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aceK isocitrate dehydrogenase kinase/phosphatase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944797, updated on 26-Mar-2025

Summary

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Official Symbol
aceK
Official Full Name
isocitrate dehydrogenase kinase/phosphatase
Primary source
ECOCYC:EG10026
Locus tag
b4016
See related
ASAP:ABE-0013131
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4008
Summary
Context determines which enzyme name is used, the kinase or the phosphatase. [More information is available at EcoCyc: EG10026].
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Genomic context

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Sequence:
NC_000913.3 (4218596..4220332)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene malate synthase A Neighboring gene isocitrate lyase Neighboring gene ncRNA Neighboring gene regulator of acetyl CoA synthetase Neighboring gene DNA-binding transcriptional repressor IclR Neighboring gene cobalamin-dependent methionine synthase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Photoaffinity labelling shows that Escherichia coli isocitrate dehydrogenase kinase/phosphatase contains a single ATP-binding site. Varela I, et al. FEBS Lett, 1988 Apr 25. PMID 2834232
  2. Isocitrate dehydrogenase kinase/phosphatase. Kinetic characteristics of the wild-type and two mutant proteins. Miller SP, et al. J Biol Chem, 1996 Aug 9. PMID 8702587
  3. Escherichia coli isocitrate dehydrogenase kinase/phosphatase. Overproduction and kinetics of interaction with its substrates by using intrinsic fluorescence and fluorescent nucleotide analogues. Rittinger K, et al. Eur J Biochem, 1996 Apr 1. PMID 8620880
  4. The isocitrate dehydrogenase phosphorylation cycle: regulation and enzymology. LaPorte DC. J Cell Biochem, 1993 Jan. PMID 8381789
  5. Determination of flux through the branch point of two metabolic cycles. The tricarboxylic acid cycle and the glyoxylate shunt. Walsh K, et al. J Biol Chem, 1984 Aug 10. PMID 6378912

See all (50) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Characterization of metal binding of bifunctional kinase/phosphatase AceK and implication in activity modulation.
    Title: Characterization of metal binding of bifunctional kinase/phosphatase AceK and implication in activity modulation.
  2. highly stringent AceK binding sites on isocitrate dehydrogenase
    Title: Structural basis of the substrate specificity of bifunctional isocitrate dehydrogenase kinase/phosphatase.
  3. In this study, AceK was successfully cocrystallized in complex with its intact protein substrate, isocitrate dehydrogenase, in the presence of ATP.
    Title: Purification, crystallization and preliminary X-ray analysis of bifunctional isocitrate dehydrogenase kinase/phosphatase in complex with its substrate, isocitrate dehydrogenase, from Escherichia coli.
  4. N-terminus verified by Edman degradation on mature peptide
    Title: Nucleotide sequence of aceK, the gene encoding isocitrate dehydrogenase kinase/phosphatase.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables AMP binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables AMP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ATP binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ATP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables [isocitrate dehydrogenase (NADP+)] kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables [isocitrate dehydrogenase (NADP+)] kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables [isocitrate dehydrogenase (NADP+)] kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, acting on ester bonds IEA
Inferred from Electronic Annotation
more info
  
enables kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables manganese ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables phosphoprotein phosphatase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables phosphoprotein phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein serine/threonine kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein serine/threonine kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein serine/threonine phosphatase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in glucose metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within glyoxylate cycle IC
Inferred by Curator
more info
 PubMed 
involved_in glyoxylate cycle IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within tricarboxylic acid cycle IC
Inferred by Curator
more info
 PubMed 
involved_in tricarboxylic acid cycle IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IC
Inferred by Curator
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
isocitrate dehydrogenase kinase/phosphatase
NP_418440.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4218596..4220332
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418440.1 isocitrate dehydrogenase kinase/phosphatase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418440.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A376FSU2, A0A3K1A8W6
    Conserved Domains (1) summary
    COG4579
    Location:1 → 578
    AceK; Isocitrate dehydrogenase kinase/phosphatase [Signal transduction mechanisms]
Nucleotide Protein
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