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galK galactokinase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945358, updated on 6-Jul-2026
Official Symbol
galK
Official Full Name
galactokinase
Primary source
ECOCYC:EG10363
Locus tag
b0757
See related
ASAP:ABE-0002568
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0746; galA
Summary
An amber mutation has been generated . [More information is available at EcoCyc: EG10363].
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Sequence:
NC_000913.3 (788831..789979, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 3-deoxy-7-phosphoheptulonate synthase, Phe-sensitive Neighboring gene 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase Neighboring gene galactose-1-epimerase Neighboring gene galactose-1-phosphate uridylyltransferase Neighboring gene UDP-glucose 4-epimerase

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Structure-based engineering of E. coli galactokinase as a first step toward in vivo glycorandomization. Yang J, et al. Chem Biol, 2005 Jun. PMID 15975511
  2. Studies on the substrate specificity of Escherichia coli galactokinase. Yang J, et al. Org Lett, 2003 Jun 26. PMID 12816414
  3. Enzyme formation in galactose-negative mutants of Escherichia coli. KURAHASHI K. Science, 1957 Jan 18. PMID 13390972
  4. Galactokinse from Escherichia coli. SHERMAN JR, et al. J Biol Chem, 1963 Mar. PMID 13977183
  5. Creation of the first anomeric D/L-sugar kinase by means of directed evolution. Hoffmeister D, et al. Proc Natl Acad Sci U S A, 2003 Nov 11. PMID 14612558, Free PMC Article

See all (41) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Single-mutations at the galactose-binding site of enzymes GalK, GalU, and LgtC enable the efficient synthesis of UDP-6-azido-6-deoxy-d-galactose and azido-functionalized Gb3 analogs.
    Title: Single-mutations at the galactose-binding site of enzymes GalK, GalU, and LgtC enable the efficient synthesis of UDP-6-azido-6-deoxy-d-galactose and azido-functionalized Gb3 analogs.
  2. type 2 polarity occurs as a result of the down-regulation of galK, which is caused by two mechanisms: Spot 42-mediated degradation of the galK-specific mRNA, mK2, and Spot 42-mediated Rho-dependent transcription termination at the end of galT
    Title: Two-level inhibition of galK expression by Spot 42: Degradation of mRNA mK2 and enhanced transcription termination before the galK gene.
  3. N-terminus verified by Edman degradation on mature peptide
    Title: The NH2-terminal sequences of galactokinase from Escherichia coli and Saccharomyces cerevisiae.
  4. 6-azido-6-deoxy-galactose and 6-azido-6-deoxy-glucose present unique chemical probes to assess the utility of an E. coli Y371H/M173L-GalK-overproducing strain to generate unnatural sugar-1-phosphates in vivo
    Title: Structure-based engineering of E. coli galactokinase as a first step toward in vivo glycorandomization.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables galactokinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables galactokinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables galactokinase activity IEA
Inferred from Electronic Annotation
more info
  
enables galactokinase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables magnesium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables phosphotransferase activity, alcohol group as acceptor IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within beta-D-galactose catabolic process via UDP-galactose, Leloir pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in carbohydrate phosphorylation IEA
Inferred from Electronic Annotation
more info
  
involved_in galactose metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in galactose metabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
galactokinase
NP_415278.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    788831..789979 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415278.1 galactokinase

    See identical proteins and their annotated locations for NP_415278.1

    Status: REVIEWED

    Conserved Domains (1) summary
    PRK05101
    Location:1 → 382
    PRK05101; galactokinase; Provisional
Nucleotide Protein
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