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fleQ transcriptional regulator FleQ [ Pseudomonas aeruginosa PAO1 ]

Gene ID: 881960, updated on 30-Jul-2025
Gene symbol
fleQ
Gene description
transcriptional regulator FleQ
Locus tag
PA1097
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Pseudomonas aeruginosa PAO1 (strain: PAO1)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas
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Sequence:
NC_002516.2 (1187587..1189059)

NC_002516.2Genomic Context describing neighboring genes Neighboring gene B-type flagellar protein FliS Neighboring gene hypothetical protein Neighboring gene two-component sensor Neighboring gene two-component response regulator

Genomic Sequence:
NC_002516.2

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Sensor I Regulated ATPase Activity of FleQ Is Essential for Motility to Biofilm Transition in Pseudomonas aeruginosa. Banerjee P, et al. ACS Chem Biol, 2019 Jul 19. PMID 31268665
  2. The REC domain mediated dimerization is critical for FleQ from Pseudomonas aeruginosa to function as a c-di-GMP receptor and flagella gene regulator. Su T, et al. J Struct Biol, 2015 Oct. PMID 26362077
  3. A conservative amino acid mutation in the master regulator FleQ renders Pseudomonas aeruginosa aflagellate. Jain R, et al. PLoS One, 2014. PMID 24827992, Free PMC Article
  4. Cyclic diguanosine monophosphate represses bacterial flagella synthesis by interacting with the Walker A motif of the enhancer-binding protein FleQ. Baraquet C, et al. Proc Natl Acad Sci U S A, 2013 Nov 12. PMID 24167275, Free PMC Article
  5. FleQ, the major flagellar gene regulator in Pseudomonas aeruginosa, binds to enhancer sites located either upstream or atypically downstream of the RpoN binding site. Jyot J, et al. J Bacteriol, 2002 Oct. PMID 12218010, Free PMC Article

See all (21) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. This study determined the crystal structures of the ATPase domain of wild-type FleQ and sensor I mutants H287N and H287A in complex with ATPgammaS and Mg(2+) to 2.4, 1.95, and 2.25 A resolution, respectively.
    Title: Sensor I Regulated ATPase Activity of FleQ Is Essential for Motility to Biofilm Transition in Pseudomonas aeruginosa.
  2. FleQ mutation prevented flagellar assembly and reduced internalization of Pseudomonas aeruginosa independently of ExoS.
    Title: The Impact of ExoS on Pseudomonas aeruginosa Internalization by Epithelial Cells Is Independent of fleQ and Correlates with Bistability of Type Three Secretion System Gene Expression.
  3. The dimer is the functional form of FleN, and it is nucleotide binding and not hydrolysis by FleN that is necessary to exert an antagonistic effect against FleQ.
    Title: ATP-Induced Structural Remodeling in the Antiactivator FleN Enables Formation of the Functional Dimeric Form.
  4. The atypical REC domain of FleQ is essential for the function of FleQ.
    Title: The REC domain mediated dimerization is critical for FleQ from Pseudomonas aeruginosa to function as a c-di-GMP receptor and flagella gene regulator.
  5. The ability of FleQ to positively regulate flagellar gene expression.
    Title: A conservative amino acid mutation in the master regulator FleQ renders Pseudomonas aeruginosa aflagellate.
  6. Cyclic diguanosine monophosphate represses bacterial flagella synthesis by interacting with the Walker A motif of the enhancer-binding protein FleQ.
    Title: Cyclic diguanosine monophosphate represses bacterial flagella synthesis by interacting with the Walker A motif of the enhancer-binding protein FleQ.
  7. Analysis of wild type and mutated versions of pelA-lacZ transcriptional fusions suggests that FleQ represses gene expression from box 2 and activates gene expression in response to c-di-GMP from box 1.
    Title: The FleQ protein from Pseudomonas aeruginosa functions as both a repressor and an activator to control gene expression from the pel operon promoter in response to c-di-GMP.
  8. The results show that FleQ is a new type of cyclic diguanylate binding protein that controls the transcriptional regulation of EPS biosynthesis genes in P. aeruginosa.
    Title: Identification of FleQ from Pseudomonas aeruginosa as a c-di-GMP-responsive transcription factor.
Submit: New GeneRIF Correction

Homology

Gene Ontology Provided by PseudoCAP

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA-binding transcription activator activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables DNA-binding transcription activator activity IPI
Inferred from Physical Interaction
more info
 PubMed 
enables DNA-binding transcription repressor activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables DNA-binding transcription repressor activity IPI
Inferred from Physical Interaction
more info
 PubMed 
enables cis-regulatory region sequence-specific DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables cyclic-di-GMP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables cyclic-di-GMP binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables sequence-specific DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables transcription cis-regulatory region binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables transcription cis-regulatory region binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of extracellular matrix assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of cell-substrate adhesion IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of cilium-dependent cell motility IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of bacterial-type flagellum-dependent cell motility IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of protein-DNA complex IMP
Inferred from Mutant Phenotype
more info
 PubMed 
part_of protein-DNA complex IPI
Inferred from Physical Interaction
more info
 PubMed 
Preferred Names
transcriptional regulator FleQ
NP_249788.1
  • Product name confidence: class 1 (Function experimentally demonstrated in P. aeruginosa)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_002516.2 Reference assembly

    Range
    1187587..1189059
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_249788.1 transcriptional regulator FleQ [Pseudomonas aeruginosa PAO1]

    See identical proteins and their annotated locations for NP_249788.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    Q51452
    Conserved Domains (1) summary
    COG2204
    Location:1 → 480
    AtoC; DNA-binding transcriptional response regulator, NtrC family, contains REC, AAA-type ATPase, and a Fis-type DNA-binding domains [Signal transduction mechanisms]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
G3XCV0.1

Gene LinkOut

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Molecular Biology Databases
Research Materials