CLA4 serine/threonine protein kinase CLA4 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: CLA4
Official Full Name: serine/threonine protein kinase CLA4
Primary source: SGD:S000005242
Locus tag: YNL298W
See related: AllianceGenome:SGD:S000005242; FungiDB:YNL298W; VEuPathDB:YNL298W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: ERC10
Summary: Enables protein serine/threonine kinase activity. Involved in several processes, including negative regulation of macromolecule biosynthetic process; negative regulation of sterol import; and positive regulation of protein import into nucleus. Located in cellular bud; fungal-type vacuole; and nucleus. Human ortholog(s) of this gene implicated in breast cancer; ductal carcinoma in situ; non-syndromic X-linked intellectual disability 30; renal cell carcinoma (multiple); and schizophrenia. Orthologous to several human genes including BUB1B-PAK6 (BUB1B-PAK6 readthrough); PAK1 (p21 (RAC1) activated kinase 1); and PAK2 (p21 (RAC1) activated kinase 2). [provided by Alliance of Genome Resources, May 2025]
Orthologs: all
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Genomic context

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See CLA4 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (68915..71443)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cla4 phosphorylates histone methyltransferase Set1 to prevent its degradation by the APC/C[Cdh1] complex.
Title: Cla4 phosphorylates histone methyltransferase Set1 to prevent its degradation by the APC/C(Cdh1) complex.
Cla4p Kinase Activity Is Down-Regulated by Fus3p during Yeast Mating.
Title: Cla4p Kinase Activity Is Down-Regulated by Fus3p during Yeast Mating.
Upon arrival at the bud cortex, Vac17 is phosphorylated by Cla4. Cla4-dependent phosphorylation is required for the ubiquitylation and subsequent degradation of Vac17 and the release of the vacuole from Myo2.
Title: Spatial regulation of organelle release from myosin V transport by p21-activated kinases.
Results suggest that the Cdc42 effectors Ste20, Cla4, and Skm1 translocate into the nucleus, where they modulate expression of sterol uptake genes via Sut1, thereby controlling sterol homeostasis.
Title: The Cdc42 effectors Ste20, Cla4, and Skm1 down-regulate the expression of genes involved in sterol uptake by a mitogen-activated protein kinase-independent pathway.
Ste20p & Cla4p may function as negative modulators of sterol biosynthesis. Cla4p has negative effect on steryl ester formation. Ste20p and Cla4p may regulate cell polarity in part through the modulation of sterol homeostasis.
Title: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae.
Cla4 and Ste20 play roles in cell segregation and the degradation of Vac17.
Title: p21-activated kinases Cla4 and Ste20 regulate vacuole inheritance in Saccharomyces cerevisiae.
Hyperpolarized growth of Saccharomyces cerevisiae cak1P212S and cla4 mutants weakens cell walls and renders cells dependent on chitin synthase 3.
Title: Hyperpolarized growth of Saccharomyces cerevisiae cak1P212S and cla4 mutants weakens cell walls and renders cells dependent on chitin synthase 3.
Sec14p regulation of phosphoinositide levels affects cytokinesis at the level of the Cdc42p/Cla4p/Ste20p signaling cascade.
Title: Regulation of phosphoinositide levels by the phospholipid transfer protein Sec14p controls Cdc42p/p21-activated kinase-mediated cell cycle progression at cytokinesis.
polarisome components and Cla4p are required for the initial assembly of the septin ring
Title: Septin ring assembly requires concerted action of polarisome components, a PAK kinase Cla4p, and the actin cytoskeleton in Saccharomyces cerevisiae.
Our findings suggest that Cla4-dependent phosphorylation promotes the initial association of Lte1 with Ras at the bud cortex and that Ras is required to stabilize phosphorylated forms of Lte1 at the bud cortex.
Title: Ras and the Rho effector Cla4 collaborate to target and anchor Lte1 at the bud cortex.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 3-phosphoinositide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables Rho-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables eukaryotic translation initiation factor 2alpha kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS139 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS14 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS36 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S10 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S28 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S57 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T3 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T45 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H4S1 kinase activity	IEA Inferred from Electronic Annotation more info
enables kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	HDA more info	PubMed
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables ribosomal protein S6 kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cellular response to starvation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic cell cycle	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of gene expression	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of sterol import	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein import into nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of MAPK cascade	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of exit from mitosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to pheromone	IGI Inferred from Genetic Interaction more info	PubMed
involved_in septin ring organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in septin ring organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sterol import	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vacuole inheritance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in vacuole inheritance	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cellular bud	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in fungal-type vacuole	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: serine/threonine protein kinase CLA4

NP_014101.1

EC 2.7.11.1

Cdc42p-activated signal transducing kinase; member of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p; CLA4 has a paralog, SKM1, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

68915..71443

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183136.1 → NP_014101.1 TPA: serine/threonine protein kinase CLA4 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014101.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0P6, P48562

UniProtKB/TrEMBL

A6ZSI1, B3LPD4, N1NY59

Conserved Domains (3) summary

cd13279
Location:62 → 174

PH_Cla4_Ste20; Pleckstrin homology (PH) domain

cd06614
Location:546 → 826

STKc_PAK; Catalytic domain of the Serine/Threonine Kinase, p21-activated kinase

pfam00786
Location:183 → 241

PBD; P21-Rho-binding domain