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IST1 Ist1p [ Saccharomyces cerevisiae S288C ]

Gene ID: 855456, updated on 12-Aug-2025
Official Symbol
IST1
Official Full Name
Ist1p
Primary source
SGD:S000005209
Locus tag
YNL265C
See related
AllianceGenome:SGD:S000005209; FungiDB:YNL265C; VEuPathDB:YNL265C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables ATPase inhibitor activity. Involved in endosome to plasma membrane protein transport and late endosome to vacuole transport via multivesicular body sorting pathway. Located in cytoplasm. Is active in endosome. Orthologous to human IST1 (IST1 factor associated with ESCRT-III). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs
all
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See IST1 in Genome Data Viewer
Location:
chromosome: XIV
Exon count:
2
Sequence:
Chromosome: XIV; NC_001146.8 (144280..145281, complement)

Chromosome XIV - NC_001146.8Genomic Context describing neighboring genes Neighboring gene lysine permease Neighboring gene 1-phosphatidylinositol 4-kinase Neighboring gene phosphatidylinositol transporter Neighboring gene protein transporter YIF1

Genomic Sequence:
NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Structural Fine-Tuning of MIT-Interacting Motif 2 (MIM2) and Allosteric Regulation of ESCRT-III by Vps4 in Yeast. Kojima R, et al. J Mol Biol, 2016 Jun 5. PMID 27075672
  2. Ist1 regulates Vps4 localization and assembly. Dimaano C, et al. Mol Biol Cell, 2008 Feb. PMID 18032582, Free PMC Article
  3. Recycling of cell surface membrane proteins from yeast endosomes is regulated by ubiquitinated Ist1. Laidlaw KME, et al. J Cell Biol, 2022 Nov 7. PMID 36125415, Free PMC Article
  4. Conformational Changes in the Endosomal Sorting Complex Required for the Transport III Subunit Ist1 Lead to Distinct Modes of ATPase Vps4 Regulation. Tan J, et al. J Biol Chem, 2015 Dec 11. PMID 26515066, Free PMC Article
  5. Structural basis of Ist1 function and Ist1-Did2 interaction in the multivesicular body pathway and cytokinesis. Xiao J, et al. Mol Biol Cell, 2009 Aug. PMID 19477918, Free PMC Article

See all (42) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Recycling of cell surface membrane proteins from yeast endosomes is regulated by ubiquitinated Ist1.
    Title: Recycling of cell surface membrane proteins from yeast endosomes is regulated by ubiquitinated Ist1.
  2. data support a model in which Ist1-Did2 interactions during ESCRT-III polymerization coordinate Vps4 activity with the timing of ESCRT-III disassembly
    Title: Conformational Changes in the Endosomal Sorting Complex Required for the Transport III Subunit Ist1 Lead to Distinct Modes of ATPase Vps4 Regulation.
  3. Cocrystallization of Ist1NTD with a Did2 fragment shows that Ist1 interacts with the Did2 C-terminal MIM1 (MIT-interacting motif 1) via a novel MIM-binding structural motif.
    Title: Structural basis of Ist1 function and Ist1-Did2 interaction in the multivesicular body pathway and cytokinesis.
  4. A model in which the Ist1-Did2 and Vta1-Vps60 complexes independently modulate late steps in the multivesicular body-sorting pathway, is proposed.
    Title: Novel Ist1-Did2 complex functions at a late step in multivesicular body sorting.
  5. Ist1 has a dual role in the regulation of Vps4 activity.
    Title: Ist1 regulates Vps4 localization and assembly.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables ATPase inhibitor activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ATPase inhibitor activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in endosome to plasma membrane protein transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in intracellular protein localization IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in late endosome to vacuole transport via multivesicular body sorting pathway IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in late endosome to vacuole transport via multivesicular body sorting pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in endosome HDA PubMed 
is_active_in endosome IDA
Inferred from Direct Assay
more info
 PubMed 
located_in endosome IDA
Inferred from Direct Assay
more info
 PubMed 
located_in endosome IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
Ist1p
NP_014134.1
  • Protein involved in recycling of membrane transporters from endosome to plasma membrane; plays a role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001146.8 Reference assembly

    Range
    144280..145281 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001183103.1 → NP_014134.1  TPA: Ist1p

    See identical proteins and their annotated locations for NP_014134.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6W0S8, P53843
    UniProtKB/TrEMBL
    A0A0L8VI73, A6ZRH8, B3LPA5, C7GP97, C8ZFX4, G2WLK0, N1NYD2
    Conserved Domains (1) summary
    pfam03398
    Location:13 → 183
    Ist1; Regulator of Vps4 activity in the MVB pathway
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P53843.1

Gene LinkOut

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