LEU4 2-isopropylmalate synthase LEU4 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: LEU4
Official Full Name: 2-isopropylmalate synthase LEU4
Primary source: SGD:S000005048
Locus tag: YNL104C
See related: AllianceGenome:SGD:S000005048; FungiDB:YNL104C; VEuPathDB:YNL104C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables 2-isopropylmalate synthase activity. Involved in L-leucine biosynthetic process. Located in mitochondrion. [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See LEU4 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (424895..426754, complement)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Influence of mutation in the regulatory domain of alpha-isopropylmalate synthase from Saccharomyces cerevisiae on its activity and feedback inhibition.
Title: Influence of mutation in the regulatory domain of α-isopropylmalate synthase from Saccharomyces cerevisiae on its activity and feedback inhibition.
Mutation from Asp578 to Tyr in LEU4p is a novel change causing release from leucine feedback inhibition.
Title: Asp578 in LEU4p is one of the key residues for leucine feedback inhibition release in sake yeast.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 2-isopropylmalate synthase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 2-isopropylmalate synthase activity	IDA Inferred from Direct Assay more info	PubMed
enables 2-isopropylmalate synthase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in L-leucine biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in L-leucine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in L-leucine biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in L-leucine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: 2-isopropylmalate synthase LEU4

NP_014295.1

EC 2.3.3.13

Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

424895..426754 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182942.1 → NP_014295.1 TPA: 2-isopropylmalate synthase LEU4 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014295.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W175, P06208

UniProtKB/TrEMBL

B3LNW3, C8ZGC6, D5KUG5, N1NXG3

Conserved Domains (1) summary

TIGR00970
Location:35 → 596

leuA_yeast; 2-isopropylmalate synthase, yeast type