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ihfA [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945472, updated on 24-Aug-2024

Summary

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Official Symbol
ihfA
Primary source
ASAP:ABE-0005715
Locus tag
b1712
See related
ECOCYC:EG10440
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1710; hid; himA
Summary
The protein family is HI-HN-S The initiating methionine is cleaved . [More information is available at EcoCyc: EG10440].
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Genomic context

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Sequence:
NC_000913.3 (1795253..1795552, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene vitamin B12 ABC transporter ATP binding subunit Neighboring gene thioredoxin/glutathione peroxidase BtuE Neighboring gene vitamin B12 ABC transporter membrane subunit Neighboring gene phenylalanine--tRNA ligase subunit beta Neighboring gene phenylalanine--tRNA ligase subunit alpha

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The Role of Integration Host Factor in Escherichia coli Persister Formation. Nicolau SE, et al. mBio, 2022 Feb 22. PMID 34982597, Free PMC Article
  2. Integration host factor (IHF) modulates the expression of the pyrimidine-specific promoter of the carAB operons of Escherichia coli K12 and Salmonella typhimurium LT2. Charlier D, et al. Mol Gen Genet, 1993 Feb. PMID 8455562
  3. Activation of the Escherichia coli nitrate reductase (narGHJI) operon by NarL and Fnr requires integration host factor. Schröder I, et al. J Biol Chem, 1993 Jan 15. PMID 8419352
  4. Glucose starvation stimulon of Escherichia coli: role of integration host factor in starvation survival and growth phase-dependent protein synthesis. Nyström T. J Bacteriol, 1995 Oct. PMID 7559363, Free PMC Article
  5. Primary structure of the himA gene of Escherichia coli: homology with DNA-binding protein HU and association with the phenylalanyl-tRNA synthetase operon. Miller HI. Cold Spring Harb Symp Quant Biol, 1984. PMID 6397321

See all (139) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The Role of Integration Host Factor in Escherichia coli Persister Formation.
    Title: The Role of Integration Host Factor in Escherichia coli Persister Formation.
  2. H-NS, IHF, and DnaA lead to changes in nucleoid organizations, replication initiation, and cell division.
    Title: H-NS, IHF, and DnaA lead to changes in nucleoid organizations, replication initiation, and cell division.
  3. Cas1-Cas2-mediated spacer integration requires IHF-induced target DNA bending and explain the elusive role of CRISPR leader sequences during spacer acquisition and immunologic memory.
    Title: CRISPR Immunological Memory Requires a Host Factor for Specificity.
  4. Asymmetric positioning of Cas1-Cas2 complex and Integration Host Factor induced DNA bending guide the unidirectional homing of protospacer in CRISPR-Cas type I-E system.
    Title: Asymmetric positioning of Cas1-2 complex and Integration Host Factor induced DNA bending guide the unidirectional homing of protospacer in CRISPR-Cas type I-E system.
  5. Data show that environment dependent selection between integration host factors (IHF) and DNA-binding proteins from starved cells (Dps) results in different physical organizations of DNA.
    Title: Regulation of Bacterial DNA Packaging in Early Stationary Phase by Competitive DNA Binding of Dps and IHF.
  6. These results indicate that by combined transcriptional and translational controls of gene expression, Integration host factor activates expression of a specific set of genes required for survival at extremely acidic pH.
    Title: Integration host factor is required for the induction of acid resistance in Escherichia coli.
  7. Authors found that, although oligomers were assembled in the absence of any individual high-affinity DnaA binding site, loss of DnaA binding at peripheral sites eliminated Fis repression, and made binding of both Fis and IHF essential.
    Title: Building the bacterial orisome: high-affinity DnaA recognition plays a role in setting the conformation of oriC DNA.
  8. EcpR-mediated activation is aided by integration host factor (IHF), which is essential for counteracting the repression exerted by histone-like nucleoid-structuring protein (H-NS) on the ecp promoter.
    Title: Transcriptional regulation of the ecp operon by EcpR, IHF, and H-NS in attaching and effacing Escherichia coli.
  9. By performing ChIP-seq experiments of each subunit of IHF in the absence of the other subunit, genome-wide maps of DNA binding of the proteins in their hetero- and homodimeric forms were defined.
    Title: Genomic analysis of DNA binding and gene regulation by homologous nucleoid-associated proteins IHF and HU in Escherichia coli K12.
  10. Activity of TraI was enhanced by the relaxosome components TraM and integration host factor(ihfA and ihfB). The magnitude of stimulation depended on the proximity of the specific protein binding sites to the position of open DNA.
    Title: Protein and DNA effectors control the TraI conjugative helicase of plasmid R1.
Submit: New GeneRIF Correction See all GeneRIFs (14)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA-binding transcription activator activity IPI
Inferred from Physical Interaction
more info
 PubMed 
contributes_to sequence-specific DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables structural constituent of chromatin IEA
Inferred from Electronic Annotation
more info
  
enables transcription cis-regulatory region binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA recombination IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within DNA-templated transcription IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within DNA-templated transcription IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of translation IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of translation NAS
Non-traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
part_of IHF-DNA complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleoid IEA
Inferred from Electronic Annotation
more info
  
part_of protein-DNA complex IPI
Inferred from Physical Interaction
more info
 PubMed 

General protein information

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Preferred Names
integration host factor subunit alpha

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1795253..1795552 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416227.1 integration host factor subunit alpha [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416227.1

    Status: PROVISIONAL

    UniProtKB/Swiss-Prot
    P0A6X7
    Conserved Domains (1) summary
    PRK00285
    Location:1 → 97
    ihfA; integration host factor subunit alpha; Reviewed
Nucleotide Protein
Heading Accession and Version

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