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purK 5-(carboxyamino)imidazole ribonucleotide synthase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945153, updated on 17-Jul-2025
Official Symbol
purK
Official Full Name
5-(carboxyamino)imidazole ribonucleotide synthase
Primary source
ECOCYC:EG10796
Locus tag
b0522
See related
ASAP:ABE-0001794
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0515; purE2
Summary
PurK was previously thought to be the ATPase subunit of phosphoribosylaminoimidazole carboxylase, with catalytic subunit PurE (Tiedeman, 1989). [More information is available at EcoGene: EG10796]. PurK and PurE were previously thought to be two subunits of AIR carboxylase , though more recent studies have shown the enzymes to be subunits of a distinct carboxylase and mutase, respectively . [More information is available at EcoCyc: EG10796].
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Sequence:
NC_000913.3 (551527..552594, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF1116 domain-containing protein YlbE Neighboring gene DUF2877 domain-containing protein YlbF Neighboring gene putative carbamate kinase Neighboring gene N(5)-carboxyaminoimidazole ribonucleotide mutase Neighboring gene UDP-2,3-diacylglucosamine diphosphatase Neighboring gene peptidyl-prolyl cis-trans isomerase B

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Structural analysis of the active site geometry of N5-carboxyaminoimidazole ribonucleotide synthetase from Escherichia coli. Thoden JB, et al. Biochemistry, 2008 Dec 16. PMID 19053251, Free PMC Article
  2. Structural and functional modularity of proteins in the de novo purine biosynthetic pathway. Li H, et al. Protein Sci, 2009 May. PMID 19384989, Free PMC Article
  3. Molecular cloning and characterization of the purE operon of Escherichia coli. Kamholz J, et al. Gene, 1986. PMID 3021590
  4. A DNA-binding protein with specificity for pur genes in Escherichia coli. Koduri RK, et al. J Biol Chem, 1980 Oct 25. PMID 7000760
  5. Reactions catalyzed by 5-aminoimidazole ribonucleotide carboxylases from Escherichia coli and Gallus gallus: a case for divergent catalytic mechanisms. Firestine SM, et al. Biochemistry, 1994 Oct 4. PMID 7918411

See all (19) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Report is given of X-ray structures of N5-carboxyaminoimidazole ribonucleotide synthetase (CAIR) with either magnesium (Mg)ATP or MgADP/inorganic phosphorus (Pi) bound in the enzyme's active site cleft.
    Title: Structural analysis of the active site geometry of N5-carboxyaminoimidazole ribonucleotide synthetase from Escherichia coli.
  2. N-terminus verified by Edman degradation on complete protein
    Title: Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 5-(carboxyamino)imidazole ribonucleotide synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ATP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables phosphoribosylaminoimidazole carboxylase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables phosphoribosylaminoimidazole carboxylase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity IPI
Inferred from Physical Interaction
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
5-(carboxyamino)imidazole ribonucleotide synthase
NP_415055.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    551527..552594 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415055.1 5-(carboxyamino)imidazole ribonucleotide synthase

    See identical proteins and their annotated locations for NP_415055.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A2X1PIY8, A0A3L5HCD8
    Conserved Domains (1) summary
    COG0026
    Location:1 → 350
    PurK; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase) [Nucleotide transport and metabolism]
Nucleotide Protein
Heading Accession and Version

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