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ispA geranyl diphosphate/farnesyl diphosphate synthase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945064, updated on 17-Jul-2025
Official Symbol
ispA
Official Full Name
geranyl diphosphate/farnesyl diphosphate synthase
Primary source
ECOCYC:EG10508
Locus tag
b0421
See related
ASAP:ABE-0001463
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0415
Summary
Farnesyl diphosphate synthase can utilize both dimethylallyl and geranyl diphosphates as substrates, generating geranyl and farnesyl diphosphate, respectively. [More information is available at EcoCyc: EG10508].
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Sequence:
NC_000913.3 (440202..441101, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 1-deoxyxylulose-5-phosphate synthase YajO Neighboring gene 1-deoxy-D-xylulose-5-phosphate synthase Neighboring gene exodeoxyribonuclease VII subunit XseB Neighboring gene tRNA uridine 4-sulfurtransferase

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Geranyl diphosphate synthase: an important regulation point in balancing a recombinant monoterpene pathway in Escherichia coli. Zhou J, et al. Enzyme Microb Technol, 2015 Jan. PMID 25435505
  2. A mutation of ispA that is involved in isoprenoid biogenesis can improve growth of Escherichia coli at low temperatures. Shiomi D, et al. Microbiol Immunol, 2011 Dec. PMID 22003871
  3. Short-chain prenyl diphosphate synthase that condenses isopentenyl diphosphate with dimethylallyl diphosphate in ispA null Escherichia coli strain lacking farnesyl diphosphate synthase. Saito K, et al. J Biosci Bioeng, 2007 Jun. PMID 17630132
  4. Isoprenoid biosynthesis as a drug target: bisphosphonate inhibition of Escherichia coli K12 growth and synergistic effects of fosmidomycin. Leon A, et al. J Med Chem, 2006 Dec 14. PMID 17149863
  5. Farnesyl diphosphate synthase: the art of compromise between substrate selectivity and stereoselectivity. Thulasiram HV, et al. J Am Chem Soc, 2006 Dec 13. PMID 17147392, Free PMC Article

See all (29) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The expression of GPPS was optimized by using ribosomal binding sites designed to have different translation initiation rates. GPPS is an important regulation point in balancing a recombinant geraniol synthesis pathway.
    Title: Geranyl diphosphate synthase: an important regulation point in balancing a recombinant monoterpene pathway in Escherichia coli.
  2. cold-sensitive growth in the rodZ mutant was suppressed by a mutation of ispA and the mutation improved cell growth of the wild-type at low temperatures
    Title: A mutation of ispA that is involved in isoprenoid biogenesis can improve growth of Escherichia coli at low temperatures.
  3. N-terminus verified by Edman degradation on complete protein
    Title: '98 Escherichia coli SWISS-2DPAGE database update.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables (2E,6E)-farnesyl diphosphate synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables dimethylallyltranstransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables prenyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables prenyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within farnesyl diphosphate biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within geranyl diphosphate biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
geranyl diphosphate/farnesyl diphosphate synthase
NP_414955.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    440202..441101 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414955.1 geranyl diphosphate/farnesyl diphosphate synthase

    See identical proteins and their annotated locations for NP_414955.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A8S7IJV8
    Conserved Domains (1) summary
    PRK10581
    Location:1 → 299
    PRK10581; (2E,6E)-farnesyl diphosphate synthase
Nucleotide Protein
Heading Accession and Version

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