MCM4 MCM DNA helicase complex subunit MCM4 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: MCM4
Gene description: MCM DNA helicase complex subunit MCM4
Primary source: SGD:S000006223
Locus tag: YPR019W
Gene type: protein coding
RNA name: MCM DNA helicase complex subunit MCM4
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CDC54; HCD21

Genomic context

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See MCM4 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (596750..599551)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

Sld3 binds specifically to DDK-phosphorylated peptides from two MCM subunits (Mcm4, 6) and then recruits Cdc45.
Title: Phosphopeptide binding by Sld3 links Dbf4-dependent kinase to MCM replicative helicase activation.
The Dbf4-dependent Cdc7 kinase (DDK), cyclin-dependent kinase (CDK), and Mec1 all target the structurally disordered N-terminal serine/threonine-rich domain of Mcm4, a subunit of the mini-chromosome maintenance replicative helicase complex.
Title: Domain within the helicase subunit Mcm4 integrates multiple kinase signals to control DNA replication initiation and fork progression.
constitutive overexpression of each individual MCM subunit was examined, and genotoxic sensitivity was found to be specific to Mcm2 or Mcm4 overexpression, further pointing to the importance of the DDK-MCM ring interaction.
Title: Dbf4 and Cdc7 proteins promote DNA replication through interactions with distinct Mcm2-7 protein subunits.
A defective MCM helicase causes genetic instability only in particular cell types.
Title: Ploidy dictates repair pathway choice under DNA replication stress.
The minimal functional unit of Mcm4/6/7 is a hexamer
Title: The effects of oligomerization on Saccharomyces cerevisiae Mcm4/6/7 function.
studies establish that the eukaryote-specific NSD of Mcm4 has evolved to integrate several protein kinase regulatory signals for progression through S phase
Title: The Dbf4-Cdc7 kinase promotes S phase by alleviating an inhibitory activity in Mcm4.
DNA runs through a central channel in the double hexamer, and, once loaded, Mcm2-7 can slide passively along double-stranded DNA.
Title: Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing.
using Saccharomyces cerevisiae containing a mcm4 helicase allele that was shown to cause cancer in mice.
Title: Aneuploidy and improved growth are coincident but not causal in a yeast cancer model.
The Mcm4/Mcm7 and Mcm4/Mcm6/Mcm7 assemblies can open to load onto circular DNA to initiate unwinding.
Title: Mcm subunits can assemble into two different active unwinding complexes.
Budding yeast Mcm4 is phosphorylated in vivo during S phase in a manner dependent on the presence of five CDK phosphoacceptor residues within the N-terminal domain of Mcm4.
Title: Interplay between S-cyclin-dependent kinase and Dbf4-dependent kinase in controlling DNA replication through phosphorylation of yeast Mcm4 N-terminal domain.

Submit: New GeneRIF Correction

Pathways from BioSystems

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Activation of the pre-replicative complex, organism-specific biosystem (from REACTOME)
Activation of the pre-replicative complex, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Cell Cycle, organism-specific biosystem (from REACTOME)
Cell Cycle, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Cell Cycle and Cell Division, organism-specific biosystem (from WikiPathways)
Cell Cycle and Cell Division, organism-specific biosystem(From http://en.wikipedia.org/wiki/Cell_cycle) The cell cycle is the series of events that takes place in a cell leading to its division and duplication (replication). Regulation of the cell cycle in...
Cell Cycle, Mitotic, organism-specific biosystem (from REACTOME)
Cell Cycle, Mitotic, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Cell cycle - yeast, organism-specific biosystem (from KEGG)
Cell cycle - yeast, organism-specific biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps (G1 and G2 phases). G1, S, and G...
Cell cycle - yeast, conserved biosystem (from KEGG)
Cell cycle - yeast, conserved biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps (G1 and G2 phases). G1, S, and G...
DNA Replication, organism-specific biosystem (from WikiPathways)
DNA Replication, organism-specific biosystemDNA replication, the basis for biological inheritance, is a fundamental process occurring in all living organisms to copy their DNA. This process is "replication" in that each strand of the original ...
DNA Replication, organism-specific biosystem (from REACTOME)
DNA Replication, organism-specific biosystemcomputationally inferred pathway (not manually curated)
DNA Replication Pre-Initiation, organism-specific biosystem (from REACTOME)
DNA Replication Pre-Initiation, organism-specific biosystemcomputationally inferred pathway (not manually curated)
DNA replication, organism-specific biosystem (from KEGG)
DNA replication, organism-specific biosystemA complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (D...
DNA replication, conserved biosystem (from KEGG)
DNA replication, conserved biosystemA complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (D...
DNA strand elongation, organism-specific biosystem (from REACTOME)
DNA strand elongation, organism-specific biosystemcomputationally inferred pathway (not manually curated)
G1/S Transition, organism-specific biosystem (from REACTOME)
G1/S Transition, organism-specific biosystemcomputationally inferred pathway (not manually curated)
M/G1 Transition, organism-specific biosystem (from REACTOME)
M/G1 Transition, organism-specific biosystemcomputationally inferred pathway (not manually curated)
MCM complex, organism-specific biosystem (from KEGG)
MCM complex, organism-specific biosystemStructural complex; Genetic information processing; Replication system
MCM complex, conserved biosystem (from KEGG)
MCM complex, conserved biosystemStructural complex; Genetic information processing; Replication system
Meiosis - yeast, organism-specific biosystem (from KEGG)
Meiosis - yeast, organism-specific biosystemDuring meiosis, a single round of DNA replication is followed by two rounds of chromosome segregation, called meiosis I and meiosis II. At meiosis I, one pair of sister chromatids recombines with, th...
Meiosis - yeast, conserved biosystem (from KEGG)
Meiosis - yeast, conserved biosystemDuring meiosis, a single round of DNA replication is followed by two rounds of chromosome segregation, called meiosis I and meiosis II. At meiosis I, one pair of sister chromatids recombines with, th...
Mitotic G1-G1/S phases, organism-specific biosystem (from REACTOME)
Mitotic G1-G1/S phases, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Orc1 removal from chromatin, organism-specific biosystem (from REACTOME)
Orc1 removal from chromatin, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Regulation of DNA replication, organism-specific biosystem (from REACTOME)
Regulation of DNA replication, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Removal of licensing factors from origins, organism-specific biosystem (from REACTOME)
Removal of licensing factors from origins, organism-specific biosystemcomputationally inferred pathway (not manually curated)
S Phase, organism-specific biosystem (from REACTOME)
S Phase, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Switching of origins to a post-replicative state, organism-specific biosystem (from REACTOME)
Switching of origins to a post-replicative state, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Synthesis of DNA, organism-specific biosystem (from REACTOME)
Synthesis of DNA, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Unwinding of DNA, organism-specific biosystem (from REACTOME)
Unwinding of DNA, organism-specific biosystemcomputationally inferred pathway (not manually curated)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Homologs of the MCM4 gene: The MCM4 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.cerevisiae, K.lactis, E.gossypii, S.pombe, M.oryzae, N.crassa, A.thaliana, and frog.
The Hierarchical Catalog of Orthologs

