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SQSTM1 sequestosome 1 [ Homo sapiens (human) ]

Gene ID: 8878, updated on 20-Sep-2020

Summary

Official Symbol
SQSTM1provided by HGNC
Official Full Name
sequestosome 1provided by HGNC
Primary source
HGNC:HGNC:11280
See related
Ensembl:ENSG00000161011 MIM:601530
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3
Summary
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]
Expression
Ubiquitous expression in thyroid (RPKM 57.3), gall bladder (RPKM 54.3) and 25 other tissues See more
Orthologs

Genomic context

See SQSTM1 in Genome Data Viewer
Location:
5q35.3
Exon count:
11
Annotation release Status Assembly Chr Location
109.20200815 current GRCh38.p13 (GCF_000001405.39) 5 NC_000005.10 (179806393..179838078)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 5 NC_000005.9 (179233388..179265078)

Chromosome 5 - NC_000005.10Genomic Context describing neighboring genes Neighboring gene leukotriene C4 synthase Neighboring gene microRNA 1229 Neighboring gene alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B Neighboring gene Sharpr-MPRA regulatory region 15558 Neighboring gene MRN complex interacting protein Neighboring gene RN7SK pseudogene 150 Neighboring gene uncharacterized LOC100996419

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Replication interactions

Interaction Pubs
Treatment of macrophages (24-72 hours) with purified HIV-1 decreases SQSTM1 in a dose-dependent manner in the absence of cytotoxic effects (indicates induction and flux of autophagy)with observed increase is lost at day 7 post-infection onward PubMed

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env HIV-1 Env gp120 treatment of SH-SY5Y cells decreases SQSTM1 (p62) which indicates the progression of the autophagic process PubMed
Nef nef HIV-1 Nef upregulates the expression of p62 protein in human mesenchymal stem cells PubMed
Tat tat HIV-1 Tat downregulates sequestosome 1 (SQSTM1, p62) levels in a dose-dependent manner in neuronal cells PubMed
tat The interaction of SQSTM1/p62 with HIV-1 Tat is required for Tat degradation by the autophagy pathway in an ubiquitin-independent manner PubMed
Vif vif HIV-1 Vif is identified to have a physical interaction with sequestosome 1 (SQSTM1) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed
capsid gag HIV-1 CA colocalizes with MAP1LC3B2 and MAP1LC3B2/SQSTM1 as demonstrated via fluorescence microscopy PubMed
matrix gag HIV-1 p55/p17 (Gag-precursor) colocalizes with MAP1LC3B2 and MAP1LC3B2/SQSTM1 as demonstrated via fluorescence microscopy PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
K63-linked polyubiquitin modification-dependent protein binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
K63-linked polyubiquitin modification-dependent protein binding IDA
Inferred from Direct Assay
more info
PubMed 
SH2 domain binding IDA
Inferred from Direct Assay
more info
PubMed 
enzyme binding IPI
Inferred from Physical Interaction
more info
PubMed 
identical protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
ionotropic glutamate receptor binding ISS
Inferred from Sequence or Structural Similarity
more info
 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein kinase C binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
protein kinase C binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein kinase binding IDA
Inferred from Direct Assay
more info
PubMed 
protein serine/threonine kinase activity NAS
Non-traceable Author Statement
more info
PubMed 
protein-containing complex binding IEA
Inferred from Electronic Annotation
more info
 
receptor tyrosine kinase binding TAS
Traceable Author Statement
more info
PubMed 
ubiquitin binding IDA
Inferred from Direct Assay
more info
PubMed 
ubiquitin binding TAS
Traceable Author Statement
more info
 
ubiquitin protein ligase binding IDA
Inferred from Direct Assay
more info
PubMed 
ubiquitin protein ligase binding IPI
Inferred from Physical Interaction
more info
PubMed 
zinc ion binding IEA
Inferred from Electronic Annotation
more info
 
