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hyaA hydrogenase 1 small subunit [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945579, updated on 19-Nov-2025
Official Symbol
hyaA
Official Full Name
hydrogenase 1 small subunit
Primary source
ECOCYC:EG10468
Locus tag
b0972
See related
ASAP:ABE-0003286
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0963
Summary
Verified Tat substrate: [More information is available at EcoGene: EG10468]. HyaA is the small subunit of hydrogenase 1. [More information is available at EcoCyc: EG10468].
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Sequence:
NC_000913.3 (1032139..1033257)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene modulator of FtsH protease Neighboring gene tRNA-Ser Neighboring gene hydrogenase 1 large subunit Neighboring gene hydrogenase 1 cytochrome b subunit Neighboring gene putative hydrogenase 1 maturation protease HyaD Neighboring gene putative HyaA chaperone Neighboring gene protein HyaF

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. X-ray structural, functional and computational studies of the O(2)-sensitive E. coli hydrogenase-1 C19G variant reveal an unusual [4Fe-4S] cluster. Volbeda A , et al. Chem Commun (Camb), 2018 Jun 26. PMID 29888350
  2. Assembly of membrane-bound respiratory complexes by the Tat protein-transport system. Sargent F, et al. Arch Microbiol, 2002 Aug. PMID 12115052
  3. Analysis of hydrogenase 1 levels reveals an intimate link between carbon and hydrogen metabolism in Escherichia coli K-12. Pinske C, et al. Microbiology (Reading), 2012 Mar. PMID 22241049
  4. A signal peptide that directs non-Sec transport in bacteria also directs efficient and exclusive transport on the thylakoid Delta pH pathway. Mori H, et al. J Biol Chem, 1998 May 8. PMID 9565548
  5. Importance of the Active Site "Canopy" Residues in an O(2)-Tolerant [NiFe]-Hydrogenase. Brooke EJ, et al. Biochemistry, 2017 Jan 10. PMID 28001048

See all (52) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The crystal structure of the Escherichia coli O2-sensitive C19G [NiFe]-hydrogenase-1 variant shows that the mutation results in a novel FeS cluster, proximal to the Ni-Fe active site.
    Title: X-ray structural, functional and computational studies of the O(2)-sensitive E. coli hydrogenase-1 C19G variant reveal an unusual [4Fe-4S] cluster.
  2. Studies indicate that Hyd-1 is encoded by the first two genes of the hyaABCDEF operon, hyaA and hyaB, and the arginine/lysine substitution lowers the H2 oxidation rate by nearly 2 orders of magnitude.
    Title: Importance of the Active Site "Canopy" Residues in an O(2)-Tolerant [NiFe]-Hydrogenase.
  3. Crystal structure of dimeric membrane-bound O(2)-tolerant hydrogenase 1 from Escherichia coli in a 2:1 complex with its physiological partner, cytochrome b.
    Title: Crystal structure of the O(2)-tolerant membrane-bound hydrogenase 1 from Escherichia coli in complex with its cognate cytochrome b.
  4. a combination of ArcA and AppY antirepression of IscR function, lower levels of IscR, and weak repression by [2Fe-2S]-IscR leads to increased hyaA promoter expression under anaerobic conditions
    Title: ArcA and AppY antagonize IscR repression of hydrogenase-1 expression under anaerobic conditions, revealing a novel mode of O2 regulation of gene expression in Escherichia coli.
  5. These results suggest that the ability to oxidize hydrogen by hydrogenase 1 shows a strong correlation with the rate of carbon flow through glycolysis and provides a direct link between hydrogen, carbon and energy metabolism.
    Title: Analysis of hydrogenase 1 levels reveals an intimate link between carbon and hydrogen metabolism in Escherichia coli K-12.
  6. Studies indicate that Hyd-1 and Hyd-2 are two H(2) uptake enzymes, while Hyd-1 is encoded by the first two genes of the hyaABCDEF operon, hyaA and hyaB, Hyd-2 is encoded by genes hybO and hybC of the hybOABCDEFG operon.
    Title: How Escherichia coli is equipped to oxidize hydrogen under different redox conditions.
  7. N-terminus verified by Edman degradation on mature peptide
    Title: Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 3 iron, 4 sulfur cluster binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables 3 iron, 4 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables 4 iron, 4 sulfur cluster binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables 4 iron, 4 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables electron transfer activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables electron transfer activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ferredoxin hydrogenase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrogenase (acceptor) activity IC
Inferred by Curator
more info
 PubMed 
enables hydrogenase (acceptor) activity IEA
Inferred from Electronic Annotation
more info
  
enables iron-sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within anaerobic electron transport chain IC
Inferred by Curator
more info
 PubMed 
involved_in anaerobic electron transport chain IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in anaerobic respiration IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in anaerobic respiration IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within anaerobic respiration IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in cellular response to starvation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in fermentation IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within fermentation IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within hydrogen metabolic process IC
Inferred by Curator
more info
 PubMed 
Component Evidence Code Pubs
part_of [Ni-Fe] hydrogenase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of [Ni-Fe] hydrogenase complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of [Ni-Fe] hydrogenase complex IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of [Ni-Fe] hydrogenase complex RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
part_of ferredoxin hydrogenase complex IEA
Inferred from Electronic Annotation
more info
  
is_active_in membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane ISM
Inferred from Sequence Model
more info
 PubMed 
located_in periplasmic side of plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
Preferred Names
hydrogenase 1 small subunit
NP_415491.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1032139..1033257
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415491.1 hydrogenase 1 small subunit

    See identical proteins and their annotated locations for NP_415491.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A6M9WX87
    Conserved Domains (1) summary
    TIGR00391
    Location:2 → 360
    hydA; hydrogenase (NiFe) small subunit (hydA)
Nucleotide Protein
Heading Accession and Version

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