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hybA hydrogenase 2 [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944842, updated on 30-Jul-2025
Official Symbol
hybA
Official Full Name
hydrogenase 2
Primary source
ECOCYC:EG11799
Locus tag
b2996
See related
ASAP:ABE-0009832
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2990; hydL
Summary
Upregulated in biofilms; biofilm microarray result was validated with RT-PCR. [More information is available at EcoGene: EG11799]. The hybA-encoded protein may be involved in the periplasmic electron-transferring activity of hydrogenase 2 during catalytic turnover . [More information is available at EcoCyc: EG11799].
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Sequence:
NC_000913.3 (3144154..3145140, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene hydrogenase maturation protein HybF Neighboring gene hydrogenase 2-specific chaperone Neighboring gene hydrogenase 2 maturation protease Neighboring gene hydrogenase 2 large subunit Neighboring gene hydrogenase 2 membrane subunit Neighboring gene hydrogenase 2 small subunit Neighboring gene DUF2623 domain-containing protein YghW

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Partial characterization of an electrophoretically labile hydrogenase activity of Escherichia coli K-12. Stoker K, et al. J Bacteriol, 1988 Mar. PMID 3277948, Free PMC Article
  2. Isolation and characterization of mutant strains of Escherichia coli altered in H2 metabolism. Lee JH, et al. J Bacteriol, 1985 Apr. PMID 3884594, Free PMC Article
  3. Proton translocation coupled to formate oxidation in anaerobically grown fermenting Escherichia coli. Hakobyan M, et al. Biophys Chem, 2005 May 1. PMID 15848284
  4. Isolation and characterisation of a soluble active fragment of hydrogenase isoenzyme 2 from the membranes of anaerobically grown Escherichia coli. Ballantine SP, et al. Eur J Biochem, 1986 Apr 15. PMID 3516690
  5. Requirement for nickel of the transmembrane translocation of NiFe-hydrogenase 2 in Escherichia coli. Rodrigue A, et al. FEBS Lett, 1996 Aug 26. PMID 8772179

See all (27) citations in PubMed

GeneRIFs: Gene References Into Functions

Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 4 iron, 4 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables formate dehydrogenase (quinone) activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables hydrogen:quinone oxidoreductase activity EXP
Inferred from Experiment
more info
 PubMed 
enables iron-sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables iron-sulfur cluster binding RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within anaerobic glycerol catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within anaerobic respiration EXP
Inferred from Experiment
more info
 PubMed 
involved_in anaerobic respiration IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in anaerobic respiration IEA
Inferred from Electronic Annotation
more info
  
involved_in formate oxidation IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
part_of formate dehydrogenase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in periplasmic space IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane ISM
Inferred from Sequence Model
more info
 PubMed 
Preferred Names
hydrogenase 2
NP_417470.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3144154..3145140 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417470.1 hydrogenase 2 [Fe-S] protein

    See identical proteins and their annotated locations for NP_417470.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A1V2GF22
    Conserved Domains (1) summary
    PRK10882
    Location:1 → 328
    PRK10882; hydrogenase 2 operon protein HybA
Nucleotide Protein
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