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nadA quinolinate synthase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945351, updated on 26-Mar-2025
Official Symbol
nadA
Official Full Name
quinolinate synthase
Primary source
ECOCYC:EG10630
Locus tag
b0750
See related
ASAP:ABE-0002541
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0739; nicA
Summary
C291 and C294 form a reversible disulfide bond that regulates NadA activity (Saunders, 2008). [More information is available at EcoGene: EG10630]. Quinolinate synthase catalyzes the second reaction in the de novo biosynthesis of NAD from aspartate, the condensation of iminosuccinate with dihydroxyacetone phosphate to form quinolinate. [More information is available at EcoCyc: EG10630].
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Sequence:
NC_000913.3 (782085..783128)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene tRNA-Lys Neighboring gene tRNA-Lys Neighboring gene nicotinamide riboside transporter Neighboring gene Zn(2(+))/Cd(2(+))/Ni(2(+))/Cu(2(+)) exporter Neighboring gene PF13985 family protein YbgS

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. The [4Fe-4S] cluster of quinolinate synthase from Escherichia coli: investigation of cluster ligands. Rousset C, et al. FEBS Lett, 2008 Aug 20. PMID 18674537
  2. Regulation of the activity of Escherichia coli quinolinate synthase by reversible disulfide-bond formation. Saunders AH, et al. Biochemistry, 2008 Aug 19. PMID 18651751, Free PMC Article
  3. Protein A of quinolinate synthetase is the site of oxygen poisoning of pyridine nucleotide coenzyme synthesis in Escherichia coli. Draczynska-Lusiak B, et al. Free Radic Biol Med, 1992 Dec. PMID 1459486
  4. Revised linkage map of Escherichia coli. Taylor AL, et al. Bacteriol Rev, 1967 Dec. PMID 4865540, Free PMC Article
  5. Escherichia coli quinolinate synthetase does indeed harbor a [4Fe-4S] cluster. Cicchillo RM, et al. J Am Chem Soc, 2005 May 25. PMID 15898769

See all (28) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. These studies reveal that only three cysteine residues, the conserved Cys113, Cys200 and Cys297, are ligands of the NadA [4Fe-4S] cluster.
    Title: The [4Fe-4S] cluster of quinolinate synthase from Escherichia coli: investigation of cluster ligands.
  2. NadA protein from E. coli was isolated and characterized.
    Title: Quinolinate synthetase, an iron-sulfur enzyme in NAD biosynthesis.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 4 iron, 4 sulfur cluster binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 4 iron, 4 sulfur cluster binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables 4 iron, 4 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables 4 iron, 4 sulfur cluster binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables iron-sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables quinolinate synthetase A activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables quinolinate synthetase A activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables quinolinate synthetase A activity IEA
Inferred from Electronic Annotation
more info
  
enables quinolinate synthetase A activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity, transferring alkyl or aryl (other than methyl) groups IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in 'de novo' NAD biosynthetic process from L-aspartate IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within 'de novo' NAD biosynthetic process from L-aspartate IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in NAD biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in pyridine nucleotide biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in quinolinate biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
quinolinate synthase
NP_415271.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    782085..783128
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415271.1 quinolinate synthase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415271.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A376FQI4, A4ZVM2
    Conserved Domains (1) summary
    PRK09375
    Location:9 → 343
    PRK09375; quinolinate synthase NadA
Nucleotide Protein
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