U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

araC DNA-binding transcriptional dual regulator AraC [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944780, updated on 17-Dec-2024
Official Symbol
araC
Official Full Name
DNA-binding transcriptional dual regulator AraC
Primary source
ECOCYC:EG10054
Locus tag
b0064
See related
ASAP:ABE-0000236
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0065
Summary
AraC activates transcrption for araE and the araBAD and araFGH operons; AraC transcriptional activator family prototype. [More information is available at EcoGene: EG10054]. The "arabinose regulator," AraC, is a transcription factor that regulates transcription of several genes and operons involved in arabinose catabolism and transport. [More information is available at EcoCyc: EG10054].
NEW
Try the new Gene table
Try the new Transcript table
Sequence:
NC_000913.3 (70387..71265)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene L-arabinose isomerase Neighboring gene ribulokinase Neighboring gene DedA family protein YabI Neighboring gene thiamine ABC transporter ATP binding subunit Neighboring gene thiamine ABC transporter membrane subunit

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Transcription start site and induction kinetics of the araC regulatory gene in Escherichia coli K-12. Stoner CM, et al. J Mol Biol, 1983 Nov 15. PMID 6196488
  2. The interaction of L-arabinose and D-fucose with AraC protein. Wilcox G. J Biol Chem, 1974 Nov 10. PMID 4608387
  3. Direction of transcription of the regulatory gene araC in Escherichia coli B-r. Wilcox G, et al. Proc Natl Acad Sci U S A, 1974 Sep. PMID 4610582, Free PMC Article
  4. Isolation of araC protein by affinity chromatography. Wilcox G, et al. Methods Enzymol, 1974. PMID 4615240
  5. Nonsense mutants in the regulator gene araC of the L-arabinose system of Escherichia coli B-r. Irr J, et al. Genetics, 1970 May. PMID 4920896, Free PMC Article

See all (119) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Atomic force microscopy measurements and model of DNA bending caused by binding of AraC protein.
    Title: Atomic force microscopy measurements and model of DNA bending caused by binding of AraC protein.
  2. Allosteric regulation within the highly interconnected structural scaffold of AraC/XylS homologs tolerates a wide range of amino acid changes.
    Title: Allosteric regulation within the highly interconnected structural scaffold of AraC/XylS homologs tolerates a wide range of amino acid changes.
  3. Engineering AraC to make it responsive to light instead of arabinose.
    Title: Engineering AraC to make it responsive to light instead of arabinose.
  4. Arabinose binding to the AraC dimerization domain changes the properties of the AraC inter-domain linker which connects the dimerization domain to the DNA binding domain via interactions that do not depend on the DNA binding domain.
    Title: A genetic and physical study of the interdomain linker of E. Coli AraC protein--a trans-subunit communication pathway.
  5. Data [including data from studies of mutant AraC (P8G, P8A)] suggest that, in AraC conformation, interaction of the N-terminal arm with something other than the front lip is the primary determinant of the inducing versus repressing state of AraC.
    Title: Computational and experimental investigation of constitutive behavior in AraC.
  6. Interaction between domains of AraC in presence of arabinose is reported. Dissociation of arabinose-bound AraC from half-site DNA is faster than free DNA binding domain;affinity of dimeriz'n domains for arabinose is increased when half-site DNA is bound.
    Title: A new and unexpected domain-domain interaction in the AraC protein.
  7. Mutation in the interdomain affect the dimerization domain-DNA-binding domain interactions in AraC.
    Title: Active role of the interdomain linker of AraC.
  8. The majority of the constitutive mutations lie within the same residues of the N-terminal regulatory arm of AraC. Two, however, were found in the core of the dimerization domain.
    Title: Constitutive mutations in the Escherichia coli AraC protein.
  9. N-terminus verified by Edman degradation on mature peptide
    Title: The araC gene of Escherichia coli: transcriptional and translational start-points and complete nucleotide sequence.
  10. The allosteric mechanism by which the gene expression regulatory protein AraC regulates its DNA-binding activity is shown to be portable.
    Title: A portable allosteric mechanism.
Submit: New GeneRIF Correction See all GeneRIFs (11)
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA-binding transcription factor activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA-binding transcription repressor activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables sequence-specific DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables transcription cis-regulatory region binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in arabinose catabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within arabinose catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of primary metabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of protein-DNA complex IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Preferred Names
DNA-binding transcriptional dual regulator AraC

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    70387..71265
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414606.1 DNA-binding transcriptional dual regulator AraC [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414606.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A6N0IPE6
    Conserved Domains (1) summary
    PRK10572
    Location:1 → 285
    PRK10572; arabinose operon transcriptional regulator AraC
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.