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VAC8 protein anchor VAC8 [ Saccharomyces cerevisiae S288C ]

Gene ID: 856702, updated on 12-Aug-2025
Official Symbol
VAC8
Official Full Name
protein anchor VAC8
Primary source
SGD:S000000739
Locus tag
YEL013W
See related
AllianceGenome:SGD:S000000739; FungiDB:YEL013W; VEuPathDB:YEL013W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
YEB3
Summary
Enables protein-membrane adaptor activity. Involved in several processes, including Cvt vesicle assembly; macroautophagy; and vacuole organization. Acts upstream of or within establishment of organelle localization; protein localization to phagophore assembly site; and protein-containing complex localization. Located in several cellular components, including fungal-type vacuole membrane; nuclear membrane; and nucleus-vacuole junction. Part of Myo2p-Vac17p-Vac8p transport complex. Orthologous to human ARMC3 (armadillo repeat containing 3). [provided by Alliance of Genome Resources, Apr 2025]
Orthologs
all
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See VAC8 in Genome Data Viewer
Location:
chromosome: V
Exon count:
1
Sequence:
Chromosome: V; NC_001137.3 (128825..130561)

Chromosome V - NC_001137.3Genomic Context describing neighboring genes Neighboring gene Edc3p Neighboring gene uncharacterized protein Neighboring gene tRNA-Gln Neighboring gene E2 ubiquitin-conjugating protein UBC8

Genomic Sequence:
NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Multiple classes of yeast mutants are defective in vacuole partitioning yet target vacuole proteins correctly. Wang YX, et al. Mol Biol Cell, 1996 Sep. PMID 8885233, Free PMC Article
  2. Palmitoylation plays a role in targeting Vac8p to specific membrane subdomains. Peng Y, et al. Traffic, 2006 Oct. PMID 16978392
  3. YEB3/VAC8 encodes a myristylated armadillo protein of the Saccharomyces cerevisiae vacuolar membrane that functions in vacuole fusion and inheritance. Pan X, et al. J Cell Sci, 1998 Aug. PMID 9664035
  4. Yel013p (Vac8p), an armadillo repeat protein related to plakoglobin and importin alpha is associated with the yeast vacuole membrane. Fleckenstein D, et al. J Cell Sci, 1998 Oct. PMID 9739084
  5. A novel cold-sensitive allele of the rate-limiting enzyme of fatty acid synthesis, acetyl coenzyme A carboxylase, affects the morphology of the yeast vacuole through acylation of Vac8p. Schneiter R, et al. Mol Cell Biol, 2000 May. PMID 10757783, Free PMC Article

See all (144) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The Atg1 complex, Atg9, and Vac8 recruit PI3K complex I to the pre-autophagosomal structure.
    Title: The Atg1 complex, Atg9, and Vac8 recruit PI3K complex I to the pre-autophagosomal structure.
  2. Structures of Vac8-containing protein complexes reveal the underlying mechanism by which Vac8 regulates multiple cellular processes.
    Title: Structures of Vac8-containing protein complexes reveal the underlying mechanism by which Vac8 regulates multiple cellular processes.
  3. Vac8 determines phagophore assembly site vacuolar localization during nitrogen starvation-induced autophagy.
    Title: Vac8 determines phagophore assembly site vacuolar localization during nitrogen starvation-induced autophagy.
  4. Quaternary structures of Vac8 differentially regulate the Cvt and PMN pathways.
    Title: Quaternary structures of Vac8 differentially regulate the Cvt and PMN pathways.
  5. Authors show that Vac8 constitutes a vacuolar tether that stably anchors the PAS to the vacuole throughout autophagosome biogenesis via the PAS component Atg13. S. cerevisiae lacking Vac8 show inefficient autophagosome-vacuole fusion, and form fewer and smaller autophagosomes that often localize away from the vacuole.
    Title: Vac8 spatially confines autophagosome formation at the vacuole in S. cerevisiae.
  6. Study found that non-selective bulk autophagy of cytosolic proteins as well as the selective autophagic degradation of peroxisomes (pexophagy) and ribosomes (ribophagy) was dependent on the armadillo repeat protein Vac8 in Saccharomyces cerevisiae. Moreover, pexophagy and ribophagy depended on the palmitoylation of Vac8.
    Title: Vac8 Controls Vacuolar Membrane Dynamics during Different Autophagy Pathways in Saccharomyces cerevisiae.
  7. Report the crystal structure of Vac8p bound to Nvj1p at 2.4-A resolution. Vac8p comprises a flexibly connected N-terminal H1 helix followed by 12 armadillo repeats (ARMs) that form a right-handed superhelical structure. The extended 80-A-long loop of Nvj1p specifically binds the highly conserved inner groove formed from ARM1-12 of Vac8p.
    Title: Mechanistic insight into the nucleus-vacuole junction based on the Vac8p-Nvj1p crystal structure.
  8. Data suggest that a major role of Vac8p is to spatially separate multiple functions thereby enabling vacuole inheritance to occur concurrently with other vacuolar processes.
    Title: Vac8p, an armadillo repeat protein, coordinates vacuole inheritance with multiple vacuolar processes.
  9. Here, we address the role of Vac8 palmitoylation and show that the position and number of substrate cysteines within the SH4 domain determine the vacuole localization of Vac8: stable vacuole binding of Vac8 requires two cysteines within the N-terminus.
    Title: Palmitoylation determines the function of Vac8 at the yeast vacuole.
  10. Data show that each of the three Vac8p putative palmitoylation sites, at Cys 4, 5 and 7, may serve as a palmitoylation site.
    Title: Palmitoylation plays a role in targeting Vac8p to specific membrane subdomains.
Submit: New GeneRIF Correction See all GeneRIFs (11)
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein-membrane adaptor activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein-membrane adaptor activity IEA
Inferred from Electronic Annotation
more info
  
