rpsA [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: rpsA
Primary source: ECOCYC:EG10900
Locus tag: b0911
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK0902; ssyF
Summary: HT_Cmplx22_Cyt: [More information is available at EcoGene: EG10900]. The S1 protein is essential in Escherichia coli; it is a component of the ribosome and is likely required for translation of most mRNAs . [More information is available at EcoCyc: EG10900].
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Genomic context

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Sequence:: NC_000913.3 (961995..963668)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structural insights into the binding of bS1 to the ribosome.
Title: Structural insights into the binding of bS1 to the ribosome.
An RNA pseudoknot is essential for standby-mediated translation of the tisB toxin mRNA in Escherichia coli.
Title: An RNA pseudoknot is essential for standby-mediated translation of the tisB toxin mRNA in Escherichia coli.
The ability of S1 to unfold the RNA is inversely correlated with the structural stability of the pseudoknot. These mechanistic insights delineate the scope and limitations of S1-chaperoned unfolding of structured RNAs.
Title: Protein unties the pseudoknot: S1-mediated unfolding of RNA higher order structure.
Single-particle cryo-electron microscopy structures of hibernating 70S and 100S particles isolated from stationary-phase E. coli reveal the presence of deacylated E-site transfer RNA and ribosomal protein bS1. A compact conformation of bS1 sequesters the anti-Shine-Dalgarno sequence of the 16S ribosomal RNA (rRNA), thereby inhibiting translation initiation.
Title: Structure of a hibernating 100S ribosome reveals an inactive conformation of the ribosomal protein S1.
r-protein S1 endows the 30S with an RNA chaperone activity that is essential for the docking and the unfolding of structured mRNAs, and for the correct positioning of the initiation codon inside the decoding channel
Title: Escherichia coli ribosomal protein S1 unfolds structured mRNAs onto the ribosome for active translation initiation.
Ribosomal protein S1 unwinds double-stranded RNA in multiple steps.
Title: Ribosomal protein S1 unwinds double-stranded RNA in multiple steps.
N-terminus verified by Edman degradation on complete protein
Title: Protein factors associated with the SsrA.SmpB tagging and ribosome rescue complex.
The last S1 motif is dispensable for translation and autoregulation: the defects seen with ssyF29 cells reflect an IS10R-mediated destabilization of the rpsA mRNA
Title: The deleterious effect of an insertion sequence removing the last twenty percent of the essential Escherichia coli rpsA gene is due to mRNA destabilization, not protein truncation.
S1 binding has a weak but detectable effect on the secondary structure of DsrA or rpoS mRNA.
Title: Interactions of ribosomal protein S1 with DsrA and rpoS mRNA.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables mRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables structural constituent of ribosome	IBA Inferred from Biological aspect of Ancestor more info
enables structural constituent of ribosome	IDA Inferred from Direct Assay more info	PubMed
enables structural constituent of ribosome	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within RNA secondary structure unwinding	IDA Inferred from Direct Assay more info	PubMed
involved_in cytoplasmic translation	NAS Non-traceable Author Statement more info	PubMed
acts_upstream_of_or_within negative regulation of cytoplasmic translation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of cytoplasmic translation	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within positive regulation of cytoplasmic translation	IEP Inferred from Expression Pattern more info	PubMed
involved_in ribosomal small subunit assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in translation	IBA Inferred from Biological aspect of Ancestor more info
involved_in translation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within translation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
part_of cytosolic small ribosomal subunit	IBA Inferred from Biological aspect of Ancestor more info
part_of cytosolic small ribosomal subunit	IDA Inferred from Direct Assay more info	PubMed
part_of cytosolic small ribosomal subunit	IPI Inferred from Physical Interaction more info	PubMed
located_in membrane	HDA more info	PubMed
part_of ribonucleoprotein complex	IEA Inferred from Electronic Annotation more info
located_in ribosome	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: 30S ribosomal subunit protein S1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

961995..963668

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_415431.1 30S ribosomal subunit protein S1 [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_415431.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A5C9AIT3

Conserved Domains (1) summary

PRK06299
Location:1 → 557

rpsA; 30S ribosomal protein S1; Reviewed