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mukF [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945548, updated on 24-Aug-2024

Summary

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Official Symbol
mukF
Primary source
ECOCYC:EG12165
Locus tag
b0922
See related
ASAP:ABE-0003134
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0913; kicB
Summary
Condensin is required for chromosome partitioning and compaction. [More information is available at EcoGene: EG12165]. MukF plays a role in chromosome partitioning during cell division . [More information is available at EcoCyc: EG12165].
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Genomic context

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Sequence:
NC_000913.3 (974319..975641)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene PF03966 family protein YcaR Neighboring gene 3-deoxy-manno-octulosonate cytidylyltransferase Neighboring gene putative phosphotransferase YcbJ Neighboring gene envelope biogenesis factor Neighboring gene tRNA cmo(5)U34 methyltransferase Neighboring gene chromosome partitioning protein MukE Neighboring gene chromosome partitioning protein MukB Neighboring gene L,D-transpeptidase LdtD

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. MukB ATPases are regulated independently by the N- and C-terminal domains of MukF kleisin. Zawadzka K, et al. Elife, 2018 Jan 11. PMID 29323635, Free PMC Article
  2. MukE and MukF form two distinct high affinity complexes. Gloyd M, et al. J Biol Chem, 2007 May 11. PMID 17355972
  3. Intersubunit and intrasubunit interactions driving the MukBEF ATPase. Bahng S, et al. J Biol Chem, 2022 Jun. PMID 35452680, Free PMC Article
  4. Acyl carrier protein promotes MukBEF action in Escherichia coli chromosome organization-segregation. Prince JP, et al. Nat Commun, 2021 Nov 18. PMID 34795302, Free PMC Article
  5. Organization of the Escherichia coli Chromosome by a MukBEF Axial Core. Mäkelä J, et al. Mol Cell, 2020 Apr 16. PMID 32097603, Free PMC Article

See all (41) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Intersubunit and intrasubunit interactions driving the MukBEF ATPase.
    Title: Intersubunit and intrasubunit interactions driving the MukBEF ATPase.
  2. Acyl carrier protein promotes MukBEF action in Escherichia coli chromosome organization-segregation.
    Title: Acyl carrier protein promotes MukBEF action in Escherichia coli chromosome organization-segregation.
  3. Organization of the Escherichia coli Chromosome by a MukBEF Axial Core.
    Title: Organization of the Escherichia coli Chromosome by a MukBEF Axial Core.
  4. Complex formation requires functional interactions between the C- and N-terminal domains of MukF with the MukB head and neck, respectively, and MukE, which organizes the complexes by stabilizing binding of MukB heads to MukF. In the absence of head engagement, a MukF dimer bound by MukE forms complexes containing only a dimer of MukB.
    Title: Dynamic architecture of the Escherichia coli structural maintenance of chromosomes (SMC) complex, MukBEF.
  5. The authors show that a 4-helix bundle adjacent to the MukF dimerisation domain interacts functionally with the MukB coiled-coiled 'neck' adjacent to the ATPase head. They propose that this interaction leads to an asymmetric tripartite complex, as in other SMC (Structural Maintenance of Chromosomes) complexes.
    Title: MukB ATPases are regulated independently by the N- and C-terminal domains of MukF kleisin.
  6. activity of MukB requires DNA binding by the head domains of the protein but does not require either ATP or its partner proteins MukE or MukF. The ability of MukB to mediate DNA catenation underscores its potential for bringing distal regions of a chromosome together.
    Title: MukB-mediated Catenation of DNA Is ATP and MukEF Independent.
  7. MatP regulates the action of topoisomerase IV and MukBEF in chromosome segregation.
    Title: MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation.
  8. Taken together, the data implicate MukBEF as a key component of the DNA segregation process by acting in concert with TopoIV to promote decatenation and positioning of newly replicated oris.
    Title: The SMC complex MukBEF recruits topoisomerase IV to the origin of replication region in live Escherichia coli.
  9. MukBEF complexes may undergo multiple cycles of ATP hydrolysis without being released from DNA, analogous to the behavior of well-characterized molecular motors.
    Title: In vivo architecture and action of bacterial structural maintenance of chromosome proteins.
  10. findings show here that MukBEF generates and maintains the cellular positioning of chromosome loci independently of DNA replication.
    Title: The Escherichia coli SMC complex, MukBEF, shapes nucleoid organization independently of DNA replication.
Submit: New GeneRIF Correction See all GeneRIFs (13)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables calcium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA replication IEA
Inferred from Electronic Annotation
more info
  
involved_in cell division IEA
Inferred from Electronic Annotation
more info
  
involved_in chromosome condensation IEA
Inferred from Electronic Annotation
more info
  
involved_in chromosome condensation NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in chromosome segregation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of condensin complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in nucleoid IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
chromosome partitioning protein MukF

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    974319..975641
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415442.1 chromosome partitioning protein MukF [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415442.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A2X1IXE4, A0A2X1ND92, A0A9P2IB14
    Conserved Domains (1) summary
    PRK05260
    Location:1 → 440
    PRK05260; chromosome partition protein MukF
Nucleotide Protein
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