RPA135 DNA-directed RNA polymerase I core subunit RPA135 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RPA135
Official Full Name: DNA-directed RNA polymerase I core subunit RPA135
Primary source: SGD:S000006214
Locus tag: YPR010C
See related: AllianceGenome:SGD:S000006214; FungiDB:YPR010C; VEuPathDB:YPR010C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: RPA2; RRN2; SRP3
Summary: Contributes to DNA-directed RNA polymerase activity. Involved in nucleolar large rRNA transcription by RNA polymerase I. Part of RNA polymerase I complex. Human ortholog(s) of this gene implicated in Treacher Collins syndrome 4. Orthologous to human POLR1B (RNA polymerase I subunit B). [provided by Alliance of Genome Resources, Jun 2025]
Orthologs: all
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Genomic context

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See RPA135 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (577585..581196, complement)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data show that a mutation in the universally conserved hinge region of the trigger loop, E1103G, induces a gain of function in the RNA Polymerase II (Pol II) elongation rate, whereas the corresponding mutation in RNA Polymerase I (Pol I), E1224G, results in a loss of function.
Title: A Novel Assay for RNA Polymerase I Transcription Elongation Sheds Light on the Evolutionary Divergence of Eukaryotic RNA Polymerases.

Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 5'-3' RNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to DNA-directed RNA polymerase activity	IBA Inferred from Biological aspect of Ancestor more info
contributes_to DNA-directed RNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-directed RNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotidyltransferase activity	IEA Inferred from Electronic Annotation more info
enables ribonucleoside binding	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in DNA-templated transcription initiation	IEA Inferred from Electronic Annotation more info
involved_in nucleolar large rRNA transcription by RNA polymerase I	IGI Inferred from Genetic Interaction more info	PubMed
involved_in ribosome biogenesis	IEA Inferred from Electronic Annotation more info
involved_in transcription by RNA polymerase I	IDA Inferred from Direct Assay more info	PubMed
involved_in transcription elongation by RNA polymerase I	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of DNA-directed RNA polymerase complex	IEA Inferred from Electronic Annotation more info
part_of RNA polymerase I complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RNA polymerase I complex	IDA Inferred from Direct Assay more info	PubMed
part_of intracellular protein-containing complex	IEA Inferred from Electronic Annotation more info
located_in nuclear lumen	IEA Inferred from Electronic Annotation more info
located_in nucleolus	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of transferase complex, transferring phosphorus-containing groups	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA-directed RNA polymerase I core subunit RPA135

NP_015335.1

EC 2.7.7.6

RNA polymerase I second largest subunit A135

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

577585..581196 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184107.1 → NP_015335.1 TPA: DNA-directed RNA polymerase I core subunit RPA135 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015335.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W421, P22138

UniProtKB/TrEMBL

B3LL71, C7GV35, C8ZJ35

Conserved Domains (1) summary

COG0085
Location:23 → 1203

RpoB; DNA-directed RNA polymerase, beta subunit/140 kD subunit [Transcription]