exbB Ton complex subunit ExbB [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: exbB
Official Full Name: Ton complex subunit ExbB
Primary source: ECOCYC:EG10271
Locus tag: b3006
See related: ASAP:ABE-0009869
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK2999
Summary: ExbB and ExbD proteins function as part of the TonB-dependent energy transduction system for the import of iron-siderophore complexes and vitamin B12 across the outer membrane. [More information is available at EcoCyc: EG10271].
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Genomic context

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Sequence:: NC_000913.3 (3151250..3151984, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Single-particle cryo-electron microscopy shows that the ExbB pentamer encloses a dimer of ExbD in its transmembrane pore, and not a monomer as previously reported. Ton subcomplex consists of a pentamer of ExbB enclosing a dimer of ExbD TM domains. The essential Asp25 residues on ExbD are in close proximity to the conserved Thr 148 and 181 on ExbB.
Title: Cryo-EM structure of the bacterial Ton motor subcomplex ExbB-ExbD provides information on structure and stoichiometry.
Channel current measurement and 2D crystallography support the existence and transition of the two oligomeric states in membranes. The hexameric complex consists of six ExbB subunits and three ExbD transmembrane helices enclosed within the central channel.
Title: Hexameric and pentameric complexes of the ExbBD energizer in the Ton system.
ExbB-ExbD-TonB form complex in molar ratios of 4:1:1.
Title: Membrane Protein Complex ExbB4-ExbD1-TonB1 from Escherichia coli Demonstrates Conformational Plasticity.
A 10-residue-deletion scanning mutagenesis indicated that the cytoplasmic loop of ExbB (residues 40 to 129) was essential for TonB system activity.
Title: ExbB cytoplasmic loop deletions cause immediate, proton motive force-independent growth arrest.
Consideration of combined data excludes ExbB transmembrane domain residues from direct participation in a proton pathway.
Title: Mutations in Escherichia coli ExbB transmembrane domains identify scaffolding and signal transduction functions and exclude participation in a proton pathway.
ExbB(6) and ExbB(6)ExbD(1) are subcomplexes on which the final complex including TonB is assembled
Title: Oligomeric structure of ExbB and ExbB-ExbD isolated from Escherichia coli as revealed by LILBID mass spectrometry.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables energy transducer activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables identical protein binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transmembrane transporter activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cobalamin transport	NAS Non-traceable Author Statement more info	PubMed
acts_upstream_of_or_within ferrichrome import into cell	EXP Inferred from Experiment more info	PubMed
involved_in intracellular iron ion homeostasis	NAS Non-traceable Author Statement more info	PubMed
involved_in intracellular monoatomic cation homeostasis	NAS Non-traceable Author Statement more info	PubMed
involved_in protein import	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within protein stabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transmembrane transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cell outer membrane	NAS Non-traceable Author Statement more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	NAS Non-traceable Author Statement more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	RCA inferred from Reviewed Computational Analysis more info	PubMed
part_of plasma membrane protein complex	IDA Inferred from Direct Assay more info	PubMed