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lipB lipoyl(octanoyl) transferase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945217, updated on 19-Sep-2024

Summary

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Official Symbol
lipB
Official Full Name
lipoyl(octanoyl) transferase
Primary source
ECOCYC:EG11591
Locus tag
b0630
See related
ASAP:ABE-0002160
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
cde; ECK0623; lip
Summary
Revised TTG start is required for active protein. [More information is available at EcoGene: EG11591]. LipB is a lipoyl-protein ligase that specifically utilizes LipA-generated lipoyl-ACP, in contrast to the other lipoyl-protein ligase, LplA, which utilizes lipoate imported from outside the cell . [More information is available at EcoCyc: EG11591].
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Genomic context

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Sequence:
NC_000913.3 (661637..662278, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene lipoyl synthase Neighboring gene putative DNA-binding transcriptional regulator YbeF Neighboring gene DUF493 domain-containing protein YbeD Neighboring gene D-alanyl-D-alanine carboxypeptidase DacA

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Lipoate protein ligase B primarily recognizes the C(8)-phosphopantetheine arm of its donor substrate and weakly binds the acyl carrier protein. Dhembla C, et al. J Biol Chem, 2022 Aug. PMID 35764173, Free PMC Article
  2. The reaction of LipB, the octanoyl-[acyl carrier protein]:protein N-octanoyltransferase of lipoic acid synthesis, proceeds through an acyl-enzyme intermediate. Zhao X, et al. Biochemistry, 2005 Dec 20. PMID 16342964
  3. Cloning and characterization of the lipoyl-protein ligase gene LIPB from the yeast Kluyveromyces lactis: synergistic respiratory deficiency due to mutations in LIPB and mitochondrial F1-ATPase subunits. Chen XJ. Mol Gen Genet, 1997 Jul. PMID 9268025
  4. A new metabolic link. The acyl carrier protein of lipid synthesis donates lipoic acid to the pyruvate dehydrogenase complex in Escherichia coli and mitochondria. Jordan SW, et al. J Biol Chem, 1997 Jul 18. PMID 9218413
  5. Protein-protein interactions in assembly of lipoic acid on the 2-oxoacid dehydrogenases of aerobic metabolism. Hassan BH, et al. J Biol Chem, 2011 Mar 11. PMID 21209092, Free PMC Article

See all (30) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Lipoate protein ligase B primarily recognizes the C8-phosphopantetheine arm of its donor substrate and weakly binds the acyl carrier protein.
    Title: Lipoate protein ligase B primarily recognizes the C(8)-phosphopantetheine arm of its donor substrate and weakly binds the acyl carrier protein.
Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables acyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables lipoyl(octanoyl) transferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables lipoyl(octanoyl) transferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables lipoyl(octanoyl) transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within lipoate biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within lipoate metabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within negative regulation of gene expression IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein lipoylation IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within protein modification process IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
lipoyl(octanoyl) transferase
NP_415163.2

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    661637..662278 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415163.2 lipoyl(octanoyl) transferase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415163.2

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A2X1QDL9
    Conserved Domains (1) summary
    PRK14342
    Location:1 → 212
    PRK14342; lipoyl(octanoyl) transferase LipB
Nucleotide Protein
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