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AP1M1 adaptor related protein complex 1 subunit mu 1 [ Homo sapiens (human) ]

Gene ID: 8907, updated on 7-Jun-2020

Summary

Official Symbol
AP1M1provided by HGNC
Official Full Name
adaptor related protein complex 1 subunit mu 1provided by HGNC
Primary source
HGNC:HGNC:13667
See related
Ensembl:ENSG00000072958 MIM:603535
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
AP47; CLTNM; MU-1A; CLAPM2
Summary
The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in testis (RPKM 31.3), brain (RPKM 17.8) and 25 other tissues See more
Orthologs

Genomic context

See AP1M1 in Genome Data Viewer
Location:
19p13.11
Exon count:
13
Annotation release Status Assembly Chr Location
109.20200522 current GRCh38.p13 (GCF_000001405.39) 19 NC_000019.10 (16197911..16245906)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 19 NC_000019.9 (16308665..16346156)

Chromosome 19 - NC_000019.10Genomic Context describing neighboring genes Neighboring gene calcium and integrin binding family member 3 Neighboring gene family with sequence similarity 32 member A Neighboring gene Kruppel like factor 2 Neighboring gene epidermal growth factor receptor pathway substrate 15 like 1 Neighboring gene ribosomal protein S2 pseudogene 51

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp160, precursor env HIV-1 gp160 is identified to have a physical interaction with adaptor-related protein complex 1, mu 1 subunit (AP1M1) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed
env Two tyrosine-based endocytic signals (residues 704-710 and 760-766) in the cytosolic tail (gp41) of the HIV-1 envelope glycoprotein (Env) complex interact with members of the adaptor medium chain family, AP-1 mu1, AP-2 mu2 and AP-3 mu3A PubMed
Envelope transmembrane glycoprotein gp41 env The cytoplasmic domain (residues 707-856) of HIV-1 gp41 interacts with whole clathrin-associated AP-1 and AP-2 adaptor complexes PubMed
env The highly conserved C-terminal dileucine motif (residues 851-856) in the cytosolic domain of HIV-1 gp41 interacts with clathrin-associated AP-1 adaptor complexes PubMed
env Two tyrosine-based endocytic signals (residues 704-710 and 760-766) in the cytosolic tail (gp41) of the HIV-1 envelope glycoprotein (Env) complex interact with members of the adaptor medium chain family, AP-1 mu1, AP-2 mu2 and AP-3 mu3A PubMed
Nef nef HIV-1 Nef downregulates CD4 and MHC I by connecting these receptors with the endocytic machinery through direct interactions between Nef and the mu medium chains of AP complexes, essential components of clathrin-coated pits PubMed
nef Two distinct regions in Nef, from amino acids 140-150 and from 180-190, can both interact with the mu subunit of the adaptor protein complex 1 (AP-1) PubMed
nef A leucine-based motif near the C-terminus of HIV-1 Nef interacts with AP-1 complexes; residues 164 and 165 in Nef are required for the interaction with AP-1 PubMed
nef Adaptor-related protein complex 1 (AP-1) is necessary for cross-presentation by MHC-I HLA-A and HLA-B molecules containing a cytoplasmic tail tyrosine signal and that HIV-1 Nef inhibits the cross-presentation in antigen-presenting cells PubMed
nef Dominant active ARF1 (Q71L) potently stabilizes interactions among AP-1 mu1, HIV-1 Nef, and HLA-A2 and that the formation of a static complex sequesters necessary trafficking components PubMed
nef A quadruple mutation of mu 1 (K274E/K298E/K302E/R303D) abolishes binding to HIV-1 Nef, but a complementary mutation in the acidic cluster in Nef (62EEEE65 to 62KKKK65) restores their interaction PubMed
nef Asp327 and Tyr320 of MHC-I, Asp123 of Nef, and Arg225, Arg393, Lys396, Arg211, and Arg246 of mu 1 are involved in a crucial three-way electrostatic network, which results in the Nef-MHC-I CD-mu 1 complex formation PubMed
nef Exogenous Nef and TNF-alpha synergistically activate NF-kappaB and AP-1 resulting in enhancing viral replication in both chronically infected promonocytic cells and acutely infected primary macrophages PubMed
nef HIV-1 Nef acidic (Glu62-65) and polyproline domains (Pro75/78) stabilize the interaction between the HLA-A2/Nef fusion protein and AP-1 mu1 PubMed
nef The mu1 subunit of AP-1 uses its canonical tyrosine binding pocket for Nef-induced downregulation of HLA-A2. M20 is the only amino acid in the N-terminal domain of Nef needed for HLA-A2 downregulation PubMed
nef Mutating three amino acids (Y320, A324, and D327) in the cytoplasmic tail of HLA-A2 abrogates Nef-induced downregulation of HLA-A2 through a failuer to recruit the mu1 or gamma subunits of AP-1 PubMed
nef Knocking down either AP-1 gamma, AP-1 mu1, or clathrin strongly inhibits Nef-induced downregulation of HLA-A2 PubMed
nef A fusion protein, composed of the cytoplasmic domain (CD) of MHC-I and HIV-1 Nef, shows its binding to the mu subunit of AP-1, which requires the tyrosine residue of the YSQA sequence in the MHC-I CD and Nef residues E62-65 and P78 PubMed
nef HIV-1 Nef acts in cis to induce the rapid endocytosis of CD8-Nef fusion proteins via binding to the mu 1 chain of the adaptor protein type 1 (AP1) complex PubMed
nef HIV-1 Nef stabilizes AP-1 complexes on endosomal membranes after ADP-ribosylation factor-1 (ARF1) -dependent attachment PubMed
Pr55(Gag) gag HIV-1 Gag mutant lacking the matrix domain is insensitive to AP-1 mu depletion, suggesting that AP-1 mu participates in HIV-1 release through a direct interaction with the MA domain of Gag PubMed
gag HIV-1 Gag interacts with adaptor complex AP-1 mu in vitro. The release of HIV-1 Gag is inhibited by silencing AP-1 mu in cells PubMed
Vpu vpu HIV-1 Vpu physically interacts with the mu subunit of adaptor-related protein complex 1 (AP1) in cells, and Vpu R44 and L/I45 potentially interact with the mu 1 subunit PubMed
vpu Fusion of the Vpu cytoplasmic domain (residues 28-80) to the BST2 cytoplasmic domain (residues 1-21) enhances binding to the beta, mu, and sigma subunits of adaptor-related protein complex 1 (AP1) in cells PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
clathrin adaptor activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
Process Evidence Code Pubs
antigen processing and presentation of exogenous peptide antigen via MHC class II TAS
Traceable Author Statement
more info
 