Gene Ontology Provided by GO

Function	Evidence Code	Pubs
ATP binding	IEA Inferred from Electronic Annotation more info
contributes_to ATP-dependent 3'-5' DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to ATP-dependent DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to ATP-dependent four-way junction helicase activity	IDA Inferred from Direct Assay more info	PubMed
DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
DNA helicase activity	IEA Inferred from Electronic Annotation more info
DNA replication origin binding	IDA Inferred from Direct Assay more info	PubMed
helicase activity	IEA Inferred from Electronic Annotation more info
hydrolase activity	IEA Inferred from Electronic Annotation more info
nucleotide binding	IEA Inferred from Electronic Annotation more info
single-stranded DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
contributes_to single-stranded DNA-dependent ATP-dependent DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to single-stranded DNA-dependent ATPase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
DNA replication	IEA Inferred from Electronic Annotation more info
DNA replication initiation	IEA Inferred from Electronic Annotation more info
DNA replication initiation	IGI Inferred from Genetic Interaction more info	PubMed
DNA strand elongation involved in DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
DNA unwinding involved in DNA replication	IDA Inferred from Direct Assay more info	PubMed
cell cycle	IEA Inferred from Electronic Annotation more info
double-strand break repair via break-induced replication	IMP Inferred from Mutant Phenotype more info	PubMed
nuclear DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
pre-replicative complex assembly involved in nuclear cell cycle DNA replication	IDA Inferred from Direct Assay more info	PubMed
pre-replicative complex assembly involved in nuclear cell cycle DNA replication	IPI Inferred from Physical Interaction more info	PubMed

Component	Evidence Code	Pubs
CMG complex	IDA Inferred from Direct Assay more info	PubMed
DNA replication preinitiation complex	IDA Inferred from Direct Assay more info	PubMed
MCM complex	IDA Inferred from Direct Assay more info	PubMed
MCM complex	IEA Inferred from Electronic Annotation more info
MCM core complex	IDA Inferred from Direct Assay more info	PubMed
cytoplasm	IDA Inferred from Direct Assay more info	PubMed
nuclear pre-replicative complex	IDA Inferred from Direct Assay more info	PubMed
nuclear replication fork	IDA Inferred from Direct Assay more info	PubMed
nucleus	IDA Inferred from Direct Assay more info	PubMed
nucleus	IEA Inferred from Electronic Annotation more info
replication fork protection complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: MCM DNA helicase complex subunit MCM4

NP_015344.1

EC 3.6.4.12

Essential helicase component of heterohexameric MCM2-7 complexes; MCM2-7 complexes bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

596750..599551

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184116.1 → NP_015344.1 TPA: MCM DNA helicase complex subunit MCM4 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015344.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P30665

Conserved Domains (2) summary

smart00350
Location:310 → 834

MCM; minichromosome maintenance proteins

pfam14551
Location:194 → 307

MCM_N; MCM N-terminal domain