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SPT15 TATA-binding protein [ Saccharomyces cerevisiae S288C ]

Gene ID: 856891, updated on 2-Nov-2024

Summary

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Official Symbol
SPT15
Official Full Name
TATA-binding protein
Primary source
SGD:S000000950
Locus tag
YER148W
See related
AllianceGenome:SGD:S000000950; FungiDB:YER148W; VEuPathDB:YER148W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
BTF1; TBP1
Summary
Enables several functions, including DNA binding activity; RNA polymerase I general transcription initiation factor binding activity; and RNA polymerase II-specific DNA-binding transcription factor binding activity. Involved in DNA-templated transcription. Located in nucleus. Part of transcription factor TFIID complex and transcription factor TFIIIB complex. Used to study spinocerebellar ataxia type 17. Human ortholog(s) of this gene implicated in late onset Parkinson's disease; schizophrenia; spinocerebellar ataxia type 17; and type 1 diabetes mellitus. Orthologous to several human genes including TBPL2 (TATA-box binding protein like 2). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See SPT15 in Genome Data Viewer
Location:
chromosome: V
Exon count:
1
Sequence:
Chromosome: V; NC_001137.3 (465303..466025)

Chromosome V - NC_001137.3Genomic Context describing neighboring genes Neighboring gene uncharacterized protein Neighboring gene RNA-binding protein LSM5 Neighboring gene cohesin-loading factor complex subunit SCC4 Neighboring gene Pea2p Neighboring gene Spi1p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The TATA-binding protein from Saccharomyces cerevisiae oligomerizes in solution at micromolar concentrations to form tetramers and octamers. Daugherty MA, et al. J Mol Biol, 1999 Jan 29. PMID 9917384
  2. Use of a combined enzymatic digestion/ESI mass spectrometry assay to study the effect of TATA-binding protein on photoproduct formation in a TATA box. Wang Y, et al. Biochemistry, 2001 Oct 2. PMID 11570879
  3. Kinetic preference for oriented DNA binding by the yeast TATA-binding protein TBP. Liu Y, et al. Biochemistry, 2001 May 29. PMID 11371187
  4. Multiple functions of the nonconserved N-terminal domain of yeast TATA-binding protein. Lee M, et al. Genetics, 2001 May. PMID 11333220, Free PMC Article
  5. Reevaluation of transcriptional regulation by TATA-binding protein oligomerization: predominance of monomers. Campbell KM, et al. Biochemistry, 2000 Mar 14. PMID 10704213

See all (469) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Structural convergence endows nuclear transport receptor Kap114p with a transcriptional repressor function toward TATA-binding protein.
    Title: Structural convergence endows nuclear transport receptor Kap114p with a transcriptional repressor function toward TATA-binding protein.
  2. Connection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters.
    Title: Connection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters.
  3. Architecture of the multi-functional SAGA complex and the molecular mechanism of holding TBP.
    Title: Architecture of the multi-functional SAGA complex and the molecular mechanism of holding TBP.
  4. structure of yeast SAGA with bound TBP
    Title: Structure of SAGA and mechanism of TBP deposition on gene promoters.
  5. Transcription of nearly all yeast RNA polymerase II-transcribed genes is dependent on transcription factor TFIID.
    Title: SAGA Is a General Cofactor for RNA Polymerase II Transcription.
  6. Transcription of nearly all yeast RNA polymerase II-transcribed genes is dependent on transcription factor TFIID.
    Title: Transcription of Nearly All Yeast RNA Polymerase II-Transcribed Genes Is Dependent on Transcription Factor TFIID.
  7. this study identified two SPT15 alleles, nine gene deletions and low concentration of amino acids in the medium that confer enhanced tolerance to acetic acid.
    Title: Tolerance to acetic acid is improved by mutations of the TATA-binding protein gene.
  8. SPT15 confers enhanced tolerance to hyperosmotic stress.
    Title: Mutations of the TATA-binding protein confer enhanced tolerance to hyperosmotic stress in Saccharomyces cerevisiae.
  9. The action of Mot1 is required to clear TBP from intrinsically preferred (TATA-containing) binding sites, ensuring sufficient soluble TBP to bind intrinsically disfavored (TATA-less) sites.
    Title: Mot1 redistributes TBP from TATA-containing to TATA-less promoters.
  10. In the absence of ATP, ternary complex stability arises from both Mot1-DNA and Mot1-TBP interactions.
    Title: Two-step mechanism for modifier of transcription 1 (Mot1) enzyme-catalyzed displacement of TATA-binding protein (TBP) from DNA.
Submit: New GeneRIF Correction See all GeneRIFs (23)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA binding, bending IDA
Inferred from Direct Assay
more info
 PubMed 
enables RNA polymerase I general transcription initiation factor binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables RNA polymerase I general transcription initiation factor binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables RNA polymerase II core promoter sequence-specific DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables RNA polymerase II general transcription initiation factor activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables RNA polymerase II-specific DNA-binding transcription factor binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables RNA polymerase II-specific DNA-binding transcription factor binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables RNA polymerase III transcription regulatory region sequence-specific DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables chromatin binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA-templated transcription initiation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in DNA-templated transcription initiation IEA
Inferred from Electronic Annotation
more info
  
involved_in RNA polymerase I preinitiation complex assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in RNA polymerase II preinitiation complex assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in RNA polymerase III preinitiation complex assembly IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in nucleolar large rRNA transcription by RNA polymerase I IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
part_of transcription factor TFIID complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of transcription factor TFIIIB complex IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
TATA-binding protein
NP_011075.3
  • TATA-binding protein (TBP); general transcription factor that interacts with other factors to form the preinitiation complex at promoters; essential for viability, highly conserved; yeast gene can complement mutations in human homolog TBP

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001137.3 Reference assembly

    Range
    465303..466025
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001179038.3 → NP_011075.3  TPA: TATA-binding protein [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_011075.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D3DM55, P13393
    UniProtKB/TrEMBL
    A6ZRA5, B3LRR1, B5VHS6, C7GQ48, C8Z7H0, G2WD35, G4XSE0, G4XSG8, G4XSJ4, G4XSK3, N1P3M9
    Conserved Domains (1) summary
    PLN00062
    Location:64 → 239
    PLN00062; TATA-box-binding protein; Provisional
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P13393.3

Gene LinkOut

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