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rsmA 16S rRNA m(6)2A1518,m(6)2A1519 dimethyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944939, updated on 26-Mar-2025

Summary

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Official Symbol
rsmA
Official Full Name
16S rRNA m(6)2A1518,m(6)2A1519 dimethyltransferase
Primary source
ECOCYC:EG10523
Locus tag
b0051
See related
ASAP:ABE-0000175
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0052; ksgA
Summary
RsmA(KsgA) dimethylates two adjacent 16S rRNA adenosines. rsmA(ksgA) deletion mutants grow more slowly than an isogenic wt at 20C (Connolly, 2008). [More information is available at EcoGene: EG10523]. KsgA is the methyltransferase responsible for dimethylation of 16S rRNA at the two adjacent adenosine bases A1518 and A1519 . [More information is available at EcoCyc: EG10523].
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Genomic context

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Sequence:
NC_000913.3 (51609..52430, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene diadenosine tetraphosphatase Neighboring gene DUF525 domain-containing protein ApaG Neighboring gene 4-hydroxythreonine-4-phosphate dehydrogenase Neighboring gene chaperone SurA Neighboring gene lipopolysaccharide assembly protein LptD

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Structural basis of successive adenosine modifications by the conserved ribosomal methyltransferase KsgA. Stephan NC, et al. Nucleic Acids Res, 2021 Jun 21. PMID 34086932, Free PMC Article
  2. Crystallization and preliminary X-ray diffraction analysis of KsgA, a universally conserved RNA adenine dimethyltransferase in Escherichia coli. O'Farrell HC, et al. Acta Crystallogr D Biol Crystallogr, 2003 Aug. PMID 12876362
  3. Crystal structure of KsgA, a universally conserved rRNA adenine dimethyltransferase in Escherichia coli. O'Farrell HC, et al. J Mol Biol, 2004 May 28. PMID 15136037
  4. Some properties of the ribosomal RNA methyltransferase encoded by ksgA and the polarity of ksgA transcription. Andrésson OS, et al. Mol Gen Genet, 1980. PMID 7005624
  5. The adenosine dimethyltransferase KsgA recognizes a specific conformational state of the 30S ribosomal subunit. Desai PM, et al. Arch Biochem Biophys, 2006 May 15. PMID 16620761

See all (43) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Structural basis of successive adenosine modifications by the conserved ribosomal methyltransferase KsgA.
    Title: Structural basis of successive adenosine modifications by the conserved ribosomal methyltransferase KsgA.
  2. Overexpression of RbfA in the absence of the KsgA results in impaired translation initiation.
    Title: Overexpression of RbfA in the absence of the KsgA checkpoint results in impaired translation initiation.
  3. A key aspect of KsgA's mechanism for regulating ribosome biogenesis is the ability of the enzyme to dimethylate its two target adenosines.
    Title: Control of substrate specificity by a single active site residue of the KsgA methyltransferase.
  4. Helix 24 and helix 45 of KsgA play important roles in catalysis of 16S rRNA.
    Title: Site-directed mutants of 16S rRNA reveal important RNA domains for KsgA function and 30S subunit assembly.
  5. KsgA functions as a late-stage ribosome biogenesis factor, and methylation is a trigger for release of KsgA from the assembling subunits. Gene deletion leads to cold sensitivity and altered ribosomal profiles.
    Title: Mechanistic insight into the ribosome biogenesis functions of the ancient protein KsgA.
  6. This study suggests that KsgA has another unidentified function that plays a role in the suppression of the cold-sensitive phenotype of the Era(E200K) strain and that this function may be involved in the acid shock response.
    Title: Dissection of 16S rRNA methyltransferase (KsgA) function in Escherichia coli.
Submit: New GeneRIF Correction

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables double-stranded DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables mRNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables rRNA (adenine) methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables rRNA (adenine-N6,N6-)-dimethyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables rRNA (adenine-N6,N6-)-dimethyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables rRNA (adenine-N6,N6-)-dimethyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables ribosomal small subunit binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within maturation of SSU-rRNA IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in methylation IEA
Inferred from Electronic Annotation
more info
  
involved_in rRNA base methylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in rRNA methylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in rRNA modification IEA
Inferred from Electronic Annotation
more info
  
involved_in rRNA processing IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within rRNA processing IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in response to antibiotic IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within ribosomal small subunit assembly IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of_or_within ribosomal small subunit assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
16S rRNA m(6)2A1518,m(6)2A1519 dimethyltransferase
NP_414593.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    51609..52430 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414593.1 16S rRNA m(6)2A1518,m(6)2A1519 dimethyltransferase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414593.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A6C9Q9Q7
    Conserved Domains (1) summary
    PRK00274
    Location:1 → 267
    ksgA; 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA
Nucleotide Protein
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