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panB 3-methyl-2-oxobutanoate hydroxymethyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944839, updated on 26-Mar-2025
Official Symbol
panB
Official Full Name
3-methyl-2-oxobutanoate hydroxymethyltransferase
Primary source
ECOCYC:EG11675
Locus tag
b0134
See related
ASAP:ABE-0000467
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0133
Summary
3-methyl-2-oxobutanoate hydroxymethyltransferase (KPHMT) catalyzes the first committed step of the pantothenate biosynthesis pathway, transferring the C11 carbon of 5,10-methylene-tetrahydrofolate onto 2-keto-isovalerate to form 2-dehydropantoate . [More information is available at EcoCyc: EG11675].
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Sequence:
NC_000913.3 (148807..149601, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene recombination-promoting nuclease RpnC Neighboring gene pantothenate synthetase Neighboring gene fimbrial tip-adhesin YadC Neighboring gene putative fimbrial protein YadK

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Structure of E. coli ketopantoate hydroxymethyl transferase complexed with ketopantoate and Mg2+, solved by locating 160 selenomethionine sites. von Delft F, et al. Structure, 2003 Aug. PMID 12906829
  2. Comparative analysis of the Escherichia coli ketopantoate hydroxymethyltransferase crystal structure confirms that it is a member of the (betaalpha)8 phosphoenolpyruvate/pyruvate superfamily. Schmitzberger F, et al. J Bacteriol, 2003 Jul. PMID 12837791, Free PMC Article
  3. Isolation and characterization of Escherichia coli pantothenate permease (panF) mutants. Vallari DS, et al. J Bacteriol, 1985 Oct. PMID 2995306, Free PMC Article
  4. Genetic and biochemical analyses of pantothenate biosynthesis in Escherichia coli and Salmonella typhimurium. Cronan JE Jr, et al. J Bacteriol, 1982 Mar. PMID 7037743, Free PMC Article
  5. Assembly reflects evolution of protein complexes. Levy ED, et al. Nature, 2008 Jun 26. PMID 18563089, Free PMC Article

See all (21) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. N-terminus verified by Edman degradation on complete protein
    Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 3-methyl-2-oxobutanoate hydroxymethyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 3-methyl-2-oxobutanoate hydroxymethyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 3-methyl-2-oxobutanoate hydroxymethyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables magnesium ion binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in pantothenate biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in pantothenate biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within pantothenate biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol HDA PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane HDA PubMed 
Preferred Names
3-methyl-2-oxobutanoate hydroxymethyltransferase
NP_414676.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    148807..149601 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414676.1 3-methyl-2-oxobutanoate hydroxymethyltransferase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414676.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8S0G0N6
    Conserved Domains (1) summary
    PRK00311
    Location:3 → 264
    panB; 3-methyl-2-oxobutanoate hydroxymethyltransferase; Reviewed
Nucleotide Protein
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