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rnhB RNase HII [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944852, updated on 23-Apr-2026
Official Symbol
rnhB
Official Full Name
RNase HII
Primary source
ECOCYC:EG10861
Locus tag
b0183
See related
ASAP:ABE-0000622
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0182
Summary
RNase HII cleaves RNA in RNA-DNA hybrids . [More information is available at EcoCyc: EG10861].
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Sequence:
NC_000913.3 (204493..205089)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 3-hydroxy-acyl- Neighboring gene acyl- Neighboring gene lipid A disaccharide synthase Neighboring gene DNA polymerase III subunit alpha Neighboring gene acetyl-CoA carboxyltransferase subunit alpha

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Mechanisms employed by Escherichia coli to prevent ribonucleotide incorporation into genomic DNA by Pol V. McDonald JP, et al. PLoS Genet, 2012. PMID 23144626, Free PMC Article
  2. The pH-dependence of the Escherichia coli RNase HII-catalysed reaction suggests that an active site carboxylate group participates directly in catalysis. Bastock JA, et al. J Mol Biol, 2007 Apr 27. PMID 17355881
  3. Isolation and characterization of a second RNase H (RNase HII) of Escherichia coli K-12 encoded by the rnhB gene. Itaya M. Proc Natl Acad Sci U S A, 1990 Nov. PMID 2172991, Free PMC Article
  4. RNase HII Saves rnhA Mutant Escherichia coli from R-Loop-Associated Chromosomal Fragmentation. Kouzminova EA, et al. J Mol Biol, 2017 Sep 15. PMID 28821455, Free PMC Article
  5. Excision of misincorporated ribonucleotides in DNA by RNase H (type 2) and FEN-1 in cell-free extracts. Rydberg B, et al. Proc Natl Acad Sci U S A, 2002 Dec 24. PMID 12475934, Free PMC Article

See all (15) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The authors propose that RNase H-deficient mutants convert some R-loops into R-tracts, which progress into R-gaps and then to double-strand breaks-explaining why R-tracts do not accumulate in RNase H-deficient cells, while double-strand breaks do.
    Title: RNase HII Saves rnhA Mutant Escherichia coli from R-Loop-Associated Chromosomal Fragmentation.
  2. N-terminus verified by Edman degradation on complete protein
    Title: Isolation and characterization of a second RNase H (RNase HII) of Escherichia coli K-12 encoded by the rnhB gene.
  3. The data suggest a requirement for ionisation of an active site carboxylic acid for metal ion binding or correct positioning of metal ion(s) in the enzyme-substrate complex and a role for a second active site carboxylate in general base catalysis.
    Title: The pH-dependence of the Escherichia coli RNase HII-catalysed reaction suggests that an active site carboxylate group participates directly in catalysis.
Submit: New GeneRIF Correction

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables RNA-DNA hybrid ribonuclease activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables RNA-DNA hybrid ribonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables RNA-DNA hybrid ribonuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables manganese ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleic acid binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in RNA catabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within ribonucleotide excision repair EXP
Inferred from Experiment
more info
 PubMed 
involved_in ribonucleotide excision repair IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
part_of ribonuclease H2 complex IBA
Inferred from Biological aspect of Ancestor
more info
  
Preferred Names
RNase HII
NP_414725.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    204493..205089
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414725.1 RNase HII

    See identical proteins and their annotated locations for NP_414725.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A831DSJ5, A0A8S7IDK3, A0A8S7XSV8
    Conserved Domains (1) summary
    PRK00015
    Location:10 → 190
    rnhB; ribonuclease HII; Validated
Nucleotide Protein
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