Process Evidence Code Pubs
aggrephagy IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
aggrephagy IPI
Inferred from Physical Interaction
more info
PubMed 
apoptotic process IEA
Inferred from Electronic Annotation
more info
 
autophagy IDA
Inferred from Direct Assay
more info
PubMed 
autophagy IMP
Inferred from Mutant Phenotype
more info
PubMed 
autophagy TAS
Traceable Author Statement
more info
PubMed 
autophagy of mitochondrion NAS
Non-traceable Author Statement
more info
PubMed 
cell differentiation IEA
Inferred from Electronic Annotation
more info
 
endosomal transport TAS
Traceable Author Statement
more info
PubMed 
endosome organization IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
endosome organization IDA
Inferred from Direct Assay
more info
PubMed 
immune system process IEA
Inferred from Electronic Annotation
more info
 
interleukin-1-mediated signaling pathway TAS
Traceable Author Statement
more info
 
intracellular signal transduction TAS
Traceable Author Statement
more info
PubMed 
macroautophagy IMP
Inferred from Mutant Phenotype
more info
PubMed 
macroautophagy ISS
Inferred from Sequence or Structural Similarity
more info
 
macroautophagy TAS
Traceable Author Statement
more info
 
mitochondrion organization IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
mitophagy IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
mitophagy IGI
Inferred from Genetic Interaction
more info
PubMed 
negative regulation of apoptotic process TAS
Traceable Author Statement
more info
 
negative regulation of protein ubiquitination IDA
Inferred from Direct Assay
more info
PubMed 
negative regulation of transcription by RNA polymerase II IEA
Inferred from Electronic Annotation
more info
 
positive regulation of apoptotic process TAS
Traceable Author Statement
more info
 
positive regulation of long-term synaptic potentiation ISS
Inferred from Sequence or Structural Similarity
more info
 
positive regulation of protein localization to plasma membrane ISS
Inferred from Sequence or Structural Similarity
more info
 
positive regulation of protein phosphorylation IEA
Inferred from Electronic Annotation
more info
 
positive regulation of transcription by RNA polymerase II TAS
Traceable Author Statement
more info
PubMed 
protein localization TAS
Traceable Author Statement
more info
PubMed 
protein localization to perinuclear region of cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
protein phosphorylation IEA
Inferred from Electronic Annotation
more info
 
regulation of I-kappaB kinase/NF-kappaB signaling IMP
Inferred from Mutant Phenotype
more info
PubMed 
regulation of Ras protein signal transduction NAS
Non-traceable Author Statement
more info
PubMed 
regulation of mitochondrion organization NAS
Non-traceable Author Statement
more info
PubMed 
regulation of protein complex stability IDA
Inferred from Direct Assay
more info
PubMed 
response to ischemia IEA
Inferred from Electronic Annotation
more info
 
response to mitochondrial depolarisation IGI
Inferred from Genetic Interaction
more info
PubMed 
selective autophagy IMP
Inferred from Mutant Phenotype
more info
PubMed 
ubiquitin-dependent protein catabolic process TAS
Traceable Author Statement
more info
PubMed 
Component Evidence Code Pubs
Lewy body IEA
Inferred from Electronic Annotation
more info
 
P-body IDA
Inferred from Direct Assay
more info
PubMed 
PML body IDA
Inferred from Direct Assay
more info
PubMed 
aggresome IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
amphisome IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
amphisome IDA
Inferred from Direct Assay
more info
PubMed 
autolysosome IDA
Inferred from Direct Assay
more info
PubMed 
autophagosome IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
autophagosome IDA
Inferred from Direct Assay
more info
PubMed 
cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
cytosol IDA
Inferred from Direct Assay
more info
 
cytosol TAS
Traceable Author Statement
more info
PubMed 
endoplasmic reticulum IEA
Inferred from Electronic Annotation
more info
 
extracellular exosome HDA PubMed 
inclusion body IDA
Inferred from Direct Assay
more info
PubMed 
intracellular membrane-bounded organelle IDA
Inferred from Direct Assay
more info
 
late endosome IEA
Inferred from Electronic Annotation
more info
 
mitochondrion IEA
Inferred from Electronic Annotation
more info
 
nucleoplasm TAS
Traceable Author Statement
more info
 
phagophore assembly site IEA
Inferred from Electronic Annotation
more info
 
sarcomere IEA
Inferred from Electronic Annotation
more info
 
sperm midpiece IEA
Inferred from Electronic Annotation
more info
 

General protein information

Preferred Names
sequestosome-1
Names
EBI3-associated protein of 60 kDa
EBI3-associated protein p60
EBIAP
autophagy receptor p62
oxidative stress induced like
phosphotyrosine independent ligand for the Lck SH2 domain p62
phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
ubiquitin-binding protein p62

