalate dehydratase complex IMP
Inferred from Mutant Phenotype
more info
 PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
3-isopropylmalate dehydratase subunit LeuD
NP_414613.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    78848..79453 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414613.1 3-isopropylmalate dehydratase subunit LeuD

    See identical proteins and their annotated locations for NP_414613.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A376FSH8
    Conserved Domains (1) summary
    PRK01641
    Location:3 → 200
    leuD; 3-isopropylmalate dehydratase small subunit
Nucleotide Protein
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