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gluQ [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944846, updated on 11-Sep-2024

Summary

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Official Symbol
gluQ
Primary source
ECOCYC:EG11362
Locus tag
b0144
See related
ASAP:ABE-0000497
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0143; yadB
Summary
A downstream start site at M10 is also possible; both starts have been used to overproduce active protein (Dubois, 2004; Campanacci, 2004). [More information is available at EcoGene: EG11362]. Glutamyl-Q tRNA(Asp) synthetase (Glu-Q-RS) catalyzes the addition of a glutamate residue to the queosine-modified wobble base of tRNA(Asp). [More information is available at EcoCyc: EG11362].
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Genomic context

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Sequence:
NC_000913.3 (159186..160112, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative fimbrial protein YadN Neighboring gene 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase Neighboring gene poly(A) polymerase I Neighboring gene RNA polymerase-binding transcription factor DksA Neighboring gene sugar fermentation stimulation protein A

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Critical role of zinc ion on E. coli glutamyl-queuosine-tRNA(Asp) synthetase (Glu-Q-RS) structure and function. Ray S, et al. Protein J, 2014 Apr. PMID 24505021
  2. Fusion with anticodon binding domain of GluRS is not sufficient to alter the substrate specificity of a chimeric Glu-Q-RS. Ray S, et al. Protein J, 2014 Feb. PMID 24374508
  3. Crystal structure of glutamyl-queuosine tRNAAsp synthetase complexed with L-glutamate: structural elements mediating tRNA-independent activation of glutamate and glutamylation of tRNAAsp anticodon. Blaise M, et al. J Mol Biol, 2008 Sep 19. PMID 18602926
  4. Turning tRNA upside down: When aminoacylation is not a prerequisite to protein synthesis. Ibba M, et al. Proc Natl Acad Sci U S A, 2004 May 18. PMID 15138304, Free PMC Article
  5. The Escherichia coli YadB gene product reveals a novel aminoacyl-tRNA synthetase like activity. Campanacci V, et al. J Mol Biol, 2004 Mar 19. PMID 15003446

See all (15) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The chimeric Glu-Q-RS is still able to aminoacylate tRNA(Asp) but has also the capacity to bind tRNA(Glu).
    Title: Fusion with anticodon binding domain of GluRS is not sufficient to alter the substrate specificity of a chimeric Glu-Q-RS.
Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables aminoacyl-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables glutamate-tRNA ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
Process Evidence Code Pubs
involved_in glutamyl-tRNA aminoacylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in glutamyl-tRNA aminoacylation IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA modification IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA wobble base modification IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
glutamyl-Q tRNA(Asp) synthetase

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    159186..160112 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414686.3 glutamyl-Q tRNA(Asp) synthetase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414686.3

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A066Q893, A0A0E0Y597, A0A2I6LMI9, A0A376U2V8, A0A7U9J0U7, A0A8E0FKH7, A0AA35F450, A0AAD2V788, A0AAD2VG64, A0AAI8HEX5, A0AAJ4DC63, B7LGK6, E0IYN7
    Conserved Domains (1) summary
    PRK05710
    Location:11 → 303
    PRK05710; tRNA glutamyl-Q(34) synthetase GluQRS
Nucleotide Protein
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