phoB DNA-binding transcriptional dual regulator PhoB [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: phoB
Official Full Name: DNA-binding transcriptional dual regulator PhoB
Primary source: ECOCYC:EG10728
Locus tag: b0399
See related: ASAP:ABE-0001389
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK0393; phoRc; phoT
Summary: The PhoB protein belongs to the two-component family. [More information is available at EcoCyc: EG10728].
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Genomic context

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Sequence:: NC_000913.3 (417142..417831)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The results point to a model showing that PhoB is a component in the signal cascade regulating biofilm formation triggered by fluctuations of Polyphosphate levels in E. coli cells during stationary phase.
Title: PhoB activation in non-limiting phosphate condition by the maintenance of high polyphosphate levels in the stationary phase inhibits biofilm formation in Escherichia coli.
The expression timing of the core set of PhoB-regulated genes correlates well with the binding affinity of phosphorylated PhoB~P to individual promoters.
Title: Temporal hierarchy of gene expression mediated by transcription factor binding affinity and activation dynamics.
a combination of single-molecule and ensemble methods was used to investigate the binding mechanism and the impact of dimerization on the protein-DNA complex stability of PhoB(DBD)
Title: Cooperative binding of PhoB(DBD) to its cognate DNA sequence-a combined application of single-molecule and ensemble methods.
Data indicate that dimerization constants for the PhoB heterodimer and homodimer (two phosphorylated monomers) were similar, but the rate constant for heterodimer phosphorylation was ~10-fold higher than for the monomer.
Title: A link between dimerization and autophosphorylation of the response regulator PhoB.
sigma(4) domain of RpoD achieves promoter recognition through the N-terminal portion of its DNA recognition helix. Unexpectedly, the same recognition helix contacts the transactivation loop and helices alpha2 and alpha3 of PhoB.
Title: The structure of a transcription activation subcomplex reveals how σ(70) is recruited to PhoB promoters.
phosphorylation promotes dimerization via the alpha 4-beta 5-alpha 5 face, which enhances DNA binding and thus the ability of PhoB to regulate transcription.
Title: Probing the roles of the two different dimers mediated by the receiver domain of the response regulator PhoB.
CreC showed an interaction with PhoB, before an interaction between PhoB and PhoR, suggesting that this acts as a constant sensor for the early response to phosphate starvation.
Title: Transcript and protein level analyses of the interactions among PhoB, PhoR, PhoU and CreC in response to phosphate starvation in Escherichia coli.
Crystallography and solution NMR data for the receiver domain of PhoB which show distinct 2-fold symmetric dimers in the inactive and active states.
Title: Mechanism of activation for transcription factor PhoB suggested by different modes of dimerization in the inactive and active states.
PhoB has a role as a global regulator in Escherichia coli gene expression.
Title: Novel gene members in the Pho regulon of Escherichia coli.
analysis of cofactor-induced pre-active conformation in PhoB
Title: The cofactor-induced pre-active conformation in PhoB.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables bacterial-type RNA polymerase holo enzyme binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables phosphorelay response regulator activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphorelay response regulator activity	IEA Inferred from Electronic Annotation more info
enables transcription cis-regulatory region binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in phosphate ion transport	IEA Inferred from Electronic Annotation more info
involved_in phosphorelay signal transduction system	IEA Inferred from Electronic Annotation more info
involved_in regulation of DNA-templated transcription	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of DNA-templated transcription initiation	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
part_of protein-DNA complex	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: DNA-binding transcriptional dual regulator PhoB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

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Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

417142..417831

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_414933.1 DNA-binding transcriptional dual regulator PhoB [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_414933.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A376K1T1

Conserved Domains (1) summary

PRK10161
Location:1 → 229

PRK10161; phosphate response regulator transcription factor PhoB