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rseP intramembrane zinc metalloprotease RseP [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944871, updated on 30-Jul-2025
Official Symbol
rseP
Official Full Name
intramembrane zinc metalloprotease RseP
Primary source
ECOCYC:EG12436
Locus tag
b0176
See related
ASAP:ABE-0000603
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ecfE; ECK0175; yaeL
Summary
RpoE regulon. [More information is available at EcoGene: EG12436]. YaeL is a protease that resides in the inner membrane . [More information is available at EcoCyc: EG12436].
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Sequence:
NC_000913.3 (196546..197898)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene ditrans,polycis-undecaprenyl-diphosphate synthase Neighboring gene CDP-diglyceride synthetase Neighboring gene outer membrane protein assembly factor BamA Neighboring gene periplasmic chaperone Skp

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Biochemical Characterization of Function and Structure of RseP, an Escherichia coli S2P Protease. Hizukuri Y, et al. Methods Enzymol, 2017. PMID 28065260
  2. Roles of the membrane-reentrant β-hairpin-like loop of RseP protease in selective substrate cleavage. Akiyama K, et al. Elife, 2015 Oct 8. PMID 26447507, Free PMC Article
  3. A family of membrane-embedded metalloproteases involved in regulated proteolysis of membrane-associated transcription factors. Rudner DZ, et al. Proc Natl Acad Sci U S A, 1999 Dec 21. PMID 10611287, Free PMC Article
  4. Post-liberation cleavage of signal peptides is catalyzed by the site-2 protease (S2P) in bacteria. Saito A, et al. Proc Natl Acad Sci U S A, 2011 Aug 16. PMID 21810987, Free PMC Article
  5. Regulated intramembrane proteolysis: a control mechanism conserved from bacteria to humans. Brown MS, et al. Cell, 2000 Feb 18. PMID 10693756

See all (39) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Instability of CII is needed for efficient switching between lytic and lysogenic development in bacteriophage 186.
    Title: Instability of CII is needed for efficient switching between lytic and lysogenic development in bacteriophage 186.
  2. we describe several methods for characterization of the proteolytic functions and structure of RseP mainly in vivo, including a proteolytic activity assay using model substrates, an in vitro analysis of cleavage of signal peptides in a detergent solution and in the membrane vesicles, structural analysis of membrane-embedded RseP based on the thiol modifiability of introduced cysteine residues, and the protein interaction
    Title: Biochemical Characterization of Function and Structure of RseP, an Escherichia coli S2P Protease.
  3. Mutations disturbing the possible beta-strand conformation of the loop impaired RseP proteolytic activity and that some of these mutations resulted in the differential cleavage of different substrates.
    Title: Roles of the membrane-reentrant β-hairpin-like loop of RseP protease in selective substrate cleavage.
  4. study concludes that recognition of the cleaved amino acid by the RseP PDZ domain is not essential for sequential cleavage of RseA and sigma(E) stress response in vivo
    Title: PDZ domains of RseP are not essential for sequential cleavage of RseA or stress-induced σ(E) activation in vivo.
  5. Escherichia coli RseP, an site-2 protease (S2P) family I-CLiP, introduces a cleavage into signal peptides after their signal peptidase-mediated liberation from preproteins
    Title: Post-liberation cleavage of signal peptides is catalyzed by the site-2 protease (S2P) in bacteria.
  6. after DegS cleavage, the newly exposed carboxyl terminus of RseA may facilitate Site-2 cleavage through direct interaction with the PDZ domain.
    Title: Cleavage of RseA by RseP requires a carboxyl-terminal hydrophobic amino acid following DegS cleavage.
  7. circularly permutated PDZ domains control RseP, the S2P family intramembrane protease of Escherichia coli
    Title: A pair of circularly permutated PDZ domains control RseP, the S2P family intramembrane protease of Escherichia coli.
  8. RseP catalyzes proteolytic cleavage of the membrane-bound anti-sigma(E) protein RseA as an essential step in transmembrane signal transduction in the sigma(E) extracytoplasmic stress response pathway.
    Title: Substrate recognition and binding by RseP, an Escherichia coli intramembrane protease.
  9. RseP, which is required for normal sigmaE activation, prevents toxicity due to the presence of two specific outer membrane proteins that are down-regulated by RseX
    Title: Down-regulation of porins by a small RNA bypasses the essentiality of the regulated intramembrane proteolysis protease RseP in Escherichia coli.
Submit: New GeneRIF Correction

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables anti-sigma factor antagonist activity EXP
Inferred from Experiment
more info
 PubMed 
enables anti-sigma factor antagonist activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables anti-sigma factor antagonist activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables endopeptidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables endopeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metalloendopeptidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables metalloendopeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables metallopeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables peptidase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within cellular response to cell envelope stress IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in cellular response to stress IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within positive regulation of DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in proteolysis IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
intramembrane zinc metalloprotease RseP

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    196546..197898
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414718.1 intramembrane zinc metalloprotease RseP

    See identical proteins and their annotated locations for NP_414718.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A8S7EC66
    Conserved Domains (1) summary
    PRK10779
    Location:2 → 450
    PRK10779; sigma E protease regulator RseP
Nucleotide Protein
Heading Accession and Version

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