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queA tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944905, updated on 19-Sep-2024

Summary

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Official Symbol
queA
Official Full Name
tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase
Primary source
ECOCYC:EG10812
Locus tag
b0405
See related
ASAP:ABE-0001411
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0399; tsaA
Summary
Asn, Asp, Tyr, and His tRNA anticodons are modified. [More information is available at EcoGene: EG10812]. The queA gene encodes an S-adenosylmethionine:tRNA ribosyltransferase-isomerase, which catalyzes formation of the 2,3-epoxy-4,5-dihydroxycyclopentane ring of epoxyqueuosine (oQ), a precursor of the queuosine (Q) anticodon loop modification in tRNA(Asp), tRNA(Asn), tRNA(His), and tRNA(Tyr) . [More information is available at EcoCyc: EG10812].
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Genomic context

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Sequence:
NC_000913.3 (425011..426081)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene maltodextrin glucosidase Neighboring gene acyl carrier protein phosphodiesterase Neighboring gene tRNA-guanine transglycosylase Neighboring gene Sec translocon accessory complex subunit YajC

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. tRNA modification by S-adenosylmethionine:tRNA ribosyltransferase-isomerase. Assay development and characterization of the recombinant enzyme. Van Lanen SG, et al. J Biol Chem, 2003 Mar 21. PMID 12533518
  2. Mechanistic studies of the tRNA-modifying enzyme QueA: a chemical imperative for the use of AdoMet as a "ribosyl" donor. Kinzie SD, et al. Org Lett, 2000 May 4. PMID 10810734
  3. Screening orthologs as an important variable in crystallization: preliminary X-ray diffraction studies of the tRNA-modifying enzyme S-adenosyl-methionine:tRNA ribosyl transferase/isomerase. Grimm C, et al. Acta Crystallogr D Biol Crystallogr, 2000 Apr. PMID 10739928
  4. New function of vitamin B12: cobamide-dependent reduction of epoxyqueuosine to queuosine in tRNAs of Escherichia coli and Salmonella typhimurium. Frey B, et al. J Bacteriol, 1988 May. PMID 3129401, Free PMC Article
  5. Isolation and structure elucidation of an epoxide derivative of the hypermodified nucleoside queuosine from Escherichia coli transfer RNA. Phillipson DW, et al. J Biol Chem, 1987 Mar 15. PMID 3102486

See all (29) citations in PubMed

GeneRIFs: Gene References Into Functions

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables S-adenosylmethionine:tRNA ribosyltransferase-isomerase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables S-adenosylmethionine:tRNA ribosyltransferase-isomerase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables S-adenosylmethionine:tRNA ribosyltransferase-isomerase activity IEA
Inferred from Electronic Annotation
more info
  
enables isomerase activity IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in queuosine biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in queuosine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within queuosine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within response to radiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in tRNA modification IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA wobble guanine modification IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within tRNA wobble guanine modification IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase
NP_414939.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    425011..426081
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414939.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414939.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A826PG39
    Conserved Domains (1) summary
    PRK00147
    Location:1 → 344
    queA; S-adenosylmethionine:tRNA ribosyltransferase-isomerase; Provisional
Nucleotide Protein
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