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vfr cAMP-regulatory protein [ Pseudomonas aeruginosa PAO1 ]

Gene ID: 880744, updated on 24-Aug-2024

Summary

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Gene symbol
vfr
Gene description
cAMP-regulatory protein
Locus tag
PA0652
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Pseudomonas aeruginosa PAO1 (strain: PAO1)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas
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Genomic context

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Sequence:
NC_002516.2 (706028..706672, complement)

NC_002516.2Genomic Context describing neighboring genes Neighboring gene anthranilate synthase component II Neighboring gene anthranilate phosphoribosyltransferase Neighboring gene indole-3-glycerol phosphate synthase Neighboring gene hypothetical protein Neighboring gene S-adenosylmethionine decarboxylase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_002516.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. cAMP and Vfr Control Exolysin Expression and Cytotoxicity of Pseudomonas aeruginosa Taxonomic Outliers. Berry A, et al. J Bacteriol, 2018 Jun 15. PMID 29632090, Free PMC Article
  2. Crystal structure of the Pseudomonas aeruginosa virulence factor regulator. Cordes TJ, et al. J Bacteriol, 2011 Aug. PMID 21665969, Free PMC Article
  3. Role of Vfr in regulating exotoxin A production by Pseudomonas aeruginosa. Davinic M, et al. Microbiology (Reading), 2009 Jul. PMID 19389782, Free PMC Article
  4. Emergence of secretion-defective sublines of Pseudomonas aeruginosa PAO1 resulting from spontaneous mutations in the vfr global regulatory gene. Fox A, et al. Appl Environ Microbiol, 2008 Mar. PMID 18203852, Free PMC Article
  5. Characterization of DNA-binding specificity and analysis of binding sites of the Pseudomonas aeruginosa global regulator, Vfr, a homologue of the Escherichia coli cAMP receptor protein. Kanack KJ, et al. Microbiology (Reading), 2006 Dec. PMID 17159200

See all (20) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Hfq and sRNA 179 Inhibit Expression of the Pseudomonas aeruginosa cAMP-Vfr and Type III Secretion Regulons.
    Title: Hfq and sRNA 179 Inhibit Expression of the Pseudomonas aeruginosa cAMP-Vfr and Type III Secretion Regulons.
  2. The exlB and exlA expression was diminished in the Vfr-negative mutant and upregulated with increased intracellular cAMP levels.
    Title: cAMP and Vfr Control Exolysin Expression and Cytotoxicity of Pseudomonas aeruginosa Taxonomic Outliers.
  3. study reports that Vfr directly controls exsA transcription, the master regulator of T3SS gene expression, from a newly identified promoter located immediately upstream of exsA
    Title: Vfr Directly Activates exsA Transcription To Regulate Expression of the Pseudomonas aeruginosa Type III Secretion System.
  4. findings led to the discovery of an interplay between FimV and the global regulator Vfr triggering the modulation of the level of Vfr and consequently the modulation of T2SS-dependent secretion on solid medium.
    Title: Role of fimV in type II secretion system-dependent protein secretion of Pseudomonas aeruginosa on solid medium.
  5. Vfr directly regulates rhlR transcription through its binding to several Vfr-binding sites (VBSs) present in the rhlR promoter region.
    Title: Transcriptional regulation of Pseudomonas aeruginosa rhlR: role of the CRP orthologue Vfr (virulence factor regulator) and quorum-sensing regulators LasR and RhlR.
  6. The crystal structure of Vfr shows that it is a winged-helix DNA-binding protein like its homologue cyclic AMP receptor protein (CRP).
    Title: Crystal structure of the Pseudomonas aeruginosa virulence factor regulator.
  7. Vfr enhances toxA expression in PAO1 both directly and indirectly through regA, but not through pvdS;vfr expression is not regulated by iron;both Vfr and PvdS cooperate in the presence of RegA to achieve a maximum level of toxA expression.
    Title: Role of Vfr in regulating exotoxin A production by Pseudomonas aeruginosa.
  8. In a reconstruction experiment conducted with a 1:1 mixture of wild-type and a vfr mutant of PAO1, data showed that the vfr mutant had a selective advantage over the wild type after growth in static culture for 4 days.
    Title: Emergence of secretion-defective sublines of Pseudomonas aeruginosa PAO1 resulting from spontaneous mutations in the vfr global regulatory gene.
  9. The results suggest that Vfr modulates toxA and lasB expression in PAO1 through PtxR; a model defining the relationships between these different genes is presented.
    Title: Regulation of Pseudomonas aeruginosa ptxR by Vfr.
  10. study suggests that Vfr shares many of the same functions as cAMP receptor protein, CRP, but has specialized functions, at least in terms of DNA target sequence binding, required for regulation of a subset of genes in its regulon
    Title: Characterization of DNA-binding specificity and analysis of binding sites of the Pseudomonas aeruginosa global regulator, Vfr, a homologue of the Escherichia coli cAMP receptor protein.
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General gene information

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Homology

General protein information

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Preferred Names
cAMP-regulatory protein
NP_249343.1
  • Product name confidence: class 1 (Function experimentally demonstrated in P. aeruginosa)

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_002516.2 Reference assembly

    Range
    706028..706672 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_249343.1 cAMP-regulatory protein [Pseudomonas aeruginosa PAO1]

    See identical proteins and their annotated locations for NP_249343.1

    Status: PROVISIONAL

    UniProtKB/Swiss-Prot
    P55222
    Conserved Domains (1) summary
    PRK11753
    Location:1 → 214
    PRK11753; cAMP-activated global transcriptional regulator CRP
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P55222.1

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