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ileS isoleucine--tRNA ligase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944761, updated on 26-Mar-2025
Official Symbol
ileS
Official Full Name
isoleucine--tRNA ligase
Primary source
ECOCYC:EG10492
Locus tag
b0026
See related
ASAP:ABE-0000094
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0027; ilvS
Summary
ileS is an essential gene; the ileS mutants in the Keio collection have a second intact copy of ileS (R. [More information is available at EcoGene: EG10492]. Isoleucyl-tRNA synthetase (IleRS) is a member of the family of aminoacyl-tRNA synthetases, which interpret the genetic code by covalently linking amino acids to their specific tRNA molecules. [More information is available at EcoCyc: EG10492].
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Sequence:
NC_000913.3 (22391..25207)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF2575 domain-containing protein YaaY Neighboring gene bifunctional riboflavin kinase/FMN adenylyltransferase Neighboring gene lipoprotein signal peptidase Neighboring gene peptidyl-prolyl cis-trans isomerase FkpB

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Alternative pathways for editing non-cognate amino acids by aminoacyl-tRNA synthetases. Jakubowski H, et al. Nucleic Acids Res, 1981 Jul 10. PMID 7024910, Free PMC Article
  2. Isotope labeling of free and aminoacyl transfer RNA synthetase-bound transfer RNA. Schoemaker HJ, et al. J Biol Chem, 1976 Nov 10. PMID 789377
  3. Zinc-dependent tRNA binding by a peptide element within a tRNA synthetase. Glasfeld E, et al. Biochemistry, 1997 Jun 3. PMID 9184155
  4. C-terminal zinc-containing peptide required for RNA recognition by a class I tRNA synthetase. Glasfeld E, et al. Biochemistry, 1996 Apr 2. PMID 8672449
  5. Protein synthesis editing by a DNA aptamer. Hale SP, et al. Proc Natl Acad Sci U S A, 1996 Apr 2. PMID 8610114, Free PMC Article

See all (94) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Mechanism of discrimination of isoleucyl-tRNA synthetase against nonproteinogenic alpha-aminobutyrate and its fluorinated analogues.
    Title: Mechanism of discrimination of isoleucyl-tRNA synthetase against nonproteinogenic α-aminobutyrate and its fluorinated analogues.
  2. Data indicate that tyrosine 59 participates in the synthetic reaction of isoleucyl-tRNA synthetase (EcIleRS).
    Title: Determinants for tRNA-dependent pretransfer editing in the synthetic site of isoleucyl-tRNA synthetase.
  3. analysis of three models for pre-transfer editing by Escherichia coli isoleucyl-tRNA synthetase (IleRS)
    Title: Partitioning of tRNA-dependent editing between pre- and post-transfer pathways in class I aminoacyl-tRNA synthetases.
  4. catalytic flexibility of tRNAIle-lysidine synthetase can generate alternative tRNA substrates for isoleucyl-tRNA synthetase
    Title: The catalytic flexibility of tRNAIle-lysidine synthetase can generate alternative tRNA substrates for isoleucyl-tRNA synthetase.
  5. N-terminus verified by Edman degradation on mature peptide
    Title: Relationship of protein structure of isoleucyl-tRNA synthetase with pseudomonic acid resistance of Escherichia coli. A proposed mode of action of pseudomonic acid as an inhibitor of isoleucyl-tRNA synthetase.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables aminoacyl-tRNA deacylase activity IEA
Inferred from Electronic Annotation
more info
  
enables aminoacyl-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables isoleucine-tRNA ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables isoleucine-tRNA ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables isoleucine-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables tRNA binding IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in aminoacyl-tRNA metabolism involved in translational fidelity IEA
Inferred from Electronic Annotation
more info
  
involved_in isoleucyl-tRNA aminoacylation IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within isoleucyl-tRNA aminoacylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in isoleucyl-tRNA aminoacylation IEA
Inferred from Electronic Annotation
more info
  
involved_in response to antibiotic IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA aminoacylation for protein translation IEA
Inferred from Electronic Annotation
more info
  
involved_in translation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
isoleucine--tRNA ligase
NP_414567.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    22391..25207
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414567.1 isoleucine--tRNA ligase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414567.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A376MCN1
    Conserved Domains (1) summary
    PRK05743
    Location:1 → 930
    ileS; isoleucyl-tRNA synthetase; Reviewed
Nucleotide Protein
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