VPS27 ESCRT-0 subunit protein VPS27 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: VPS27
Official Full Name: ESCRT-0 subunit protein VPS27
Primary source: SGD:S000005289
Locus tag: YNR006W
See related: AllianceGenome:SGD:S000005289; FungiDB:YNR006W; VEuPathDB:YNR006W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: DID7; GRD11; SSV17; VPL23; VPT27
Summary: Enables phosphatidylinositol-3-phosphate binding activity; polyubiquitin modification-dependent protein binding activity; and ubiquitin binding activity. Involved in several processes, including ATP export; microlipophagy; and vacuolar transport. Located in endosome and vacuolar membrane. Part of ESCRT-0 complex. Used to study Parkinson's disease. Orthologous to human HGS (hepatocyte growth factor-regulated tyrosine kinase substrate). [provided by Alliance of Genome Resources, Mar 2025]
Orthologs: all
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Genomic context

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See VPS27 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (636986..638854)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The toxic effects of yeast Ppz1 phosphatase are counteracted by subcellular relocalization mediated by its regulatory subunit Hal3.
Title: The toxic effects of yeast Ppz1 phosphatase are counteracted by subcellular relocalization mediated by its regulatory subunit Hal3.
The PI3 Kinase Complex II-PI3P-Vps27 Axis on Vacuolar Membranes is Critical for Microautophagy Induction and Nutrient Stress Adaptation.
Title: The PI3 Kinase Complex II-PI3P-Vps27 Axis on Vacuolar Membranes is Critical for Microautophagy Induction and Nutrient Stress Adaptation.
TORC1 regulates ESCRT-0 complex formation on the vacuolar membrane and microautophagy induction in yeast.
Title: TORC1 regulates ESCRT-0 complex formation on the vacuolar membrane and microautophagy induction in yeast.
Nhx1p contributes to MVB formation by the recruitment of Vps27p to the endosomal membrane, possibly through Nhx1p antiporter activity.
Title: The endosomal Na(+)/H(+) exchanger contributes to multivesicular body formation by regulating the recruitment of ESCRT-0 Vps27p to the endosomal membrane.
PSDP motifs of the Vps27 ESCRT-0 subunit bind to a novel electropositive N-terminal site on the UEV domain of the ESCRT-I subunit Vps23 centred on Trp16.
Title: Structural basis for endosomal recruitment of ESCRT-I by ESCRT-0 in yeast.

Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables K48-linked polyubiquitin modification-dependent protein binding	IDA Inferred from Direct Assay more info	PubMed
enables K63-linked polyubiquitin modification-dependent protein binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol-3-phosphate binding	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatidylinositol-3-phosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-3-phosphate binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin binding	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin binding	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in ATP export	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to nitrogen starvation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within intracellular protein transport	IEA Inferred from Electronic Annotation more info
involved_in late endosome to vacuole transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microautophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microlipophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein retention in Golgi apparatus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein targeting to vacuole	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein targeting to vacuole	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein targeting to vacuole	IPI Inferred from Physical Interaction more info	PubMed
involved_in protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within vacuolar transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within vesicle-mediated transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of ESCRT-0 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of ESCRT-0 complex	IPI Inferred from Physical Interaction more info	PubMed
located_in bounding membrane of organelle	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in endosome	IDA Inferred from Direct Assay more info	PubMed
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in endosome membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in vacuolar membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: ESCRT-0 subunit protein VPS27

NP_014403.3

Ubiquitin binding protein involved in endosomal protein sorting; K48-, K63-Ub chain binding protein that preferentially binds K63-linked chains in vitro; subunit of the ESCRT-0 complex that binds to ubiquitin via two ubiquitin-interacting motifs (UIMs) and is involved in sorting cargo into multivesicular bodies; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

636986..638854

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183183.3 → NP_014403.3 TPA: ESCRT-0 subunit protein VPS27 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014403.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W1I1, P40343

UniProtKB/TrEMBL

A6ZS80, B5VR37, C7GLP9, C8ZFN3, N1NXQ1

Conserved Domains (3) summary

cl28890
Location:169 → 226

FYVE_like_SF; FYVE domain like superfamily

cd16979
Location:7 → 149

VHS_Vps27; VHS (Vps27/Hrs/STAM) domain of Vacuolar protein sorting-associated protein 27

cd21385
Location:349 → 432

GAT_Vps27; non-canonical GAT domain found in fungal vacuolar protein sorting-associated protein 27 (Vps27) and similar proteins