endosome to melanosome transport IMP
Inferred from Mutant Phenotype
more info
PubMed 
intracellular protein transport IEA
Inferred from Electronic Annotation
more info
 
melanosome organization IMP
Inferred from Mutant Phenotype
more info
PubMed 
neutrophil degranulation TAS
Traceable Author Statement
more info
 
regulation of defense response to virus by virus TAS
Traceable Author Statement
more info
 
vesicle-mediated transport IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
Component Evidence Code Pubs
Golgi membrane TAS
Traceable Author Statement
more info
 
clathrin adaptor complex IEA
Inferred from Electronic Annotation
more info
 
clathrin-coated vesicle IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
clathrin-coated vesicle membrane IEA
Inferred from Electronic Annotation
more info
 
cytoplasmic vesicle IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
cytoplasmic vesicle membrane TAS
Traceable Author Statement
more info
 
cytosol TAS
Traceable Author Statement
more info
 
extracellular exosome HDA PubMed 
intracellular membrane-bounded organelle IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
lysosomal membrane TAS
Traceable Author Statement
more info
 
membrane HDA PubMed 
plasma membrane TAS
Traceable Author Statement
more info
 
specific granule membrane TAS
Traceable Author Statement
more info
 
trans-Golgi network membrane TAS
Traceable Author Statement
more info
 

General protein information

Preferred Names
AP-1 complex subunit mu-1
Names
AP-mu chain family member mu1A
HA1 47 kDa subunit
adapter-related protein complex 1 subunit mu-1
adaptor protein complex AP-1 mu-1 subunit
adaptor protein complex AP-1 subunit mu-1
adaptor related protein complex 1 mu 1 subunit
clathrin adaptor protein AP47
clathrin assembly protein complex 1 medium chain 1
clathrin assembly protein complex 1 mu-1 medium chain 1
clathrin assembly protein complex 1, medium chain
clathrin assembly protein complex AP1, mu subunit
clathrin coat assembly protein AP47
clathrin coat-associated protein AP47
golgi adaptor AP-1 47 kDa protein
golgi adaptor HA1/AP1 adaptin mu-1 subunit
mu-adaptin 1
mu1A-adaptin

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001130524.2NP_001123996.1  AP-1 complex subunit mu-1 isoform 1

    See identical proteins and their annotated locations for NP_001123996.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the longer isoform (1).
    Source sequence(s)
    AB209808, AC020911, BQ014582, BU729646, DC386056, DQ059565
    Consensus CDS
    CCDS46008.1
    UniProtKB/Swiss-Prot
    Q9BXS5
    UniProtKB/TrEMBL
    Q59EK3
    Related
    ENSP00000388996.1, ENST00000444449.6
    Conserved Domains (1) summary
    cd09258
    Location:153434
    AP-1_Mu1A_Cterm; C-terminal domain of medium Mu1A subunit in ubiquitously expressed clathrin-associated adaptor protein (AP) complex AP-1
  2. NM_032493.4NP_115882.1  AP-1 complex subunit mu-1 isoform 2

    See identical proteins and their annotated locations for NP_115882.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) lacks a coding exon in the middle region, as compared to variant 1. The reading frame is not affected and the resulting isoform (2) lacks an internal segment, as compared to isoform 1.
    Source sequence(s)
    AC020911, AK027528, BC017469, BQ014582, BU729646, DC386056
    Consensus CDS
    CCDS12342.1
    UniProtKB/Swiss-Prot
    Q9BXS5
    UniProtKB/TrEMBL
    A0A024R7K8, B3KNH5
    Related
    ENSP00000291439.2, ENST00000291439.7
    Conserved Domains (2) summary
    cd09258
    Location:153422
    AP-1_Mu1A_Cterm; C-terminal domain of medium Mu1A subunit in ubiquitously expressed clathrin-associated adaptor protein (AP) complex AP-1
    cd14835
    Location:4142
    AP1_Mu_N; AP-1 complex subunit mu N-terminal domain

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000019.10 Reference GRCh38.p13 Primary Assembly

    Range
    16197911..16245906
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
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