SPF1 ion-transporting P-type ATPase SPF1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SPF1
Official Full Name: ion-transporting P-type ATPase SPF1
Primary source: SGD:S000000757
Locus tag: YEL031W
See related: AllianceGenome:SGD:S000000757; FungiDB:YEL031W; VEuPathDB:YEL031W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: COD1; PER9; PIO1
Summary: Enables phosphatidylinositol-4-phosphate binding activity. Involved in intracellular calcium ion homeostasis; intracellular manganese ion homeostasis; and sterol homeostasis. Located in cis-Golgi network and endoplasmic reticulum membrane. Orthologous to human ATP13A1 (ATPase 13A1). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs: all
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Genomic context

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See SPF1 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (90258..93905)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The effect of metal ions on the Spf1p P5A-ATPase. High sensitivity to irreversible inhibition by zinc.
Title: The effect of metal ions on the Spf1p P5A-ATPase. High sensitivity to irreversible inhibition by zinc.
The Spf1p P5A-ATPase ""arm-like"" domain is not essential for ATP hydrolysis but its deletion impairs autophosphorylation.
Title: The Spf1p P5A-ATPase "arm-like" domain is not essential for ATP hydrolysis but its deletion impairs autophosphorylation.
Highly exposed segment of the Spf1p P5A-ATPase near transmembrane M5 detected by limited proteolysis.
Title: Highly exposed segment of the Spf1p P5A-ATPase near transmembrane M5 detected by limited proteolysis.
The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.
Title: The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.
Inhibition of the Formation of the Spf1p Phosphoenzyme by Ca2.
Title: Inhibition of the Formation of the Spf1p Phosphoenzyme by Ca2.
Studies indicate a role of the endogenous P5A-ATPase Spf1p in separation of charges in endoplasmic reticulum (ER) and the sterol metabolism.
Title: Towards defining the substrate of orphan P5A-ATPases.
role of spf1 in manganese transport into the endoplasmic reticulum
Title: The yeast p5 type ATPase, spf1, regulates manganese transport into the endoplasmic reticulum.
Shadows of an absent partner: ATP hydrolysis and phosphoenzyme turnover of the Spf1 (sensitivity to Pichia farinosa killer toxin) P5-ATPase.
Title: Shadows of an absent partner: ATP hydrolysis and phosphoenzyme turnover of the Spf1 (sensitivity to Pichia farinosa killer toxin) P5-ATPase.
In mutants that lack Spf1p, a mutation in the gene Mdm39p is lethal.
Title: Cooperative function of the CHD5-like protein Mdm39p with a P-type ATPase Spf1p in the maintenance of ER homeostasis in Saccharomyces cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables P-type transmembrane transporter activity	IEA Inferred from Electronic Annotation more info
enables membrane protein dislocase activity	IBA Inferred from Biological aspect of Ancestor more info
enables membrane protein dislocase activity	IDA Inferred from Direct Assay more info	PubMed
enables membrane protein dislocase activity	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol-4-phosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in extraction of mislocalized protein from ER membrane	IDA Inferred from Direct Assay more info	PubMed
involved_in intracellular calcium ion homeostasis	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular calcium ion homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular manganese ion homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein hexamerization	IDA Inferred from Direct Assay more info	PubMed
involved_in protein unfolding	IDA Inferred from Direct Assay more info	PubMed
involved_in sterol homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in cis-Golgi network	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endoplasmic reticulum membrane	EXP Inferred from Experiment more info	PubMed
is_active_in endoplasmic reticulum membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed

General protein information

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Preferred Names: ion-transporting P-type ATPase SPF1

NP_010883.3

P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid and sterol composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1; null mutation is complemented by human Parkinson disease-related ATP13A1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001137.3 Reference assembly

Range

90258..93905

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178846.3 → NP_010883.3 ion-transporting P-type ATPase SPF1

See identical proteins and their annotated locations for NP_010883.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DLL8, P39986

UniProtKB/TrEMBL

B3LRY5

Conserved Domains (1) summary

cd07543
Location:169 → 1136

P-type_ATPase_cation; P-type cation-transporting ATPases, similar to human cation-transporting ATPase type 13A1 (ATP13A1) and Saccharomyces manganese-transporting ATPase 1 Spf1p