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pcm protein-L-isoaspartate O-methyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944923, updated on 26-Mar-2025
Official Symbol
pcm
Official Full Name
protein-L-isoaspartate O-methyltransferase
Primary source
ECOCYC:EG10689
Locus tag
b2743
See related
ASAP:ABE-0009006
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2738
Summary
Pcm may work with SurE: they are co-transcribed and have addictive effects on isoaspartyl accumulation during stationary phase. [More information is available at EcoGene: EG10689]. The pcm-encoded L-isoaspartate protein carboxylmethyltransferase serves to repair abnormal L-isoaspartyl residues that arise sponaneously from aspartyl and asparaginyl residues in proteins. [More information is available at EcoCyc: EG10689].
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Sequence:
NC_000913.3 (2868893..2869519, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene RNA polymerase sigma factor RpoS Neighboring gene murein hydrolase activator NlpD Neighboring gene broad specificity 5'(3')-nucleotidase and polyphosphatase Neighboring gene tRNA pseudouridine(13) synthase Neighboring gene 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase Neighboring gene 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. A protein methyltransferase specific for altered aspartyl residues is important in Escherichia coli stationary-phase survival and heat-shock resistance. Li C, et al. Proc Natl Acad Sci U S A, 1992 Oct 15. PMID 1409717, Free PMC Article
  2. Functional solubilization of aggregation-prone TRAIL protein facilitated by coexpressing with protein isoaspartate methyltranferase. Zhu H, et al. Appl Microbiol Biotechnol, 2006 Oct. PMID 16575568
  3. Purification, gene cloning, and sequence analysis of an L-isoaspartyl protein carboxyl methyltransferase from Escherichia coli. Fu JC, et al. J Biol Chem, 1991 Aug 5. PMID 1860862
  4. Recovery from long-term stationary phase and stress survival in Escherichia coli require the L-isoaspartyl protein carboxyl methyltransferase at alkaline pH. Hicks WM, et al. Microbiology (Reading), 2005 Jul. PMID 16000706
  5. Protein isoaspartate methyltransferase is a multicopy suppressor of protein aggregation in Escherichia coli. Kern R, et al. J Bacteriol, 2005 Feb. PMID 15687202, Free PMC Article

See all (19) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. N-terminus verified by Edman degradation on mature peptide
    Title: Purification, gene cloning, and sequence analysis of an L-isoaspartyl protein carboxyl methyltransferase from Escherichia coli.
  2. discovered that protein isoaspartate methyltransferase (PIMT) is a multicopy suppressor of preS2-S'-beta-galactosidase aggregation at 43 degrees C.
    Title: Protein isoaspartate methyltransferase is a multicopy suppressor of protein aggregation in Escherichia coli.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein-L-isoaspartate (D-aspartate) O-methyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein-L-isoaspartate (D-aspartate) O-methyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein-L-isoaspartate (D-aspartate) O-methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in methylation IEA
Inferred from Electronic Annotation
more info
  
involved_in protein modification process IEA
Inferred from Electronic Annotation
more info
  
involved_in protein repair IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within protein repair IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
protein-L-isoaspartate O-methyltransferase
NP_417223.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2868893..2869519 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417223.1 protein-L-isoaspartate O-methyltransferase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417223.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    Q4JH73
    Conserved Domains (1) summary
    PRK00312
    Location:1 → 208
    pcm; protein-L-isoaspartate(D-aspartate) O-methyltransferase
Nucleotide Protein
Heading Accession and Version

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