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aceF pyruvate dehydrogenase E2 subunit [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944794, updated on 4-Feb-2026
Official Symbol
aceF
Official Full Name
pyruvate dehydrogenase E2 subunit
Primary source
ECOCYC:EG10025
Locus tag
b0115
See related
ASAP:ABE-0000400
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0114
Summary
Holoenzyme is AceE(24)+AceF(24)+Lpd(12). [More information is available at EcoGene: EG10025]. AceF, the "E2" or "core" component of the pyruvate dehydrogenase multienzyme complex, assembles into a 24-subunit cube . [More information is available at EcoCyc: EG10025].
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Sequence:
NC_000913.3 (125695..127587)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-binding transcriptional dual regulator PdhR Neighboring gene pyruvate dehydrogenase E1 component Neighboring gene lipoamide dehydrogenase Neighboring gene DUF3300 domain-containing protein YacH

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Isolation and characterization of lipoylated and unlipoylated domains of the E2p subunit of the pyruvate dehydrogenase complex of Escherichia coli. Ali ST, et al. Biochem J, 1990 Oct 1. PMID 2121129, Free PMC Article
  2. Restricted motion of the lipoyl-lysine swinging arm in the pyruvate dehydrogenase complex of Escherichia coli. Jones DD, et al. Biochemistry, 2000 Jul 25. PMID 10913250
  3. Identification of the E2-binding residues in the N-terminal domain of E1 of a prokaryotic pyruvate dehydrogenase complex. Hengeveld AF, et al. FEBS Lett, 2002 Jul 3. PMID 12095640
  4. Histidine 407, a phantom residue in the E1 subunit of the Escherichia coli pyruvate dehydrogenase complex, activates reductive acetylation of lipoamide on the E2 subunit. An explanation for conservation of active sites between the E1 subunit and transketolase. Nemeria N, et al. Biochemistry, 2002 Dec 31. PMID 12501174
  5. Primary biliary cirrhosis: an orchestrated immune response against epithelial cells. Gershwin ME, et al. Immunol Rev, 2000 Apr. PMID 10807518

See all (88) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Dimerization of a 5-kDa domain defines the architecture of the 5-MDa gammaproteobacterial pyruvate dehydrogenase complex.
    Title: Dimerization of a 5-kDa domain defines the architecture of the 5-MDa gammaproteobacterial pyruvate dehydrogenase complex.
  2. The inclusion of each additional domain of E2p strengthened the interaction with E1p, and the interaction was strongest with intact E2p.
    Title: Structure and function of the catalytic domain of the dihydrolipoyl acetyltransferase component in Escherichia coli pyruvate dehydrogenase complex.
  3. The surface loop of E1o is important to the structural integrity of the protein and together with Thr42 plays an important role in specifying the interaction of the lipoyl domain with its partner E1o in the E. coli 2OGDH complex.
    Title: The role of loop and beta-turn residues as structural and functional determinants for the lipoyl domain from the Escherichia coli 2-oxoglutarate dehydrogenase complex.
  4. N-terminus verified by Edman degradation on mature peptide
    Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
  5. the dynamic loop at the active center of the Escherichia coli pyruvate dehydrogenase complex E1 component modulates substrate utilization and chemical communication with the E2 component
    Title: A dynamic loop at the active center of the Escherichia coli pyruvate dehydrogenase complex E1 component modulates substrate utilization and chemical communication with the E2 component.
  6. Pyruvate dehydrogenase complex E2 (PDHc-E2)subunit protects N-terminal residues 1-55 of PDHc-E1 subunit selectively from trypsin digestion.
    Title: Amino-terminal residues 1-45 of the Escherichia coli pyruvate dehydrogenase complex E1 subunit interact with the E2 subunit and are required for activity of the complex but not for reductive acetylation of the E2 subunit.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables acyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables dihydrolipoyllysine-residue acetyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables dihydrolipoyllysine-residue acetyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables dihydrolipoyllysine-residue acetyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables lipoic acid binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in pyruvate catabolic process NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in pyruvate decarboxylation to acetyl-CoA IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within pyruvate decarboxylation to acetyl-CoA IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within stress response to acid chemical IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm HDA PubMed 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
part_of pyruvate dehydrogenase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of pyruvate dehydrogenase complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of pyruvate dehydrogenase complex IEA
Inferred from Electronic Annotation
more info
  
part_of pyruvate dehydrogenase complex IGI
Inferred from Genetic Interaction
more info
 PubMed 
Preferred Names
pyruvate dehydrogenase E2 subunit
NP_414657.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    125695..127587
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414657.1 pyruvate dehydrogenase E2 subunit

    See identical proteins and their annotated locations for NP_414657.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A5N8H5Z5
    Conserved Domains (1) summary
    PRK11854
    Location:1 → 630
    aceF; pyruvate dehydrogenase dihydrolipoyltransacetylase; Validated
Nucleotide Protein
Heading Accession and Version

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