enables protein-membrane adaptor activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables protein-membrane adaptor activity IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in Cvt vesicle assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in autophagosome assembly IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in autophagosome assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in autophagy IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within establishment of organelle localization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in lipid metabolic process NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in macroautophagy IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in nucleus-vacuole junction assembly IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in nucleus-vacuole junction assembly IEA
Inferred from Electronic Annotation
more info
  
involved_in nucleus-vacuole junction assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in pexophagy IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in piecemeal microautophagy of the nucleus IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in piecemeal microautophagy of the nucleus NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in protein localization to membrane raft IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within protein localization to phagophore assembly site IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein localization to phagophore assembly site IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within protein-containing complex localization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in ribophagy IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in vacuole fusion, non-autophagic IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in vacuole inheritance IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in vacuole inheritance IEA
Inferred from Electronic Annotation
more info
  
involved_in vacuole inheritance IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of Myo2p-Vac17p-Vac8p transport complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of Myo2p-Vac17p-Vac8p transport complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in cytosol HDA PubMed 
located_in fungal-type vacuole membrane HDA PubMed 
is_active_in fungal-type vacuole membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in fungal-type vacuole membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in membrane raft IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nuclear membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nuclear outer membrane NAS
Non-traceable Author Statement
more info
 PubMed 
located_in nucleus-vacuole junction IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus-vacuole junction IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in phagophore assembly site IMP
Inferred from Mutant Phenotype
more info
 PubMed 
located_in vacuolar membrane IEA
Inferred from Electronic Annotation
more info
  
located_in vacuole IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
protein anchor VAC8
NP_010903.3
  • Vacuole-specific Myo2p receptor; Myo2p-Vac17p-Vac8p transport complex subunit required for vacuolar inheritance; mediates anchoring of phosphatidylinositol 3-kinase complex I (PIK3C3-C1) to autophagosome; required with Atg13p for the vesicle closure step of the cytoplasm-to-vacuole (CVT) pathway, for homotypic vacuole-vacuole fusion and for nucleus-vacuole junction formation with Nvj1p; contains 11 armadillo (ARM) repeats; myristoylated, palmitoylated, and phosphorylated

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001137.3 Reference assembly

    Range
    128825..130561
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001178828.3 → NP_010903.3  TPA: protein anchor VAC8

    See identical proteins and their annotated locations for NP_010903.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D3DLN6, P39968, Q549T0
    UniProtKB/TrEMBL
    A0A0L8VS39, A6ZQT6, B3LS03, C8Z6Y7, G2WCL9, N1P5M6
    Conserved Domains (2) summary
    COG5064
    Location:42 → 358
    SRP1; Karyopherin (importin) alpha [Intracellular trafficking and secretion]
    sd00043
    Location:204 → 235
    ARM; armadillo repeat [structural motif]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P39968.3

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