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mlc DNA-binding transcriptional repressor Mlc [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945510, updated on 26-Nov-2024

Summary

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Official Symbol
mlc
Official Full Name
DNA-binding transcriptional repressor Mlc
Primary source
ECOCYC:G6852
Locus tag
b1594
See related
ASAP:ABE-0005323
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
dgsA; ECK1589
Summary
DgsA, better known as Mlc, "makes large colonies," is a transcriptional dual regulator that controls the expression of a number of genes encoding enzymes of the Escherichia coli phosphotransferase (PTS) and phosphoenolpyruvate (PEP) systems . [More information is available at EcoCyc: G6852].
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Genomic context

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Sequence:
NC_000913.3 (1667344..1668564, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene redox enzyme maturation protein DmsD Neighboring gene putative chloride:H(+) antiporter ClcB Neighboring gene dethiobiotin synthetase BidA Neighboring gene putative DNA-binding transcriptional regulator YnfL Neighboring gene putative transporter YnfM

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Dual inducer signal recognition by an Mlc homologue. Bréchemier-Baey D, et al. Microbiology (Reading), 2015 Aug. PMID 26293172
  2. Transcription regulators controlled by interaction with enzyme IIB components of the phosphoenolpyruvate: sugar phosphotransferase system. Joyet P, et al. Biochim Biophys Acta, 2013 Jul. PMID 23318733
  3. Decreasing accumulation of acetate in a rich medium by Escherichia coli on introduction of genes on a multicopy plasmid. Hosono K, et al. Biosci Biotechnol Biochem, 1995 Feb. PMID 7766024
  4. Purification of Mlc and analysis of its effects on the pts expression in Escherichia coli. Kim SY, et al. J Biol Chem, 1999 Sep 3. PMID 10464268
  5. Negative regulation of the pts operon by Mlc: mechanism underlying glucose induction in Escherichia coli. Tanaka Y, et al. Genes Cells, 1999 Jul. PMID 10469172

See all (37) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Mlc can be sequestered by both PtsG and NagE but that sequestration by NagE is only significant when the NagE level is high.
    Title: Dual inducer signal recognition by an Mlc homologue.
  2. This work identifies four amino acids in the linker region which allow Mlc to distinguish between a C/G and an A/T bp at positions +/-11 of the operators.
    Title: Operator recognition by the ROK transcription factor family members, NagC and Mlc.
  3. Studies indicate tht control mechanism of the repressor Mlc by interaction with enzyme IIB components of the phosphoenolpyruvate: sugar phosphotransferase system.
    Title: Transcription regulators controlled by interaction with enzyme IIB components of the phosphoenolpyruvate: sugar phosphotransferase system.
  4. Based on these findings, the authors propose a model of MtfA as a glucose-regulated peptidase activated by cytoplasmic Mlc.
    Title: Characterization of MtfA, a novel regulatory output signal protein of the glucose-phosphotransferase system in Escherichia coli K-12.
  5. Results show that regulation of ptsG by Mlc involves some cooperative binding of Mlc to the two Mlc operators upstream of ptsG.
    Title: Switching control of expression of ptsG from the Mlc regulon to the NagC regulon.
  6. The crystal structure of a tetrameric Mlc in a complex with four molecules of enzyme IIB(Glc) (EIIB), the cytoplasmic domain of EIICB(Glc), is reported.
    Title: Analyses of Mlc-IIBGlc interaction and a plausible molecular mechanism of Mlc inactivation by membrane sequestration.
  7. Repression by chimeric proteins Mlc confirms the importance of the C-terminal region of Mlc for both repression and inducer binding and show that the helix-turn-helix DNA-binding motif is not sufficient to interact with DNA.
    Title: Different regions of Mlc and NagC, homologous transcriptional repressors controlling expression of the glucose and N-acetylglucosamine phosphotransferase systems in Escherichia coli, are required for inducer signal recognition.
  8. crystal structure of Mlc, a global regulator of sugar metabolism
    Title: The crystal structure of Mlc, a global regulator of sugar metabolism in Escherichia coli.
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Pathways from PubChem

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General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA-templated transcription IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytoplasm TAS
Traceable Author Statement
more info
 PubMed 
located_in membrane NAS
Non-traceable Author Statement
more info
 PubMed 

General protein information

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Preferred Names
DNA-binding transcriptional repressor Mlc

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1667344..1668564 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416111.1 DNA-binding transcriptional repressor Mlc [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416111.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A377DS98
    Conserved Domains (2) summary
    COG1940
    Location:80 → 406
    NagC; Sugar kinase of the NBD/HSP70 family, may contain an N-terminal HTH domain [Transcription, Carbohydrate transport and metabolism]
    cl21459
    Location:20 → 60
    HTH; Helix-turn-helix domains
Nucleotide Protein
Heading Accession and Version

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