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nei endonuclease VIII [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945320, updated on 26-Mar-2025
Official Symbol
nei
Official Full Name
endonuclease VIII
Primary source
ECOCYC:G6383
Locus tag
b0714
See related
ASAP:ABE-0002433
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0703
Summary
Damaged bases in DNA occur due to alkylating agents, ionizing radiation or oxidants such as superoxide radicals, hydrogen peroxide and hydroxyl radicals. [More information is available at EcoCyc: G6383].
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Sequence:
NC_000913.3 (745935..746726)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 5-oxoprolinase component B Neighboring gene 5-oxoprolinase component C Neighboring gene 5-oxoprolinase component A Neighboring gene putative regulator Neighboring gene putative fimbrial protein YbgO Neighboring gene putative fimbrial chaperone YbgP Neighboring gene putative fimbrial usher protein YbgQ

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Conformational dynamics of the interaction of Escherichia coli endonuclease VIII with DNA substrates. Kuznetsov NA, et al. DNA Repair (Amst), 2012 Nov 1. PMID 23000248
  2. Isolation and characterization of endonuclease VIII from Escherichia coli. Melamede RJ, et al. Biochemistry, 1994 Feb 8. PMID 8110759
  3. Escherichia coli endonuclease VIII: cloning, sequencing, and overexpression of the nei structural gene and characterization of nei and nei nth mutants. Jiang D, et al. J Bacteriol, 1997 Jun. PMID 9171429, Free PMC Article
  4. Characterization of Escherichia coli endonuclease VIII. Jiang D, et al. J Biol Chem, 1997 Dec 19. PMID 9405426
  5. A novel role for Escherichia coli endonuclease VIII in prevention of spontaneous G-->T transversions. Blaisdell JO, et al. J Bacteriol, 1999 Oct. PMID 10515930, Free PMC Article

See all (32) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Requirements for DNA bubble structure for efficient cleavage by helix-two-turn-helix DNA glycosylases.
    Title: Requirements for DNA bubble structure for efficient cleavage by helix-two-turn-helix DNA glycosylases.
  2. A study of conformational changes in the Nei molecule when the enzyme binds normal DNA, damaged but uncleavable DNA, and several cleavable damaged DNA substrates.
    Title: Conformational dynamics of the interaction of Escherichia coli endonuclease VIII with DNA substrates.
  3. N-terminus verified by Edman degradation on mature peptide
    Title: Escherichia coli endonuclease VIII: cloning, sequencing, and overexpression of the nei structural gene and characterization of nei and nei nth mutants.
  4. Endonuclease VIII does not protect E.coli against possible mutations attributable to formamidopyrimidine lesions.
    Title: Excision of formamidopyrimidine lesions by endonucleases III and VIII is not a major DNA repair pathway in Escherichia coli.
  5. The structure of the uncomplexed Nei shows a significant conformational change compared with the Nei structure in its complex with DNA, reflecting a approximately 50 degrees rotation of the two main domains of the enzyme.
    Title: Structure of the uncomplexed DNA repair enzyme endonuclease VIII indicates significant interdomain flexibility.
  6. crystal structure
    Title: Crystallization and preliminary crystallographic analysis of endonuclease VIII in its uncomplexed form.
  7. Nei base excision repair enzyme is critical for the recognition and repair of the spiroiminodihydantoin lesion in bacterial cell lines
    Title: Nei deficient Escherichia coli are sensitive to chromate and accumulate the oxidized guanine lesion spiroiminodihydantoin.
Submit: New GeneRIF Correction

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables DNA N-glycosylase activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA-(apurinic or apyrimidinic site) endonuclease activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA-(apurinic or apyrimidinic site) endonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA-(apurinic or apyrimidinic site) endonuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables class I DNA-(apurinic or apyrimidinic site) endonuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables damaged DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables damaged DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, acting on glycosyl bonds IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, hydrolyzing N-glycosyl compounds IEA
Inferred from Electronic Annotation
more info
  
enables lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleic acid binding IEA
Inferred from Electronic Annotation
more info
  
enables oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in DNA damage response IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in base-excision repair IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within base-excision repair IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in base-excision repair IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
endonuclease VIII

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    745935..746726
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415242.1 endonuclease VIII [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415242.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A2X1NQB2
    Conserved Domains (1) summary
    PRK10445
    Location:1 → 263
    PRK10445; endonuclease VIII; Provisional
Nucleotide Protein
Heading Accession and Version

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