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exbB Ton complex subunit ExbB [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945420, updated on 19-Sep-2024

Summary

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Official Symbol
exbB
Official Full Name
Ton complex subunit ExbB
Primary source
ECOCYC:EG10271
Locus tag
b3006
See related
ASAP:ABE-0009869
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2999
Summary
ExbB and ExbD proteins function as part of the TonB-dependent energy transduction system for the import of iron-siderophore complexes and vitamin B12 across the outer membrane. [More information is available at EcoCyc: EG10271].
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Genomic context

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Sequence:
NC_000913.3 (3151250..3151984, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene NADP(+)-dependent aldehyde reductase Neighboring gene Ton complex subunit ExbD Neighboring gene cystathionine beta-lyase/L-cysteine desulfhydrase/alanine racemase Neighboring gene DedA family protein YghB

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. ExbB cytoplasmic loop deletions cause immediate, proton motive force-independent growth arrest. Bulathsinghala CM, et al. J Bacteriol, 2013 Oct. PMID 23913327, Free PMC Article
  2. Mutations in Escherichia coli ExbB transmembrane domains identify scaffolding and signal transduction functions and exclude participation in a proton pathway. Baker KR, et al. J Bacteriol, 2013 Jun. PMID 23603742, Free PMC Article
  3. Topology of the ExbB protein in the cytoplasmic membrane of Escherichia coli. Kampfenkel K, et al. J Biol Chem, 1993 Mar 15. PMID 8449962
  4. Excretion of enterochelin by exbA and exbB mutants of Escherichia coli. Guterman SK, et al. J Bacteriol, 1973 Jun. PMID 4576403, Free PMC Article
  5. Cryo-EM structure of the bacterial Ton motor subcomplex ExbB-ExbD provides information on structure and stoichiometry. Celia H, et al. Commun Biol, 2019. PMID 31602407, Free PMC Article

See all (61) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Single-particle cryo-electron microscopy shows that the ExbB pentamer encloses a dimer of ExbD in its transmembrane pore, and not a monomer as previously reported. Ton subcomplex consists of a pentamer of ExbB enclosing a dimer of ExbD TM domains. The essential Asp25 residues on ExbD are in close proximity to the conserved Thr 148 and 181 on ExbB.
    Title: Cryo-EM structure of the bacterial Ton motor subcomplex ExbB-ExbD provides information on structure and stoichiometry.
  2. Channel current measurement and 2D crystallography support the existence and transition of the two oligomeric states in membranes. The hexameric complex consists of six ExbB subunits and three ExbD transmembrane helices enclosed within the central channel.
    Title: Hexameric and pentameric complexes of the ExbBD energizer in the Ton system.
  3. ExbB-ExbD-TonB form complex in molar ratios of 4:1:1.
    Title: Membrane Protein Complex ExbB4-ExbD1-TonB1 from Escherichia coli Demonstrates Conformational Plasticity.
  4. A 10-residue-deletion scanning mutagenesis indicated that the cytoplasmic loop of ExbB (residues 40 to 129) was essential for TonB system activity.
    Title: ExbB cytoplasmic loop deletions cause immediate, proton motive force-independent growth arrest.
  5. Consideration of combined data excludes ExbB transmembrane domain residues from direct participation in a proton pathway.
    Title: Mutations in Escherichia coli ExbB transmembrane domains identify scaffolding and signal transduction functions and exclude participation in a proton pathway.
  6. ExbB(6) and ExbB(6)ExbD(1) are subcomplexes on which the final complex including TonB is assembled
    Title: Oligomeric structure of ExbB and ExbB-ExbD isolated from Escherichia coli as revealed by LILBID mass spectrometry.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables energy transducer activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transmembrane transporter activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in bacteriocin transport IEA
Inferred from Electronic Annotation
more info
  
involved_in cobalamin transport NAS
Non-traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within ferrichrome import into cell EXP
Inferred from Experiment
more info
 PubMed 
involved_in intracellular iron ion homeostasis NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in intracellular monoatomic cation homeostasis NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in protein import IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within protein stabilization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein transport IEA
Inferred from Electronic Annotation
more info
  
involved_in transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cell outer membrane NAS
Non-traceable Author Statement
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in membrane NAS
Non-traceable Author Statement
more info
 PubMed 
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
part_of plasma membrane protein complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of transmembrane transporter complex NAS
Non-traceable Author Statement
more info
 PubMed 

General protein information

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Preferred Names
Ton complex subunit ExbB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3151250..3151984 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417479.1 Ton complex subunit ExbB [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417479.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A827QSW3
    Conserved Domains (1) summary
    PRK10414
    Location:1 → 244
    PRK10414; biopolymer transporter ExbB
Nucleotide Protein
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