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pflA [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945517, updated on 24-Aug-2024

Summary

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Official Symbol
pflA
Primary source
ASAP:ABE-0003068
Locus tag
b0902
See related
ECOCYC:EG10028
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
act; ECK0893
Summary
Radical SAM superfamily. [More information is available at EcoGene: EG10028].
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Genomic context

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Sequence:
NC_000913.3 (950340..951080, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative DNA-binding transcriptional regulator YcaN Neighboring gene putative oxidoreductase YcaK Neighboring gene pyruvate formate-lyase Neighboring gene formate channel FocA

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Monovalent Cation Activation of the Radical SAM Enzyme Pyruvate Formate-Lyase Activating Enzyme. Shisler KA, et al. J Am Chem Soc, 2017 Aug 30. PMID 28768413, Free PMC Article
  2. Flavodoxin cofactor binding induces structural changes that are required for protein-protein interactions with NADP(+) oxidoreductase and pyruvate formate-lyase activating enzyme. Crain AV, et al. Biochim Biophys Acta, 2013 Dec. PMID 24016774, Free PMC Article
  3. The iron-sulfur cluster of pyruvate formate-lyase activating enzyme in whole cells: cluster interconversion and a valence-localized [4Fe-4S]2+ state. Yang J, et al. Biochemistry, 2009 Oct 6. PMID 19711960, Free PMC Article
  4. Reconstitution and characterization of the polynuclear iron-sulfur cluster in pyruvate formate-lyase-activating enzyme. Molecular properties of the holoenzyme form. Külzer R, et al. J Biol Chem, 1998 Feb 27. PMID 9478932
  5. Pyruvate formate-lyase (inactive form) and pyruvate formate-lyase activating enzyme of Escherichia coli: isolation and structural properties. Conradt H, et al. Arch Biochem Biophys, 1984 Jan. PMID 6364987

See all (25) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. N-terminus verified by Edman degradation on mature peptide
    Title: Primary structures of Escherichia coli pyruvate formate-lyase and pyruvate-formate-lyase-activating enzyme deduced from the DNA nucleotide sequences.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 4 iron, 4 sulfur cluster binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables 4 iron, 4 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables [formate-C-acetyltransferase]-activating enzyme activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables [formate-C-acetyltransferase]-activating enzyme activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
enables potassium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within DNA damage response IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in glucose metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within protein maturation IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
pyruvate formate-lyase activating enzyme
NP_415422.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    950340..951080 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415422.1 pyruvate formate-lyase activating enzyme [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415422.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A376FM59
    Conserved Domains (1) summary
    PRK11145
    Location:1 → 246
    pflA; pyruvate formate lyase 1-activating protein
Nucleotide Protein
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