U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SAR1 Arf family GTPase SAR1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 855883, updated on 3-May-2025
Official Symbol
SAR1
Official Full Name
Arf family GTPase SAR1
Primary source
SGD:S000006139
Locus tag
YPL218W
See related
AllianceGenome:SGD:S000006139; FungiDB:YPL218W; VEuPathDB:YPL218W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables GTPase activity. Involved in several processes, including mitochondrion organization; nuclear envelope organization; and regulation of COPII vesicle coating. Located in endoplasmic reticulum exit site and mitochondria-associated endoplasmic reticulum membrane contact site. Part of COPII vesicle coat. Used to study chylomicron retention disease. Human ortholog(s) of this gene implicated in chylomicron retention disease. Orthologous to human SAR1A (secretion associated Ras related GTPase 1A) and SAR1B (secretion associated Ras related GTPase 1B). [provided by Alliance of Genome Resources, May 2025]
Orthologs
all
NEW
Try the new Gene table
Try the new Transcript table
See SAR1 in Genome Data Viewer
Location:
chromosome: XVI
Exon count:
2
Sequence:
Chromosome: XVI; NC_001148.4 (138698..139409)

Chromosome XVI - NC_001148.4Genomic Context describing neighboring genes Neighboring gene ribosomal 60S subunit protein L1A Neighboring gene Pcl8p Neighboring gene GTPase BMS1 Neighboring gene uncharacterized protein

Genomic Sequence:
NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. The GTP-binding Sar1 protein is localized to the early compartment of the yeast secretory pathway. Nishikawa S, et al. Biochim Biophys Acta, 1991 Jul 10. PMID 1907491
  2. Exit of GPI-anchored proteins from the ER differs in yeast and mammalian cells. Rivier AS, et al. Traffic, 2010 Aug. PMID 20477992
  3. Activities of mutant Sar1 proteins in guanine nucleotide binding, GTP hydrolysis, and cell-free transport from the endoplasmic reticulum to the Golgi apparatus. Saito Y, et al. J Biochem, 1998 Oct. PMID 9756629
  4. Isolation of a tobacco cDNA encoding Sar1 GTPase and analysis of its dominant mutations in vesicular traffic using a yeast complementation system. Takeuchi M, et al. Plant Cell Physiol, 1998 Jun. PMID 9697342
  5. The vesicle trafficking protein Sar1 lowers lipid membrane rigidity. Settles EI, et al. Biophys J, 2010 Sep 8. PMID 20816066, Free PMC Article

See all (124) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Structural basis for the initiation of COPII vesicle biogenesis.
    Title: Structural basis for the initiation of COPII vesicle biogenesis.
  2. It demonstrates that, besides its well-established role in ER-to-Golgi trafficking, the nuclear localization of Sar1 is essential for the viability of Saccharomyces cerevisiae.
    Title: Implication of a Novel Function of Sar1 in the Nucleus.
  3. The product of AN11127 accelerates nucleotide exchange on SAR1, but not on its closely related GTPase ArfA(ARF1), as expected for a bona fide guanine nucleotide exchange factor.
    Title: Identification of the guanine nucleotide exchange factor for SAR1 in the filamentous fungal model Aspergillus nidulans.
  4. Sar1 is a novel regulator of endoplasmic reticulum-mitochondrial contact sites in the metazoans.
    Title: Sar1, a Novel Regulator of ER-Mitochondrial Contact Sites.
  5. demonstrated that the chromosomal deletion of NEL1 leads to a significant growth defect in the temperature-sensitive sar1D32G background, suggesting a possible functional link between these proteins
    Title: Sec23 homolog Nel1 is a novel GTPase-activating protein for Sar1 but does not function as a subunit of the coat protein complex II (COPII) coat.
  6. The structure of Sec12 implicates potassium ion coordination in Sar1 activation.
    Title: The structure of Sec12 implicates potassium ion coordination in Sar1 activation.
  7. Data suggest that Sed4p appears to accelerate the dissociation of the Sec23/24p coat from the membrane, but the effect is limited to Sar1p molecules that do not capture cargo protein.
    Title: Sed4p stimulates Sar1p GTP hydrolysis and promotes limited coat disassembly.
  8. Thus, the initiation of vesicle budding by Sar1p couples the generation of membrane curvature with coat-protein assembly and cargo capture.
    Title: Sar1p N-terminal helix initiates membrane curvature and completes the fission of a COPII vesicle.
  9. a model for the maintenance of kinetically stable prebudding complexes during the Sar1p GTPase cycle that regulates cargo sorting into transport vesicles
    Title: Dissection of COPII subunit-cargo assembly and disassembly kinetics during Sar1p-GTP hydrolysis.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables GTP binding IEA
Inferred from Electronic Annotation
more info
  
enables GTPase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables GTPase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables GTPase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables membrane scission GTPase motor activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in endoplasmic reticulum to Golgi vesicle-mediated transport IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within intracellular protein transport IEA
Inferred from Electronic Annotation
more info
  
involved_in membrane organization IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in membrane organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mitochondrial fission IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in mitochondrial membrane organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in nuclear envelope organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within protein transport IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of COPII vesicle coating IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of COPII vesicle coating IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in vesicle organization IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in vesicle organization IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within vesicle-mediated transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of COPII vesicle coat IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of COPII vesicle coat IDA
Inferred from Direct Assay
more info
 PubMed 
located_in ER to Golgi transport vesicle membrane IEA
Inferred from Electronic Annotation
more info
  
located_in Golgi apparatus IEA
Inferred from Electronic Annotation
more info
  
located_in Golgi membrane IEA
Inferred from Electronic Annotation
more info
  
located_in cytoplasmic vesicle IEA
Inferred from Electronic Annotation
more info
  
located_in endoplasmic reticulum IEA
Inferred from Electronic Annotation
more info
  
is_active_in endoplasmic reticulum exit site IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in endoplasmic reticulum exit site IDA
Inferred from Direct Assay
more info
 PubMed 
located_in endoplasmic reticulum membrane IEA
Inferred from Electronic Annotation
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in mitochondria-associated endoplasmic reticulum membrane contact site IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in mitochondria-associated endoplasmic reticulum membrane contact site IMP
Inferred from Mutant Phenotype
more info
 PubMed 
located_in mitochondrion IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
Arf family GTPase SAR1
NP_015106.1
  • ARF family GTPase; component of the COPII vesicle coat; required for transport vesicle formation during ER to Golgi protein transport; lowers membrane rigidity aiding vesicle formation; localizes to ER-mitochondrial contact sites where it enhances membrane curvature, thereby reducing contact size via its N-terminal amphipathic helix; regulates mitochondrial fission and fusion dynamics

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001148.4 Reference assembly

    Range
    138698..139409
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001184032.1 → NP_015106.1  TPA: Arf family GTPase SAR1 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_015106.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6W3F2, P20606
    UniProtKB/TrEMBL
    A6ZW37, B3LKL2, C8ZIG2, G2WNW7, N1NVN1
    Conserved Domains (1) summary
    smart00178
    Location:7 → 190
    SAR; Sar1p-like members of the Ras-family of small GTPases
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P20606.1

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Molecular Biology Databases
Research Materials
Tools
Miscellaneous