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TRM5 tRNA (guanine) methyltransferase [ Saccharomyces cerevisiae S288C ]

Gene ID: 856467, updated on 23-Apr-2026
Official Symbol
TRM5
Official Full Name
tRNA (guanine) methyltransferase
Primary source
SGD:S000001112
Locus tag
YHR070W
See related
AllianceGenome:SGD:S000001112; FungiDB:YHR070W; VEuPathDB:YHR070W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables tRNA (guanine(37)-N1)-methyltransferase activity. Involved in mitochondrial tRNA methylation and tRNA N1-guanine methylation. Located in mitochondrial matrix. Used to study several diseases, including hypercalcemia; hypertrophic cardiomyopathy; lactic acidosis; mitochondrial complex III deficiency; and mitochondrial myopathy. Human ortholog(s) of this gene implicated in combined oxidative phosphorylation deficiency 26. Orthologous to human TRMT5 (tRNA methyltransferase 5). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs
all
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See TRM5 in Genome Data Viewer
Location:
chromosome: VIII
Exon count:
1
Sequence:
Chromosome: VIII; NC_001140.6 (234881..236380)

Chromosome VIII - NC_001140.6Genomic Context describing neighboring genes Neighboring gene deoxyhypusine synthase Neighboring gene exosome non-catalytic core subunit RRP4 Neighboring gene Pcl5p Neighboring gene tRNA-Phe

Genomic Sequence:
NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. A primordial tRNA modification required for the evolution of life? Björk GR, et al. EMBO J, 2001 Jan 15. PMID 11226173, Free PMC Article
  2. Yeast mitochondrial initiator tRNA is methylated at guanosine 37 by the Trm5-encoded tRNA (guanine-N1-)-methyltransferase. Lee C, et al. J Biol Chem, 2007 Sep 21. PMID 17652090
  3. Retrograde nuclear import of tRNA precursors is required for modified base biogenesis in yeast. Ohira T, et al. Proc Natl Acad Sci U S A, 2011 Jun 28. PMID 21670254, Free PMC Article
  4. TRMT5 Mutations Cause a Defect in Post-transcriptional Modification of Mitochondrial tRNA Associated with Multiple Respiratory-Chain Deficiencies. Powell CA, et al. Am J Hum Genet, 2015 Aug 6. PMID 26189817, Free PMC Article
  5. Detection and discovery of RNA modifications using microarrays. Hiley SL, et al. Nucleic Acids Res, 2005 Jan 7. PMID 15640439, Free PMC Article

See all (25) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. mitochondrial initiator tRNA is methylated at guanosine 37 by the Trm5-encoded tRNA (guanine-N1-)-methyltransferase
    Title: Yeast mitochondrial initiator tRNA is methylated at guanosine 37 by the Trm5-encoded tRNA (guanine-N1-)-methyltransferase.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables tRNA (guanine(37)-N1)-methyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables tRNA (guanine(37)-N1)-methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables tRNA (guanine(37)-N1)-methyltransferase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables tRNA (guanine(37)-N1)-methyltransferase activity TAS
Traceable Author Statement
more info
  
enables tRNA methyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
Process Evidence Code Pubs
involved_in mitochondrial tRNA methylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in mitochondrial tRNA methylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in mitochondrial tRNA methylation IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA N1-guanine methylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in tRNA N1-guanine methylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in tRNA N1-guanine methylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in tRNA methylation IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA modification TAS
Traceable Author Statement
more info
  
Component Evidence Code Pubs
located_in cytoplasm HDA PubMed 
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in mitochondrial matrix IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in mitochondrial matrix IDA
Inferred from Direct Assay
more info
 PubMed 
located_in mitochondrial matrix IEA
Inferred from Electronic Annotation
more info
  
located_in nucleoplasm TAS
Traceable Author Statement
more info
  
located_in nucleus HDA PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
tRNA (guanine) methyltransferase
NP_011937.1
  • tRNA(m(1)G37)methyltransferase; methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; localized to both cytoplasm and mitochondria, and modifies both cytoplasmic and mitochondrial tRNAs; mutations in human ortholog TRMT5 are associated with skeletal muscle respiratory chain deficiencies, and trm5 mutations analogous to disease mutations decrease respiration

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001140.6 Reference assembly

    Range
    234881..236380
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001179200.1 → NP_011937.1  tRNA (guanine) methyltransferase

    See identical proteins and their annotated locations for NP_011937.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D3DL19, P38793
    UniProtKB/TrEMBL
    A6ZSX6, B3LSI8, C7GWT1, C8Z9K9, G2WFE2
    Conserved Domains (1) summary
    COG2520
    Location:75 → 459
    Trm5; tRNA G37 N-methylase Trm5 [Translation, ribosomal structure and biogenesis]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P38793.1

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