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_011342.1 RefSeqGene

    Range
    5006..36691
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001142298.2NP_001135770.1  sequestosome-1 isoform 2

    See identical proteins and their annotated locations for NP_001135770.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation from an in-frame downstream start codon compared to variant 1. This results in an isoform (2) with a shorter N-terminus compared to isoform 1. Variants 2 and 3 encode the same isoform.
    Source sequence(s)
    BC000951, BC017222, BI549805, BU625974
    Consensus CDS
    CCDS47355.1
    UniProtKB/Swiss-Prot
    Q13501
    Conserved Domains (3) summary
    cd02340
    Location:4284
    ZZ_NBR1_like; Zinc finger, ZZ type. Zinc finger present in Drosophila ref(2)P, NBR1, Human sequestosome 1 and related proteins. The ZZ motif coordinates two zinc ions and most likely participates in ligand binding or molecular scaffolding. Drosophila ref(2)P appears ...
    cl02720
    Location:118
    PB1; The PB1 domain is a modular domain mediating specific protein-protein interactions which play a role in many critical cell processes, such as osteoclastogenesis, angiogenesis, early cardiovascular development, and cell polarity. A canonical PB1-PB1 ...
    pfam16577
    Location:295356
    UBA_5; UBA domain
  2. NM_001142299.2NP_001135771.1  sequestosome-1 isoform 2

    See identical proteins and their annotated locations for NP_001135771.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation from an in-frame downstream start codon compared to variant 1. This results in an isoform (2) with a shorter N-terminus compared to isoform 1. Variants 2 and 3 differ in the 5' UTR, but encode the same isoform.
    Source sequence(s)
    BC000951, BC017222, BU625974, DA541885
    Consensus CDS
    CCDS47355.1
    UniProtKB/Swiss-Prot
    Q13501
    Related
    ENSP00000353944.5, ENST00000360718.5
    Conserved Domains (3) summary
    cd02340
    Location:4284
    ZZ_NBR1_like; Zinc finger, ZZ type. Zinc finger present in Drosophila ref(2)P, NBR1, Human sequestosome 1 and related proteins. The ZZ motif coordinates two zinc ions and most likely participates in ligand binding or molecular scaffolding. Drosophila ref(2)P appears ...
    cl02720
    Location:118
    PB1; The PB1 domain is a modular domain mediating specific protein-protein interactions which play a role in many critical cell processes, such as osteoclastogenesis, angiogenesis, early cardiovascular development, and cell polarity. A canonical PB1-PB1 ...
    pfam16577
    Location:295356
    UBA_5; UBA domain
  3. NM_003900.5NP_003891.1  sequestosome-1 isoform 1

    See identical proteins and their annotated locations for NP_003891.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1).
    Source sequence(s)
    AI333228, BC000951, BC017222, DC398116
    Consensus CDS
    CCDS34317.1
    UniProtKB/Swiss-Prot
    Q13501
    Related
    ENSP00000374455.4, ENST00000389805.9
    Conserved Domains (3) summary
    cd06402
    Location:4102
    PB1_p62; The PB1 domain is an essential part of p62 scaffold protein (alias sequestosome 1,SQSTM) involved in cell signaling, receptor internalization, and protein turnover. The PB1 domain is a modular domain mediating specific protein-protein interaction which ...
    cd02340
    Location:126168
    ZZ_NBR1_like; Zinc finger, ZZ type. Zinc finger present in Drosophila ref(2)P, NBR1, Human sequestosome 1 and related proteins. The ZZ motif coordinates two zinc ions and most likely participates in ligand binding or molecular scaffolding. Drosophila ref(2)P appears ...
    pfam16577
    Location:379439
    UBA_5; UBA domain

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.20200815

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000005.10 Reference GRCh38.p13 Primary Assembly

    Range
    179806393..179838078
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Reference GRCh38.p13 PATCHES

Genomic

  1. NW_016107298.1 Reference GRCh38.p13 PATCHES

    Range
    571962..603638
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
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