Format

Send to:

Choose Destination

seqA SeqA, negative modulator of initiation of replication [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945272, updated on 11-Oct-2018

Summary

Gene symbol
seqA
Gene description
SeqA, negative modulator of initiation of replication
Primary source
EcoGene:EG12197
Locus tag
b0687
See related
ASAP:ABE-0002343; ECOCYC:EG12197
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0675; hsm-1
Summary
SeqA is required for the membrane binding of hemimethylated oriC in vitro. [More information is available at EcoGene: EG12197]. The SeqA protein is a negative modulator of the initiation of chromosome replication and is required for sequestration of oriC, ensuring a single round of chromosome replication per cell cycle . [More information is available at EcoCyc: EG12197].

Genomic context

Sequence:
NC_000913.3 (712987..713532)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene LexA-regulated protein Neighboring gene esterase Neighboring gene phosphoglucomutase Neighboring gene lipoprotein YbfP

Bibliography

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

General gene information

Gene Ontology Provided by GO

Function Evidence Code Pubs
DNA replication origin binding IDA
Inferred from Direct Assay
more info
PubMed 
double-stranded methylated DNA binding IDA
Inferred from Direct Assay
more info
PubMed 
hemi-methylated DNA-binding IDA
Inferred from Direct Assay
more info
PubMed 
identical protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
Process Evidence Code Pubs
negative regulation of DNA-dependent DNA replication initiation IGI
Inferred from Genetic Interaction
more info
PubMed 
negative regulation of DNA-dependent DNA replication initiation IMP
Inferred from Mutant Phenotype
more info
PubMed 
nucleoid organization IGI
Inferred from Genetic Interaction
more info
PubMed 
protein homotetramerization IDA
Inferred from Direct Assay
more info
PubMed 
response to radiation IMP
Inferred from Mutant Phenotype
more info
PubMed 
sister chromatid cohesion IGI
Inferred from Genetic Interaction
more info
PubMed 
Component Evidence Code Pubs
SeqA-DNA complex IDA
Inferred from Direct Assay
more info
PubMed 
cytosol IDA
Inferred from Direct Assay
more info
PubMed 

General protein information

Preferred Names
SeqA, negative modulator of initiation of replication

NCBI Reference Sequences (RefSeq)

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    712987..713532
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415213.1 SeqA, negative modulator of initiation of replication [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415213.1

    Status: PROVISIONAL

    UniProtKB/Swiss-Prot
    P0AFY8
    Conserved Domains (1) summary
    PRK11187
    Location:1181
    PRK11187; replication initiation regulator SeqA; Provisional
Support Center